FastQCFastQC Report
Thu 26 May 2016
SRR1545328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545328_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2764412
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28960.10476007194296652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA351.2127566E-745.014
CACGGTA207.035009E-445.042
TATATCG207.035009E-445.043
CCGTCGA207.035009E-445.010
ATCCGGT253.8922335E-545.019
TCCCGTA750.045.043
CGTCGAT453.8562575E-1045.019
ATCGGTC207.035009E-445.026
GGTACGA302.1666128E-644.99999641
ACGTTAC302.1666128E-644.99999645
ATGGTCG302.1666128E-644.99999644
TCACGAC3250.041.5384625
CGTTTTT28500.041.2894741
CGGGATC5450.041.2844056
GGCGATA7400.041.04738
CGACGGT3250.040.8461528
ACGGGTC2100.040.7142875
AACGTCC501.0822987E-940.519
GTAATCG501.0822987E-940.51
CGTTAGG4500.040.52
Produced by FastQC (version 0.11.5)