##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545331_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3763537
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262211318767424	31.0	31.0	33.0	30.0	34.0
2	31.63625015510675	31.0	31.0	34.0	30.0	34.0
3	31.827800018971516	31.0	31.0	34.0	30.0	34.0
4	35.621745979911985	37.0	35.0	37.0	33.0	37.0
5	35.54187324317524	37.0	35.0	37.0	33.0	37.0
6	35.60035546349086	37.0	35.0	37.0	33.0	37.0
7	35.861194934445976	37.0	35.0	37.0	35.0	37.0
8	35.89577118545666	37.0	35.0	37.0	35.0	37.0
9	37.82306829984666	39.0	37.0	39.0	35.0	39.0
10	37.2407538440568	39.0	37.0	39.0	34.0	39.0
11	36.85982255521867	39.0	35.0	39.0	33.0	39.0
12	36.007633245003305	37.0	35.0	39.0	32.0	39.0
13	35.624077563207166	37.0	35.0	39.0	31.0	39.0
14	36.56691139212926	38.0	35.0	41.0	31.0	41.0
15	36.84243306230283	38.0	35.0	41.0	32.0	41.0
16	36.96876023804203	38.0	35.0	41.0	32.0	41.0
17	36.9175230640751	38.0	35.0	41.0	32.0	41.0
18	36.879343819391174	38.0	35.0	40.0	32.0	41.0
19	36.83119390084381	38.0	35.0	40.0	32.0	41.0
20	36.681374728081586	38.0	35.0	40.0	32.0	41.0
21	36.55426185527072	38.0	35.0	40.0	31.0	41.0
22	36.43752034323032	38.0	35.0	40.0	31.0	41.0
23	36.365466581038	38.0	35.0	40.0	31.0	41.0
24	36.24099510646501	37.0	35.0	40.0	31.0	41.0
25	36.10812249221942	37.0	35.0	40.0	31.0	41.0
26	35.950501084485154	37.0	35.0	40.0	30.0	41.0
27	35.803828951329564	37.0	34.0	40.0	30.0	41.0
28	35.72964766920054	37.0	34.0	40.0	30.0	41.0
29	35.86044590500904	37.0	35.0	40.0	30.0	41.0
30	35.86523342270848	37.0	35.0	40.0	30.0	41.0
31	35.7375835550441	37.0	34.0	40.0	30.0	41.0
32	35.60273434272069	37.0	34.0	40.0	30.0	41.0
33	35.414390505527116	37.0	34.0	40.0	29.0	41.0
34	35.28075929637466	36.0	34.0	40.0	29.0	41.0
35	35.13202819581686	36.0	34.0	40.0	28.0	41.0
36	34.95196008435682	36.0	34.0	40.0	27.0	41.0
37	34.82876878850932	36.0	34.0	40.0	26.0	41.0
38	34.77629634038406	36.0	34.0	40.0	27.0	41.0
39	34.71226535038715	36.0	34.0	40.0	26.0	41.0
40	34.53886437146758	36.0	33.0	40.0	25.0	41.0
41	34.485675310220145	35.0	33.0	40.0	25.0	41.0
42	34.43434673287389	35.0	33.0	40.0	25.0	41.0
43	34.300658928024355	35.0	33.0	40.0	25.0	41.0
44	34.17843002473471	35.0	33.0	40.0	24.0	41.0
45	34.14243675563705	35.0	33.0	40.0	24.0	41.0
46	34.18222113931655	35.0	33.0	40.0	24.0	41.0
47	34.15353243504714	35.0	33.0	40.0	25.0	41.0
48	34.077991793358215	35.0	33.0	39.0	24.0	41.0
49	34.05302644825865	35.0	33.0	39.0	24.0	41.0
50	33.903658446828075	35.0	33.0	39.0	24.0	41.0
51	33.66597432149597	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	5.0
12	13.0
13	24.0
14	48.0
15	115.0
16	306.0
17	760.0
18	1651.0
19	3473.0
20	5980.0
21	9525.0
22	14296.0
23	21080.0
24	29832.0
25	41046.0
26	53912.0
27	64894.0
28	74364.0
29	86273.0
30	102759.0
31	126905.0
32	159824.0
33	211557.0
34	379116.0
35	560969.0
36	278713.0
37	343564.0
38	471874.0
39	720214.0
40	439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.257906857299396	23.84095068017134	28.731058044600065	13.170084417929198
2	30.80344367545742	25.92526657769008	29.541811333328194	13.729478413524298
3	29.35666634870336	25.863383301399722	30.489563408038766	14.290386941858152
4	25.995200791170646	28.734857661821845	29.787165637005824	15.482775910001681
5	23.973060448190093	32.55108160222684	28.838908718049005	14.63694923153406
6	22.465887807134617	41.105454788939234	25.620500077453734	10.808157326472411
7	88.37362300410491	4.290432112132816	5.551825317513817	1.784119566248452
8	90.17185163849857	2.8931295215112804	5.019108354720573	1.9159104852695747
9	86.02774464552893	4.293354894611107	6.976469209682275	2.702431250177692
10	54.13883269913382	21.475516249740604	12.03747963684162	12.348171414283957
11	45.96830056407045	21.368382986536336	17.96583904980873	14.697477399584486
