##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545330_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3477856
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236908313627705	31.0	31.0	33.0	30.0	34.0
2	31.533212128391746	31.0	31.0	34.0	30.0	34.0
3	31.614091555257033	31.0	31.0	34.0	30.0	34.0
4	30.235119280384236	35.0	33.0	37.0	0.0	37.0
5	32.77737491143969	35.0	33.0	37.0	19.0	37.0
6	34.383201029599846	35.0	33.0	37.0	30.0	37.0
7	35.47341264273162	36.0	35.0	37.0	33.0	37.0
8	35.670911619112466	37.0	35.0	37.0	35.0	37.0
9	37.716760843462176	39.0	37.0	39.0	35.0	39.0
10	37.15216529954087	39.0	37.0	39.0	33.0	39.0
11	36.60382546028357	39.0	35.0	39.0	32.0	39.0
12	35.03239064527111	35.0	35.0	39.0	30.0	39.0
13	34.40053210943754	35.0	33.0	39.0	27.0	39.0
14	35.06347646366037	35.0	33.0	40.0	27.0	41.0
15	35.5657428024622	35.0	33.0	40.0	30.0	41.0
16	35.85593998141383	35.0	34.0	40.0	31.0	41.0
17	35.81092690439167	35.0	34.0	40.0	31.0	41.0
18	35.82773064784741	36.0	35.0	40.0	31.0	41.0
19	35.781767272710546	36.0	34.0	40.0	31.0	41.0
20	35.61780821287598	35.0	34.0	40.0	31.0	41.0
21	35.412453822124895	35.0	34.0	40.0	30.0	41.0
22	35.25917921846103	35.0	34.0	40.0	30.0	41.0
23	35.29161558155369	35.0	34.0	40.0	30.0	41.0
24	35.22200142846627	35.0	34.0	40.0	30.0	41.0
25	35.106429650911366	35.0	34.0	39.0	30.0	41.0
26	34.9477462551641	35.0	34.0	39.0	29.0	41.0
27	34.8769477517183	35.0	34.0	39.0	29.0	41.0
28	34.96256170468242	35.0	34.0	39.0	29.0	41.0
29	35.08855254501624	35.0	34.0	39.0	29.0	41.0
30	35.058259743934194	36.0	34.0	39.0	29.0	41.0
31	34.8705156280191	35.0	34.0	39.0	29.0	41.0
32	34.708833258191255	35.0	34.0	39.0	29.0	41.0
33	34.55642700560345	35.0	33.0	39.0	28.0	41.0
34	34.517596185696014	35.0	34.0	39.0	28.0	41.0
35	34.39383718014777	35.0	33.0	39.0	27.0	41.0
36	34.1399505902487	35.0	33.0	39.0	25.0	41.0
37	34.03921065161985	35.0	33.0	39.0	25.0	41.0
38	34.062482747991865	35.0	33.0	39.0	27.0	41.0
39	34.03710446896019	35.0	33.0	39.0	26.0	41.0
40	33.92647395406826	35.0	33.0	39.0	25.0	41.0
41	33.96636117194041	35.0	33.0	39.0	25.0	41.0
42	33.894451639170796	35.0	33.0	38.0	25.0	41.0
43	33.75519572978295	35.0	33.0	38.0	25.0	41.0
44	33.69488846001675	35.0	33.0	38.0	25.0	41.0
45	33.62964740345776	35.0	33.0	38.0	24.0	40.0
46	33.61930539964852	35.0	33.0	38.0	25.0	40.0
47	33.547846719358134	35.0	33.0	38.0	24.0	40.0
48	33.493608993586854	35.0	33.0	38.0	24.0	40.0
49	33.495860955715244	35.0	33.0	38.0	24.0	40.0
50	33.264734365080095	35.0	32.0	38.0	24.0	40.0
51	33.167113014454884	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	9.0
14	14.0
15	96.0
16	306.0
17	892.0
18	1972.0
19	3886.0
20	6832.0
21	10693.0
22	16488.0
23	23610.0
24	33246.0
25	44076.0
26	56579.0
27	68477.0
28	81900.0
29	98819.0
30	120431.0
31	151818.0
32	194831.0
33	294478.0
34	457709.0
35	648186.0
36	212632.0
37	247588.0
38	317017.0
39	385005.0
40	262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.478531601078362	25.41335811488457	30.9536967603029	12.154413523734164
