##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545332_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2200500
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193192910702113	31.0	31.0	33.0	30.0	34.0
2	31.56206952965235	31.0	31.0	34.0	30.0	34.0
3	31.704274028629857	31.0	31.0	34.0	30.0	34.0
4	35.50197091570098	37.0	35.0	37.0	33.0	37.0
5	35.412610770279485	37.0	35.0	37.0	33.0	37.0
6	35.46552238127698	37.0	35.0	37.0	33.0	37.0
7	35.85680754374006	37.0	35.0	37.0	35.0	37.0
8	35.90971824585321	37.0	35.0	37.0	35.0	37.0
9	37.789542831174735	39.0	37.0	39.0	35.0	39.0
10	37.11316109975006	39.0	37.0	39.0	33.0	39.0
11	36.74729152465349	39.0	35.0	39.0	32.0	39.0
12	36.13598454896614	38.0	35.0	39.0	32.0	39.0
13	35.888801181549645	38.0	35.0	39.0	31.0	39.0
14	36.86477800499886	39.0	35.0	41.0	31.0	41.0
15	37.0794124062713	39.0	35.0	41.0	32.0	41.0
16	37.134982049534194	39.0	35.0	41.0	32.0	41.0
17	37.063122017723245	39.0	35.0	41.0	32.0	41.0
18	36.99253624176323	38.0	35.0	41.0	32.0	41.0
19	36.94649352419905	38.0	35.0	41.0	32.0	41.0
20	36.82440718018632	38.0	35.0	41.0	31.0	41.0
21	36.714096341740515	38.0	35.0	41.0	31.0	41.0
22	36.5940295387412	38.0	35.0	41.0	31.0	41.0
23	36.48881799591002	38.0	35.0	40.0	31.0	41.0
24	36.40886162235856	38.0	35.0	40.0	31.0	41.0
25	36.28938604862531	38.0	35.0	40.0	30.0	41.0
26	36.13644262667576	38.0	35.0	40.0	30.0	41.0
27	35.98861258804817	38.0	35.0	40.0	30.0	41.0
28	35.903898659395594	38.0	35.0	40.0	30.0	41.0
29	36.02900477164281	38.0	35.0	40.0	30.0	41.0
30	35.999218813905934	38.0	35.0	40.0	30.0	41.0
31	35.87446171324699	38.0	35.0	40.0	30.0	41.0
32	35.71666666666667	38.0	34.0	40.0	29.0	41.0
33	35.52971824585322	38.0	34.0	40.0	29.0	41.0
34	35.342905703249265	38.0	34.0	40.0	27.0	41.0
35	35.20217859577369	37.0	34.0	40.0	27.0	41.0
36	35.056200863440125	37.0	34.0	40.0	26.0	41.0
37	34.93614451261077	37.0	34.0	40.0	26.0	41.0
38	34.86895841854124	37.0	34.0	40.0	26.0	41.0
39	34.7999704612588	37.0	34.0	40.0	25.0	41.0
40	34.69367552828903	37.0	33.0	40.0	24.0	41.0
41	34.63842354010452	37.0	33.0	40.0	24.0	41.0
42	34.565600545330604	36.0	33.0	40.0	24.0	41.0
43	34.44014814814815	36.0	33.0	40.0	24.0	41.0
44	34.31948102703931	36.0	33.0	40.0	23.0	41.0
45	34.2855237446035	36.0	33.0	40.0	23.0	41.0
46	34.287775960009085	36.0	33.0	40.0	23.0	41.0
47	34.246850261304246	36.0	33.0	40.0	23.0	41.0
48	34.1705335151102	36.0	33.0	40.0	23.0	41.0
49	34.12055714610316	36.0	33.0	40.0	24.0	41.0
50	33.98933242444899	35.0	33.0	40.0	24.0	41.0
51	33.75456941604181	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	4.0
12	6.0
13	10.0
14	27.0
15	65.0
16	187.0
17	503.0
18	1094.0
19	2099.0
20	3737.0
21	5989.0
22	9153.0
23	13506.0
24	18821.0
25	26579.0
26	34467.0
27	40684.0
28	45271.0
29	51669.0
30	60447.0
31	73315.0
32	91181.0
33	116689.0
34	193378.0
35	266444.0
36	167776.0
37	208767.0
38	295178.0
39	473212.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.932106339468305	23.799772778913884	28.202772097250623	15.065348784367188
2	31.642263122017724	25.04517155192002	29.915928198136786	13.39663712792547
3	28.920608952510797	25.26943876391729	32.22281299704613	13.58713928652579
4	25.942104067257443	28.25498750284026	30.984367189275165	14.81854124062713
5	24.23694614860259	32.27511929107021	29.37414224039991	14.11379231992729
6	22.546784821631448	40.755691888207224	26.039218359463757	10.65830493069757
7	88.57577823221995	3.6238127698250397	6.253624176323563	1.5467848216314475
8	89.55010224948875	2.821404226312202	5.701204271756419	1.9272892524426266
9	84.804271756419	4.699295614633038	7.719381958645763	2.777050670302204
10	46.64180867984549	27.634764826175868	13.341195182912974	12.382231311065667
11	37.482163144739836	23.68198136787094	23.37864121790502	15.457214269484206