12	39.841245084079155	21.750789217696013	22.761487398689052	15.646478299535783
13	22.936163507891646	36.819008289276816	23.83130549799298	16.41352270483856
14	18.772553584566857	37.83082244176157	27.313508542628917	16.08311543104266
15	17.789249846620347	22.134072283599178	43.927826403726066	16.148851466054406
16	19.109470692064406	17.648345160416916	42.509586062260055	20.732598085258626
17	19.54900403529977	18.83534026635051	27.69017549183122	33.92548020651849
18	23.896270981260447	22.32200719695329	31.28870527910314	22.49301654268312
19	30.76116961252141	23.813742232373432	22.91610259179065	22.50898556331451
20	32.7093635588012	22.74631550055174	23.082355773305803	21.461965167341255
21	25.57790716552009	26.696270024713453	25.424142236412184	22.30168057335427
22	25.754815217706113	23.730868063738978	23.551276365822897	26.963040352732015
23	23.643025164891434	29.090294581931836	23.174742270369602	24.091937982807128
24	22.86179197919404	23.548167588095986	34.359938536541556	19.23010189616842
25	20.811911773419524	24.501712086263534	31.519737948637143	23.166638191679795
26	19.932898228448398	31.942106587500003	25.03456190280579	23.090433281245808
27	21.873997784530882	29.562935079421298	26.952890326307404	21.610176809740413
28	18.633641704598627	27.477078078414003	34.18489043684173	19.704389780145647
29	19.68005097332642	24.007310144685704	33.381683241057544	22.93095564093033
30	21.98599349494903	27.664083015525026	28.524603318633506	21.825320170892436
31	28.41481298044898	25.890830885945853	23.47671884187667	22.217637291728497
32	30.53959612991715	25.683127334738572	23.97159905695095	19.80567747839333
33	27.603023432478548	26.210742713569708	24.52065703087282	21.66557682307893
34	21.50819827199786	25.90403654859777	27.87651616019718	24.711249019207198
35	22.764038190670107	25.449171882726272	28.754892007173037	23.031897919430577
36	30.365903138457256	25.243434567004392	23.946064566390604	20.444597728147752
37	23.335654731174422	30.074926857368478	26.14014423134408	20.449274180113015
38	23.43835067916165	30.324373056515718	22.961963705950016	23.275312558372615
39	23.76099929401518	29.68505424551426	24.04825567013158	22.50569079033898
40	25.361116417880307	25.2395286667834	23.896536688758474	25.502818226577816
41	19.673062866128326	23.819587797330012	25.54995473672771	30.957394599813952
42	23.84145552441759	26.471534622882675	22.9005852739059	26.786424578793834
43	23.195945728712115	26.44677068406661	24.978444479222603	25.378839107998672
44	22.966374450417256	28.861892416628294	25.93857852334121	22.233154609613244
45	20.151814636072395	31.700605042543756	22.9467386663131	25.20084165507075
46	22.90728110285617	29.625695190455147	25.317380963705155	22.149642742983527
47	23.802476234457107	25.419306359948102	26.80752175413713	23.970695651457657
48	24.65723068485842	22.321316357458425	28.764563760101204	24.256889197581955
49	22.07131217256533	23.12492211448964	29.60119695913711	25.20256875380792
50	20.88458277412976	30.207833747881313	25.804289953838637	23.10329352415029
51	20.22605331102099	31.159119732315638	23.474035196146602	25.140791760516766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1494.0
1	3280.0
2	5066.0
3	12967.0
4	20868.0
5	15579.0
6	10290.0
7	10649.0
8	11008.0
9	11615.5
10	12223.0
11	12096.5
12	11970.0
13	12352.5
14	12735.0
15	12324.0
16	11913.0
17	11218.5
18	10524.0
19	10354.5
20	10185.0
21	10034.5
22	9884.0
23	10177.0
24	10470.0
25	12816.5
26	16353.0
27	17543.0
28	23064.5
29	28586.0
30	33015.5
31	37445.0
32	45317.5
33	53190.0
34	60904.5
35	68619.0
36	72087.0
37	75555.0
38	93600.0
39	111645.0
40	167503.5
41	223362.0
42	266089.0
43	308816.0
44	324899.5
45	340983.0
46	331094.0
47	321205.0
48	302291.0
49	283377.0
50	274678.5
51	265980.0
52	254577.0
53	243174.0
54	222735.5
