FastQCFastQC Report
Thu 26 May 2016
SRR1545329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545329_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3682631
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50230.136397048740425No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.035313E-445.00000432
CTACGAT351.2129021E-745.00000411
CGTACGT351.2129021E-745.00000423
CTATCGT253.8924874E-545.030
CGTAGAT453.8562575E-1045.030
TACGTAA253.8924874E-545.038
CGCATAT302.166811E-644.99999636
TGTTACG650.041.538461
ACTACGT501.0822987E-940.535
CTATACG1250.039.6000021
ACGTATG800.039.3750041
CGGGATC6300.039.2857136
CGTTTTT44900.038.8864141
TCTAGCG2550.038.823531
CGACATA356.2520394E-638.5714326
CGACACG356.2520394E-638.5714326
ACTCGAT356.2520394E-638.5714334
GTACGAT356.2520394E-638.5714311
CGGGTAT3750.038.46
TCGATAG3750.038.41
Produced by FastQC (version 0.11.5)