FastQCFastQC Report
Thu 26 May 2016
SRR1545332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545332_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2200500
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83290.3785048852533515No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30630.13919563735514656No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA253.891974E-544.99999636
TCTAGCG1000.042.7499961
TAATGCG1200.041.2500041
CGTTTTT73350.041.134971
ATTACGG2050.040.6097562
ATAGCGG5100.040.147062
CACGACC6300.040.027
TATAGCG1650.039.5454521
TTACGAG403.4601544E-739.3751
TACGGGA7350.039.183674
GCGATCG750.039.09
CTATGCG1400.038.571431
CGAATAC356.2508934E-638.5714328
ACGTAGG2450.038.571432
CGTCAAT356.2508934E-638.5714322
ACTACGG659.094947E-1238.076922
CGACGTA301.1402611E-437.50000415
TACTACG301.1402611E-437.5000041
AACACGT6000.037.541
TACGCGG1150.037.1739122
Produced by FastQC (version 0.11.5)