##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545329_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3682631 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23844854398934 31.0 31.0 33.0 30.0 34.0 2 31.555429528508288 31.0 31.0 34.0 30.0 34.0 3 31.64748762501592 31.0 31.0 34.0 30.0 34.0 4 30.275841103819523 35.0 33.0 37.0 0.0 37.0 5 32.8291501374968 35.0 33.0 37.0 19.0 37.0 6 34.442558594656916 35.0 33.0 37.0 30.0 37.0 7 35.48925863058232 36.0 35.0 37.0 33.0 37.0 8 35.67286757755529 37.0 35.0 37.0 35.0 37.0 9 37.68551695784888 39.0 37.0 39.0 35.0 39.0 10 37.0989110774335 39.0 37.0 39.0 33.0 39.0 11 36.658214195231615 39.0 35.0 39.0 32.0 39.0 12 35.66671029489515 37.0 35.0 39.0 31.0 39.0 13 35.22850863961119 37.0 35.0 39.0 30.0 39.0 14 35.89038027432018 38.0 35.0 40.0 28.0 41.0 15 36.25007501430363 38.0 35.0 40.0 30.0 41.0 16 36.43771151657606 38.0 35.0 40.0 31.0 41.0 17 36.40265723065927 38.0 35.0 40.0 31.0 41.0 18 36.362403672808924 38.0 35.0 40.0 31.0 41.0 19 36.36682306752971 38.0 35.0 40.0 31.0 41.0 20 36.25691903424481 38.0 35.0 40.0 30.0 41.0 21 36.11329834566646 37.0 34.0 40.0 30.0 41.0 22 36.04939484841137 37.0 34.0 40.0 30.0 41.0 23 36.01507237624405 37.0 34.0 40.0 30.0 41.0 24 35.95646183394427 37.0 34.0 40.0 30.0 41.0 25 35.825850322771956 37.0 34.0 40.0 30.0 41.0 26 35.68690699665538 37.0 34.0 40.0 30.0 41.0 27 35.69507724233028 37.0 34.0 40.0 30.0 41.0 28 35.701725206788296 37.0 34.0 40.0 30.0 41.0 29 35.72876538539973 37.0 34.0 40.0 30.0 41.0 30 35.65256605942871 36.0 34.0 40.0 30.0 41.0 31 35.48718810002957 36.0 34.0 40.0 29.0 41.0 32 35.437871999665454 36.0 34.0 40.0 29.0 41.0 33 35.300100390183 36.0 34.0 40.0 29.0 41.0 34 35.16771840567247 36.0 34.0 40.0 28.0 41.0 35 35.05460579677953 36.0 34.0 40.0 27.0 41.0 36 34.89214640293855 36.0 33.0 40.0 27.0 41.0 37 34.83897897997383 36.0 33.0 40.0 27.0 41.0 38 34.7920961942698 36.0 33.0 40.0 27.0 41.0 39 34.729635687094365 36.0 33.0 40.0 27.0 41.0 40 34.57048914213778 36.0 33.0 40.0 25.0 41.0 41 34.519027293258546 35.0 33.0 40.0 25.0 41.0 42 34.47637572159687 35.0 33.0 40.0 26.0 41.0 43 34.42633704001297 35.0 33.0 40.0 26.0 41.0 44 34.360070286705344 35.0 33.0 40.0 26.0 41.0 45 34.280387310050884 35.0 33.0 40.0 26.0 41.0 46 34.24419606525878 35.0 33.0 39.0 26.0 41.0 47 34.16445660724629 35.0 33.0 39.0 26.0 41.0 48 34.03656516224406 35.0 33.0 39.0 25.0 41.0 49 33.99797644673061 35.0 33.0 39.0 25.0 41.0 50 33.86203260657937 35.0 33.0 39.0 24.0 41.0 51 33.78386783796693 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 7.0 12 0.0 13 6.0 14 29.0 15 96.0 16 341.0 17 884.0 18 1916.0 19 3852.0 20 6577.0 21 10123.0 22 15640.0 23 22354.0 24 31123.0 25 41776.0 26 54251.0 27 66842.0 28 80323.0 29 97200.0 30 119451.0 31 148193.0 32 186448.0 33 264101.0 34 386901.0 35 489718.0 36 284167.0 37 360219.0 38 460636.0 39 549136.0 40 319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.51574757286299 25.03297235047443 30.180324881857562 12.270955194805017 2 28.417672039365332 26.626805672357616 30.9046711440815 14.05085114419555 3 29.759973236525735 25.87134035421958 30.45952744111479 13.909158968139899 4 22.552979106513792 38.25876119546053 25.86354701299153 13.324712685034152 5 23.949399220285713 33.01525458293269 28.526914589053316 14.508431607728278 6 22.793703740613708 41.02773261833727 25.394072878873825 10.784490762175194 7 89.81089878404869 4.013407805452135 4.339506184572932 1.8361872259262466 8 91.36486930132288 2.74939302905993 3.878585717656751 2.0071519519604326 9 87.37117566218282 4.599103195514294 5.252277515721776 2.7774436265811047 10 55.223778868966235 24.089272044904853 10.461162141957747 10.225786944171164 11 47.1967731765686 20.906221665977395 17.986678545854854 13.910326611599153 12 43.82923513107884 20.49781800022864 20.62658463473533 