12	33.43217450579414	22.72138150420359	26.80322653942286	17.043217450579416
13	22.90611224721654	32.161781413315154	27.75669166098614	17.175414678482163
14	19.589093387866395	33.78454896614406	29.12047261985912	17.505885026130425
15	19.03290161326971	24.331379231992727	39.80608952510793	16.82962962962963
16	21.135287434673938	20.985730515791865	38.42208588957055	19.456896159963645
17	21.059486480345377	21.914928425357875	28.848670756646218	28.176914337650533
18	24.267302885707796	23.63340149965917	30.69911383776415	21.400181776868894
19	27.99295614633038	25.292615314701205	25.806589411497388	20.90783912747103
20	29.877527834583052	24.311020222676664	25.126607589184275	20.68484435355601
21	25.708702567598273	26.193592365371504	27.092569870483977	21.00513519654624
22	25.34401272438082	23.569688707112018	26.283571915473757	24.802726653033403
23	22.773687798227677	28.36100886162236	26.186139513746877	22.67916382640309
24	22.738104976141784	24.935696432628948	32.99531924562599	19.33087934560327
25	21.513565098841173	25.89861395137469	30.680209043399227	21.907611906384915
26	21.32006362190411	30.1702340377187	26.864667121108837	21.64503521926835
27	21.65748693478755	28.668847989093386	28.391547375596453	21.28211770052261
28	20.129152465348785	27.549147920927062	32.91979095660078	19.401908657123382
29	20.769779595546467	24.60618041354238	32.22008634401272	22.403953646898433
30	22.82326743921836	26.97196091797319	29.414996591683707	20.789775051124746
31	27.238900249943192	26.045807770961144	25.4595319245626	21.25576005453306
32	28.05712338104976	26.215587366507613	26.089843217450582	19.63744603499205
33	26.62026812088162	26.994274028629857	25.451760963417403	20.93369688707112
34	22.01854124062713	26.814633037945924	28.893069756873434	22.27375596455351
35	22.825221540558964	26.39404680754374	28.905748693478756	21.87498295841854
36	27.499522835719155	26.303067484662577	25.819768234492162	20.37764144512611
37	23.11233810497614	29.480708929788683	27.516291751874572	19.8906612133606
38	23.01299704612588	29.242990229493298	25.584185412406274	22.15982731197455
39	22.71438309475119	29.070756646216765	26.30688479890934	21.9079754601227
40	24.29347875482845	26.112883435582823	26.221313337877756	23.372324471710975
41	20.303567371052033	25.78509429675074	26.9634174051352	26.94792092706203
42	23.508066348557147	26.74587593728698	25.040172688025446	24.705885026130424
43	23.288525335151103	26.684753465121563	26.428902522154058	23.59781867757328
44	22.626539422858443	28.93197000681663	26.688707112019994	21.75278345830493
45	20.957555101113385	30.209406952965235	24.869120654396728	23.963917291524652
46	23.65071574642127	28.48234492160873	26.389047943649167	21.477891388320835
47	23.058895705521472	26.370824812542605	27.562872074528517	23.007407407407406
48	24.20295387411952	24.222176778005	28.376050897523292	23.198818450352192
49	22.51624630765735	24.70802090433992	28.967598273119744	23.80813451488298
50	21.220995228357193	28.589184276300838	27.50824812542604	22.68157236991593
51	20.93583276528062	29.593047034764826	25.585866848443533	23.88525335151102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	749.0
1	1725.0
2	2701.0
3	9188.0
4	15675.0
5	11649.5
6	7624.0
7	7717.0
8	7810.0
9	8071.0
10	8332.0
11	8590.0
12	8848.0
13	8629.5
14	8411.0
15	8221.0
16	8031.0
17	7992.5
18	7954.0
19	8071.0
20	8188.0
21	8051.0
22	7914.0
23	9219.0
24	10524.0
25	11526.0
26	14409.0
27	16290.0
28	18873.0
29	21456.0
30	26339.5
31	31223.0
32	37124.5
33	43026.0
34	47582.0
35	52138.0
36	59429.0
37	66720.0
38	74599.5
39	82479.0
40	100430.0
41	118381.0
42	143178.5
43	167976.0
44	181279.5
45	194583.0
46	191443.0
47	188303.0
48	181107.0
49	173911.0
50	162904.0
51	151897.0
52	145421.0
53	138945.0
54	128262.0
55	117579.0
56	110838.5
57	104098.0
58	98360.5
59	92623.0
60	88677.0
