FastQCFastQC Report
Thu 26 May 2016
SRR1545330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545330_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3477856
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT41790.12016023665154625No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36380.10460467598428456No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC35970.10342578876181188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG1650.045.0000041
TGTAACG1250.045.0000041
TACCCGT453.8562575E-1045.012
CTCCGCG351.212884E-745.038
CTATCGT406.8212103E-945.010
GCGCGTT502.1827873E-1145.023
ACGTTAG2650.045.01
ATAGCGC207.03526E-445.032
CCGTGCA253.892442E-545.012
TGTATCG850.045.01
TCGATTG1100.045.01
CTGCGAA453.8562575E-1045.032
CGTAGAT406.8212103E-945.036
GTACTCG207.03526E-445.01
TGTTACG1950.045.01
AACGACA406.8212103E-945.022
CCGACAA207.03526E-445.012
CTATTCG551.8189894E-1245.025
CGTGTAT351.212884E-745.018
ACCGTTG351.212884E-745.010
Produced by FastQC (version 0.11.5)