15.04636223395719 13 24.807345617847677 35.14723576703721 21.820024868090233 18.225393747024885 14 16.84390860773181 36.77539237572268 27.017586068221334 19.363112948324172 15 17.0882990992038 23.697839941063876 42.66419850373279 16.549662455999528 16 21.95631329883445 19.997143346699684 39.45437378873963 18.592169565726245 17 22.230953902250864 21.2840493657931 24.67328385602576 31.811712875930283 18 25.37788879743857 24.379662257771688 29.612985933154857 20.62946301163489 19 32.753675293560505 25.431030152084205 22.038455658468088 19.776838895887206 20 34.12076854835578 25.654375906790555 22.500951086329312 17.723904458524352 21 26.317054301666392 27.625032212024504 26.386787055233064 19.671126431076043 22 25.01629948805623 24.08894619091622 25.667627302328142 25.227127018699402 23 23.658112908950148 28.740647651095102 24.819646605918432 22.781592834036317 24 23.442560495471852 24.095870588174595 33.52114290027972 18.940426016073832 25 22.297753969919874 25.144604496079026 30.659520326636034 21.898121207365058 26 20.45024331788876 34.020541292353215 23.735394613253405 21.793820776504624 27 20.544931056084632 32.129474823841974 27.805093695241258 19.52050042483214 28 17.932749710736697 27.9305746353626 34.00272794097481 20.133947712925895 29 19.48210396317198 25.934393101019353 33.10820443318921 21.47529850261946 30 23.26298779323804 28.49253699325292 28.01467211892802 20.22980309458102 31 30.45474824928156 25.529085048162575 24.11113141664207 19.905035285913794 32 33.34849459530428 24.889840985969 23.152984917576592 18.60867950115013 33 30.62411085987165 25.953021087369326 22.9725432713731 20.450324781385916 34 22.044103794270995 25.717537271586536 27.999601371953908 24.238757562188553 35 22.338431409500437 25.3799253848675 28.902868628434398 23.37877457719766 36 31.121472664516208 24.901571729559656 24.67382694600681 19.303128659917327 37 23.04162431696252 31.679280384051513 26.00217073065425 19.276924568331715 38 23.121105535689022 30.643662099189413 22.978680188158954 23.25655217696261 39 22.937459658597344 29.543416106582495 25.305820756953384 22.213303477866774 40 27.743018510407367 24.26129579640208 24.53281363242747 23.46287206076308 41 19.440829124612268 22.934554127198734 26.995427996994543 30.62918875119446 42 22.156767810839586 24.151184302744426 25.715147675669918 27.97690021074607 43 22.266390523514303 24.81948367892412 26.21058151088176 26.70354428667982 44 22.143190561313368 27.451379190584124 27.726834428972115 22.678595819130397 45 19.764402135321188 32.60275058782702 22.400153585846642 25.232693691005153 46 22.507875483587686 30.351126680897433 24.54682535393853 22.59417248157635 47 21.955390045866665 25.208146023861744 28.069415589017744 24.767048341253847 48 23.088357209831774 23.51313503850915 30.71809258109216 22.68041517056691 49 22.55824707932997 23.190105117781282 31.053016172405002 23.19863163048375 50 19.93960296320755 30.418551302044648 26.242813901257012 23.399031833490785 51 19.188346592422644 31.794198224041452 24.144069823992684 24.873385359543217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 518.0 1 1328.0 2 2138.0 3 6332.5 4 10527.0 5 7746.0 6 4965.0 7 5209.0 8 5453.0 9 5888.0 10 6323.0 11 6377.0 12 6431.0 13 6380.5 14 6330.0 15 6342.0 16 6354.0 17 6641.5 18 6929.0 19 7231.0 20 7533.0 21 8601.5 22 9670.0 23 10168.0 24 10666.0 25 12187.5 26 22288.0 27 30867.0 28 36450.5 29 42034.0 30 46827.0 31 51620.0 32 61860.5 33 72101.0 34 81462.0 35 90823.0 36 94713.0 37 98603.0 38 118996.0 39 139389.0 40 188027.0 41 236665.0 42 277226.5 43 317788.0 44 318311.0 45 318834.0 46 302055.0 47 285276.0 48 281072.0 49 276868.0 50 264749.0 51 252630.0 52 234739.0 53 216848.0 54 209435.5 55 202023.0 56 201466.5 57 200910.0 58 194249.5 59 187589.0 60 176781.0 61 165973.0 62 145770.0 63 125567.0 64 108094.0 65 90621.0 66 76478.5 67 62336.0 68 54602.5 69 46869.0 70 39176.5 71 31484.0 72 25352.0 73 19220.0 74 15253.0 75 8358.0 76 5430.0 77 4333.0 78 3236.0 79 2385.5 80 1535.0 81 970.0 82 405.0 83 255.0 84 105.0 85 90.0 86 75.0 87 58.5 88 42.0 89 35.5 90 29.0 91 16.0 92 3.0 93 1.