FastQCFastQC Report
Thu 26 May 2016
SRR1545331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545331_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3763537
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110110.29257052607693246No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79610.2115297391788629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG950.045.0000041
ACGTTAC253.8925038E-545.028
CCGTCGA207.0353353E-445.035
ATACCCG207.0353353E-445.029
CGGACTA207.0353353E-445.014
CGCATAT302.1668238E-644.99999629
CTATACG1050.042.8571431
CGTTGAT8300.042.0180725
CATACGT556.184564E-1140.90909239
TACGAAC501.0822987E-940.534
TAATCGT8700.040.34482621
TTATACG1400.040.1785741
TTAATCG8800.040.14204420
GTCGATA850.039.7058839
CACGACC13100.039.6755727
AATCGTT8900.039.438222
GCGATAT5600.039.3750049
CGTAATT800.039.37515
CGTTTTT91050.038.822081
TCGTTGA9050.038.5359124
Produced by FastQC (version 0.11.5)