2	26.49618040539919	27.195490555100616	32.31306874120148	13.995260298298723
3	28.31388073571764	25.95446734999954	31.734120101579823	13.997531812702999
4	21.25924707635969	39.196188686363094	26.6139541142589	12.930610123018319
5	23.033357332793535	32.06843526586495	30.365892089839257	14.53231531150226
6	21.341251621688766	40.82745231544952	26.35022841658769	11.481067646274026
7	88.9274599063331	4.413609994203325	4.723111020122742	1.9358190793408354
8	91.15210635517974	2.7251847115004186	3.614554484141954	2.5081544491778844
9	88.84378766688442	3.4985922361362864	5.272759999263914	2.384860097715374
10	66.63496130949642	16.758773221202947	8.094987256516658	8.511278212783967
11	59.769438412631224	15.993157853574155	14.186815095277092	10.050588638517523
12	52.03611075329169	20.928439820395095	16.329083205285094	10.706366221028128
13	18.9453214854209	53.50520550592089	17.192891252541795	10.356581756116412
14	11.609393833442212	52.64979343595595	24.569504890369238	11.171307840232602
15	9.624464037613977	21.807947195053504	57.19840039380584	11.36918837352668
16	12.007483921128419	15.48465491383197	53.799639778070166	18.70822138696944
17	13.052898107339693	17.25821885667492	25.45490670114001	44.23397633484537
18	21.046328542642364	22.713993908890995	34.9069081641103	21.33276938435634
19	33.88708445663075	23.49375592318946	21.707655521102655	20.91150409907713
20	36.1586276142543	23.344411039444992	21.523174047459122	18.973787298841586
21	21.55069100043245	31.15870812362559	25.040455959073636	22.250144916868322
22	22.290830902717076	25.732606525399554	21.8587256056605	30.117836966222868
23	18.091404589494218	34.698417645813976	21.392806372661777	25.81737139203003
24	19.5280080601382	23.774331082137962	41.90716924450006	14.79049161322378
25	14.649168913261503	25.762970059714952	38.296323942106866	21.291537084916683
26	14.207661271772034	41.38112676315523	24.109997653726893	20.30121431134584
27	16.710093804918895	38.72621523145294	27.405878794291656	17.157812169336513
28	11.89986014372073	29.300580587580395	43.44164910795617	15.35791016074271
29	11.951903701590865	24.671694285214798	41.87019244040006	21.506209572794273
30	17.048606957849895	33.33640035700156	31.379102527534204	18.235890157614346
31	32.90199479219381	27.042091449444715	21.2672117534481	18.78870200491337
32	35.336540673334376	26.23622139617051	22.71968707157513	15.707550858919978
33	30.09466176862987	27.75261540443308	21.750239227846123	20.402483599090935
34	16.922926078595548	27.827949173283773	27.575236007471272	27.67388874064941
35	18.207884397743896	24.78538501881619	32.24276680805646	24.763963775383456
36	35.42826384991213	21.39556669396318	25.510745700799575	17.66542375532512
37	19.20709195550362	33.73210391689593	29.475602210097257	17.5852019175032
38	18.432936843848623	36.34483428871121	21.8308636125245	23.391365254915673
39	19.40687020969241	34.462755214706995	26.17877220908514	19.951602366515463