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3682631.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.537471787460937 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50514094582445 12.817390817747556 2 9.430884311569248 3.119259664667688 3 3.3721436368541022 1.6730019077322194 4 1.6780976207539071 1.1100596823929225 5 0.9815059405608314 0.811581340062503 6 0.6803387426912205 0.6750649657903625 7 0.4813049628950011 0.557169707152871 8 0.3497477670888533 0.4627155064767497 9 0.27119305352183737 0.40363627244154926 >10 2.425959968583338 9.323026225922995 >50 1.0259715282859838 12.452027351832015 >100 1.7807114797927723 54.17562503624357 >500 0.014315824487258995 1.574223197540983 >1k 0.0025052692852703244 0.6965704600755647 >5k 1.7894780609073745E-4 0.14864786392045037 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5023 0.136397048740425 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0660940506936481 0.0 2 0.0 0.0 0.0 0.2328498293747052 0.0 3 0.0 0.0 0.0 0.3437216490058331 0.0 4 0.0 0.0 0.0 0.51536523751633 0.0 5 0.0 0.0 0.0 0.888332282001645 0.0 6 0.0 0.0 0.0 1.2068002468887054 0.0 7 0.0 0.0 0.0 1.3990269456809548 0.0 8 0.0 0.0 0.0 1.814979562166288 0.0 9 0.0 0.0 0.0 1.9508335209256642 0.0 10 0.0 0.0 0.0 2.171545289223927 0.0 11 0.0 0.0 0.0 2.5305820756953383 0.0 12 0.0 0.0 0.0 2.7971306383941266 0.0 13 2.7154499052443756E-5 0.0 0.0 2.911586851900177 0.0 14 2.7154499052443756E-5 0.0 0.0 2.9635877175856065 0.0 15 2.7154499052443756E-5 0.0 0.0 3.0457572317183015 0.0 16 2.7154499052443756E-5 0.0 0.0 3.218541309189001 0.0 17 2.7154499052443756E-5 0.0 0.0 3.404739709191608 0.0 18 2.7154499052443756E-5 0.0 0.0 3.6060360106673732 0.0 19 2.7154499052443756E-5 0.0 0.0 3.744062329350945 0.0 20 5.430899810488751E-5 0.0 0.0 3.895394352570214 0.0 21 8.146349715733127E-5 0.0 0.0 4.091992925709907 0.0 22 8.146349715733127E-5 0.0 0.0 4.301408422402353 0.0 23 8.146349715733127E-5 0.0 0.0 4.511991562554054 0.0 24 8.146349715733127E-5 0.0 0.0 4.676656444808073 0.0 25 8.146349715733127E-5 0.0 0.0 4.819842118311609 0.0 26 8.146349715733127E-5 0.0 0.0 4.962701937826516 0.0 27 8.146349715733127E-5 0.0 0.0 5.134101135845541 0.0 28 1.0861799620977503E-4 0.0 0.0 5.287361128497533 0.0 29 1.0861799620977503E-4 0.0 0.0 5.470871233093948 0.0 30 1.0861799620977503E-4 0.0 0.0 5.669859402150256 0.0 31 1.0861799620977503E-4 0.0 0.0 5.849730803873643 0.0 32 1.0861799620977503E-4 0.0 0.0 6.006466572404349 0.0 33 1.3577249526221878E-4 0.0 0.0 6.18071699282388 0.0 34 1.6292699431466254E-4 0.0 0.0 6.376365158496737 0.0 35 1.6292699431466254E-4 0.0 0.0 6.600335466681294 0.0 36 1.6292699431466254E-4 0.0 0.0 6.790172569556928 0.0 37 1.900814933671063E-4 0.0 0.0 6.996356680862133 0.0 38 2.1723599241955005E-4 0.0 0.0 7.252722306416255 0.0 39 2.1723599241955005E-4 0.0 0.0 7.746771262176416 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGAAC 20 7.035313E-4 45.000004 32 CTACGAT 35 1.2129021E-7 45.000004 11 CGTACGT 35 1.2129021E-7 45.000004 23 CTATCGT 25 3.8924874E-5 45.0 30 CGTAGAT 45 3.8562575E-10 45.0 30 TACGTAA 25 3.8924874E-5 45.0 38 CGCATAT 30 2.166811E-6 44.999996 36 TGTTACG 65 0.0 41.53846 1 ACTACGT 50 1.0822987E-9 40.5 35 CTATACG 125 0.0 39.600002 1 ACGTATG 80 0.0 39.375004 1 CGGGATC 630 0.0 39.285713 6 CGTTTTT 4490 0.0 38.886414 1 TCTAGCG 255 0.0 38.82353 1 CGACATA 35 6.2520394E-6 38.57143 26 CGACACG 35 6.2520394E-6 38.57143 26 ACTCGAT 35 6.2520394E-6 38.57143 34 GTACGAT 35 6.2520394E-6 38.57143 11 CGGGTAT 375 0.0 38.4 6 TCGATAG 375 0.0 38.4 1 >>END_MODULE