55	202297.0
56	198465.5
57	194634.0
58	189731.0
59	184828.0
60	176872.0
61	168916.0
62	150856.0
63	132796.0
64	118338.5
65	103881.0
66	90756.5
67	77632.0
68	67803.5
69	57975.0
70	48298.5
71	38622.0
72	33180.5
73	27739.0
74	23575.5
75	15016.5
76	10621.0
77	7925.5
78	5230.0
79	3989.5
80	2749.0
81	2301.5
82	1854.0
83	1285.0
84	716.0
85	468.0
86	220.0
87	151.5
88	83.0
89	53.5
90	24.0
91	24.0
92	24.0
93	15.5
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3763537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.69215709925148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91723966955458	12.54073879833183
2	8.332637446575571	2.615141117255391
3	2.715086800729773	1.278167058454671
4	1.2678902171689381	0.7958372988967619
5	0.722559413653364	0.5669257916295811
6	0.49894998744771346	0.4697760952619444
7	0.34513523337539354	0.37911414118294484
8	0.2852132501286772	0.35804889022458486
9	0.21600478790651093	0.30506229594175194
>10	2.3185633989122327	8.577317577989964
>50	1.2279506472509925	14.298626538362674
>100	2.140433371442998	55.86637499137903
>500	0.009937152770766898	0.9928512065518579
>1k	0.002055962642227634	0.4459482510513598
>5k	1.7133022018563616E-4	0.21403472759509706
>10k+	1.7133022018563616E-4	0.2960352198906687
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11011	0.29257052607693246	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7961	0.2115297391788629	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.051361259368514246	0.0
2	0.0	0.0	0.0	0.13144549927368857	0.0
3	0.0	0.0	0.0	0.1933287755640505	0.0
4	0.0	0.0	0.0	0.34098243221735297	0.0
5	0.0	0.0	0.0	0.5517416196519391	0.0
6	0.0	0.0	0.0	1.00397578129297	0.0
7	0.0	0.0	0.0	1.2265589523897333	0.0
8	0.0	0.0	0.0	1.7663703053802846	0.0
9	0.0	0.0	0.0	2.0055601951037016	0.0
10	0.0	0.0	0.0	2.3021694751506363	0.0
11	0.0	0.0	0.0	2.653886490288258	0.0
12	0.0	0.0	0.0	2.9348987402010396	0.0
13	0.0	0.0	0.0	3.083987217343685	0.0
14	0.0	0.0	0.0	3.148793276112338	0.0
15	0.0	0.0	0.0	3.2163361221106634	0.0
16	0.0	0.0	0.0	3.3572673790638965	0.0
17	0.0	0.0	0.0	3.5018122579902893	0.0
18	0.0	0.0	0.0	3.6950878920547345	0.0
19	0.0	0.0	0.0	3.8028057117546608	0.0
20	0.0	0.0	0.0	3.904199693001557	0.0
21	0.0	0.0	0.0	4.046698624193146	0.0
22	0.0	0.0	0.0	4.1938208658503955	0.0
23	0.0	0.0	0.0	4.3691081022984495	0.0
24	0.0	0.0	0.0	4.501988422061481	0.0
25	0.0	0.0	0.0	4.612230462992658	0.0
26	0.0	0.0	0.0	4.723296197167717	0.0
27	0.0	0.0	0.0	4.826948692147838	0.0
28	2.6570749802645755E-5	0.0	0.0	4.937669006575463	0.0
29	2.6570749802645755E-5	0.0	0.0	5.059814743418226	0.0
30	2.6570749802645755E-5	0.0	0.0	5.208664083812647	0.0
31	2.6570749802645755E-5	0.0	0.0	5.352438410994764	0.0
32	2.6570749802645755E-5	0.0	0.0	5.48577043350444	0.0
33	2.6570749802645755E-5	0.0	0.0	5.623247492983329	0.0
34	2.6570749802645755E-5	0.0	0.0	5.75562296850011	0.0
35	2.6570749802645755E-5	0.0	0.0	5.92554291348803	0.0
36	2.6570749802645755E-5	0.0	0.0	6.074126546384425	0.0
37	2.6570749802645755E-5	0.0	0.0	6.222550754782004	0.0
38	2.6570749802645755E-5	0.0	0.0	6.378547626873337	0.0
39	2.6570749802645755E-5	0.0	0.0	6.555960523305603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	95	0.0	45.000004	1
ACGTTAC	25	3.8925038E-5	45.0	28
CCGTCGA	20	7.0353353E-4	45.0	35
ATACCCG	20	7.0353353E-4	45.0	29
CGGACTA	20	7.0353353E-4	45.0	14
CGCATAT	30	2.1668238E-6	44.999996	29
CTATACG	105	0.0	42.857143	1
CGTTGAT	830	0.0	42.01807	25
CATACGT	55	6.184564E-11	40.909092	39
TACGAAC	50	1.0822987E-9	40.5	34
TAATCGT	870	0.0	40.344826	21
TTATACG	140	0.0	40.178574	1
TTAATCG	880	0.0	40.142044	20
GTCGATA	85	0.0	39.705883	9
CACGACC	1310	0.0	39.67557	27
AATCGTT	890	0.0	39.4382	22
GCGATAT	560	0.0	39.375004	9
CGTAATT	80	0.0	39.375	15
CGTTTTT	9105	0.0	38.82208	1
TCGTTGA	905	0.0	38.53591	24
>>END_MODULE