61	84731.0
62	76691.0
63	68651.0
64	60281.5
65	51912.0
66	45061.5
67	38211.0
68	32266.0
69	26321.0
70	23037.0
71	19753.0
72	16600.5
73	13448.0
74	11009.0
75	7275.5
76	5981.0
77	4444.0
78	2907.0
79	2296.0
80	1685.0
81	1180.0
82	675.0
83	543.5
84	412.0
85	291.5
86	171.0
87	123.5
88	76.0
89	68.5
90	61.0
91	36.5
92	12.0
93	7.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2200500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.841755506057986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97576498230579	16.45993584665113
2	6.774374358264108	2.82379708162898
3	2.278262977196042	1.42448999847671
4	1.2447625593094915	1.0377214769689371
5	0.7507234849829796	0.7823197663335527
6	0.5709068390827942	0.7139220454139992
7	0.4505019227976822	0.6572475650970813
8	0.394379748548786	0.6575653036635539
9	0.34093419931322433	0.6395100503165878
>10	5.433512249351075	29.16009364126194
>50	2.4257186466567306	35.18372956449834
>100	0.35730138021677776	9.577365603144345
>500	0.001977682136232828	0.2604084865567006
>1k	6.592273787442759E-4	0.2404404773870345
>5k	2.1974245958142536E-4	0.38145309260125915
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8329	0.3785048852533515	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3063	0.13919563735514656	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0359918200408998	0.0
2	0.0	0.0	0.0	0.11515564644399	0.0
3	0.0	0.0	0.0	0.1907293796864349	0.0
4	0.0	0.0	0.0	0.3289706884798909	0.0
5	0.0	0.0	0.0	0.5577823221995001	0.0
6	0.0	0.0	0.0	1.0518064076346285	0.0
7	0.0	0.0	0.0	1.3110202226766643	0.0
8	0.0	0.0	0.0	1.9428311747330151	0.0
9	0.0	0.0	0.0	2.2910702113156103	0.0
10	0.0	0.0	0.0	2.6885707793683253	0.0
11	0.0	0.0	0.0	3.045171551920018	0.0
12	0.0	0.0	0.0	3.3323790047716426	0.0
13	0.0	0.0	0.0	3.4877982276755284	0.0
14	0.0	0.0	0.0	3.5670074982958417	0.0
15	0.0	0.0	0.0	3.6439445580549874	0.0
16	0.0	0.0	0.0	3.7810043172006362	0.0
17	0.0	0.0	0.0	3.935650988411725	0.0
18	0.0	0.0	0.0	4.130015905476029	0.0
19	4.544421722335833E-5	0.0	0.0	4.251579186548511	0.0
20	4.544421722335833E-5	0.0	0.0	4.376550783912747	0.0
21	4.544421722335833E-5	0.0	0.0	4.5378777550556695	0.0
22	4.544421722335833E-5	0.0	0.0	4.7113837764144515	0.0
23	4.544421722335833E-5	0.0	0.0	4.896250852079073	0.0
24	4.544421722335833E-5	0.0	0.0	5.046262213133379	0.0
25	4.544421722335833E-5	0.0	0.0	5.1750965689615995	0.0
26	4.544421722335833E-5	0.0	0.0	5.298477618723018	0.0
27	4.544421722335833E-5	0.0	0.0	5.422994773915019	0.0
28	4.544421722335833E-5	0.0	0.0	5.555419222903885	0.0
29	9.088843444671665E-5	0.0	0.0	5.695978186775733	0.0
30	9.088843444671665E-5	0.0	0.0	5.880754374005908	0.0
31	9.088843444671665E-5	0.0	0.0	6.0394910247670985	0.0
32	9.088843444671665E-5	0.0	0.0	6.205453306066803	0.0
33	9.088843444671665E-5	0.0	0.0	6.3653715064758005	0.0
34	9.088843444671665E-5	0.0	0.0	6.5336059986366735	0.0
35	9.088843444671665E-5	0.0	0.0	6.715473755964553	0.0
36	9.088843444671665E-5	0.0	0.0	6.887616450806635	0.0
37	9.088843444671665E-5	0.0	0.0	7.0647125653260625	0.0
38	9.088843444671665E-5	0.0	0.0	7.253896841626903	0.0
39	9.088843444671665E-5	0.0	0.0	7.456623494660304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	25	3.891974E-5	44.999996	36
TCTAGCG	100	0.0	42.749996	1
TAATGCG	120	0.0	41.250004	1
CGTTTTT	7335	0.0	41.13497	1
ATTACGG	205	0.0	40.609756	2
ATAGCGG	510	0.0	40.14706	2
CACGACC	630	0.0	40.0	27
TATAGCG	165	0.0	39.545452	1
TTACGAG	40	3.4601544E-7	39.375	1
TACGGGA	735	0.0	39.18367	4
GCGATCG	75	0.0	39.0	9
CTATGCG	140	0.0	38.57143	1
CGAATAC	35	6.2508934E-6	38.57143	28
ACGTAGG	245	0.0	38.57143	2
CGTCAAT	35	6.2508934E-6	38.57143	22
ACTACGG	65	9.094947E-12	38.07692	2
CGACGTA	30	1.1402611E-4	37.500004	15
TACTACG	30	1.1402611E-4	37.500004	1
AACACGT	600	0.0	37.5	41
TACGCGG	115	0.0	37.173912	2
>>END_MODULE