40	25.484263868314272	25.46672432671163	21.917497446702797	27.131514358271303
41	15.4431925876172	21.62760620336207	26.494196424463805	36.435004784556924
42	22.643778235786645	23.988773543240434	21.675854319442784	31.69159390153014
43	21.800873871718668	25.863290487012687	24.391234139653857	27.944601501614784
44	18.89431304803879	31.90071699345804	28.017951289530103	21.187018668973067
45	15.201721980438524	42.226935215259054	19.088886946440567	23.482455857861854
46	22.410761112593505	34.52333851660333	23.71173504595935	19.354165324843812
47	20.287326444798172	25.984830884314935	27.233358712954185	26.4944839579327
48	23.996048140003495	20.001058123165535	33.42746220660085	22.57543153023012
49	20.736166189744488	19.948813291867175	34.86429570401995	24.450724814368392
50	18.671934663194794	35.85775259240175	24.540291489929427	20.93002125447402
51	16.0037965919233	37.3990182457238	21.95740709218553	24.639778070167367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1099.0
1	1311.0
2	1523.0
3	3955.0
4	6387.0
5	5117.5
6	3848.0
7	4671.5
8	5495.0
9	6482.0
10	7469.0
11	8075.0
12	8681.0
13	8639.0
14	8597.0
15	8359.5
16	8122.0
17	8119.5
18	8117.0
19	7876.5
20	7636.0
21	8256.5
22	8877.0
23	9542.0
24	10207.0
25	11660.0
26	15316.5
27	17520.0
28	20726.0
29	23932.0
30	29052.5
31	34173.0
32	40498.5
33	46824.0
34	55273.5
35	63723.0
36	76398.5
37	89074.0
38	112935.5
39	136797.0
40	224456.0
41	312115.0
42	405852.0
43	499589.0
44	476125.0
45	452661.0
46	419097.0
47	385533.0
48	346939.0
49	308345.0
50	281855.0
51	255365.0
52	222242.0
53	189119.0
54	168565.5
55	148012.0
56	124298.0
57	100584.0
58	91345.5
59	82107.0
60	79126.0
61	76145.0
62	65878.5
63	55612.0
64	44912.5
65	34213.0
66	29978.5
67	25744.0
68	21186.0
69	16628.0
70	13865.5
71	11103.0
72	8908.5
73	6714.0
74	5582.0
75	2966.5
76	1483.0
77	1021.0
78	559.0
79	433.5
80	308.0
81	264.5
82	221.0
83	117.0
84	13.0
85	11.0
86	9.0
87	6.5
88	4.0
89	5.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3477856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.725232347635911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.26469610553137	11.23015370075793
2	10.387931607635084	3.0592941306755526
3	4.00759489267062	1.7703829784932166
4	2.1662596154527107	1.2759470465136633
5	1.301705572337162	0.9583958500438545
6	0.8037300449526339	0.7101066994022033
7	0.5675192417898536	0.5849796887976831
8	0.4071941598447807	0.47968228914518335
9	0.29488747022081135	0.39080578638672114
>10	1.723552763402147	5.257628917426215
>50	0.4564472700120929	4.895706996094738
>100	1.4867493363410718	55.70183951537776
>500	0.1192364483164737	10.878316249570478
>1k	0.012495471493200619	2.8067601513148492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	4179	0.12016023665154625	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3638	0.10460467598428456	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	3597	0.10342578876181188	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1409201531058215	0.0
2	0.0	0.0	0.0	0.45090998592236137	0.0
3	0.0	0.0	0.0	0.6307621707166714	0.0
4	0.0	0.0	0.0	1.0408999107496113	0.0
5	0.0	0.0	0.0	1.6321549828400026	0.0
6	0.0	0.0	0.0	2.245808912157375	0.0
7	0.0	0.0	0.0	2.52069090842174	0.0
8	0.0	0.0	0.0	3.1689638673941647	0.0
9	2.8753346889577946E-5	0.0	0.0	3.3476946716597813	0.0
10	2.8753346889577946E-5	0.0	0.0	3.653342749095995	0.0
11	2.8753346889577946E-5	0.0	0.0	4.120613389398526	0.0
12	2.8753346889577946E-5	0.0	0.0	4.50320542311125	0.0
13	2.8753346889577946E-5	0.0	0.0	4.650566325920337	0.0
14	2.8753346889577946E-5	0.0	0.0	4.71031578075688	0.0
15	2.8753346889577946E-5	0.0	0.0	4.810693714748397	0.0
16	2.8753346889577946E-5	0.0	0.0	5.024704875647525	0.0
17	2.8753346889577946E-5	0.0	0.0	5.287740492993384	0.0
18	2.8753346889577946E-5	0.0	0.0	5.574325101441808	0.0
19	2.8753346889577946E-5	0.0	0.0	5.755011133295915	0.0
20	2.8753346889577946E-5	0.0	0.0	5.9466521903149525	0.0
21	5.750669377915589E-5	0.0	0.0	6.167794181242697	0.0
22	5.750669377915589E-5	0.0	0.0	6.383214256139414	0.0
23	5.750669377915589E-5	0.0	0.0	6.623132182586053	0.0
24	5.750669377915589E-5	0.0	0.0	6.798010038368466	0.0
25	5.750669377915589E-5	0.0	0.0	6.932518195117912	0.0
26	5.750669377915589E-5	0.0	2.8753346889577946E-5	7.070591766881665	0.0
27	5.750669377915589E-5	0.0	2.8753346889577946E-5	7.207428944729166	0.0
28	8.626004066873385E-5	0.0	2.8753346889577946E-5	7.368332673923245	0.0
29	8.626004066873385E-5	0.0	2.8753346889577946E-5	7.5200640854595475	0.0
30	8.626004066873385E-5	0.0	2.8753346889577946E-5	7.72291894776552	0.0
31	8.626004066873385E-5	0.0	2.8753346889577946E-5	7.917205312698398	0.0
32	8.626004066873385E-5	0.0	2.8753346889577946E-5	8.076153814303986	0.0
33	8.626004066873385E-5	0.0	2.8753346889577946E-5	8.237776377170302	0.0
34	1.1501338755831178E-4	0.0	2.8753346889577946E-5	8.418289888943074	0.0
35	1.4376673444788975E-4	0.0	2.8753346889577946E-5	8.660393069753319	0.0
36	1.4376673444788975E-4	0.0	2.8753346889577946E-5	8.862874138549728	0.0
37	1.725200813374677E-4	0.0	2.8753346889577946E-5	9.052444954592715	0.0
38	2.0127342822704562E-4	0.0	2.8753346889577946E-5	9.228185410781816	0.0
39	2.5878012200620154E-4	0.0	2.8753346889577946E-5	9.401309312403964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	165	0.0	45.000004	1
TGTAACG	125	0.0	45.000004	1
TACCCGT	45	3.8562575E-10	45.0	12
CTCCGCG	35	1.212884E-7	45.0	38
CTATCGT	40	6.8212103E-9	45.0	10
GCGCGTT	50	2.1827873E-11	45.0	23
ACGTTAG	265	0.0	45.0	1
ATAGCGC	20	7.03526E-4	45.0	32
CCGTGCA	25	3.892442E-5	45.0	12
TGTATCG	85	0.0	45.0	1
TCGATTG	110	0.0	45.0	1
CTGCGAA	45	3.8562575E-10	45.0	32
CGTAGAT	40	6.8212103E-9	45.0	36
GTACTCG	20	7.03526E-4	45.0	1
TGTTACG	195	0.0	45.0	1
AACGACA	40	6.8212103E-9	45.0	22
CCGACAA	20	7.03526E-4	45.0	12
CTATTCG	55	1.8189894E-12	45.0	25
CGTGTAT	35	1.212884E-7	45.0	18
ACCGTTG	35	1.212884E-7	45.0	10
>>END_MODULE