##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545328_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2764412
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24954203642583	31.0	31.0	33.0	30.0	34.0
2	31.56661995389978	31.0	31.0	34.0	30.0	34.0
3	31.67099983649326	31.0	31.0	34.0	30.0	34.0
4	30.288178462544657	35.0	33.0	37.0	0.0	37.0
5	32.84415058247468	35.0	33.0	37.0	19.0	37.0
6	34.47457506334077	35.0	33.0	37.0	30.0	37.0
7	35.5103121387116	36.0	35.0	37.0	33.0	37.0
8	35.69364515853643	37.0	35.0	37.0	35.0	37.0
9	37.71533801763268	39.0	37.0	39.0	35.0	39.0
10	37.067760521948244	39.0	37.0	39.0	33.0	39.0
11	36.65634246993574	39.0	35.0	39.0	32.0	39.0
12	35.69964643475719	37.0	35.0	39.0	31.0	39.0
13	35.30475486287862	37.0	35.0	39.0	30.0	39.0
14	36.117897042843104	38.0	35.0	40.0	30.0	41.0
15	36.42336417292357	38.0	35.0	40.0	31.0	41.0
16	36.59674136850802	38.0	35.0	40.0	32.0	41.0
17	36.5369836334092	38.0	35.0	40.0	31.0	41.0
18	36.52947353722962	38.0	35.0	40.0	31.0	41.0
19	36.51792750139994	38.0	35.0	40.0	31.0	41.0
20	36.418579430273056	38.0	35.0	40.0	31.0	41.0
21	36.26305087664212	38.0	35.0	40.0	31.0	41.0
22	36.12822690684312	37.0	34.0	40.0	30.0	41.0
23	36.091850635867594	37.0	34.0	40.0	30.0	41.0
24	36.03272486156188	37.0	34.0	40.0	30.0	41.0
25	35.89788931606432	37.0	34.0	40.0	30.0	41.0
26	35.75973335378374	37.0	34.0	40.0	30.0	41.0
27	35.76186545276175	37.0	34.0	40.0	30.0	41.0
28	35.7778836873809	37.0	34.0	40.0	30.0	41.0
29	35.828514707648495	37.0	34.0	40.0	30.0	41.0
30	35.784094773138015	37.0	34.0	40.0	30.0	41.0
31	35.68750895307935	37.0	34.0	40.0	30.0	41.0
32	35.598722259923626	37.0	34.0	40.0	30.0	41.0
33	35.48602017354866	37.0	34.0	40.0	29.0	41.0
34	35.40727467541018	36.0	34.0	40.0	29.0	41.0
35	35.30933413687974	36.0	34.0	40.0	29.0	41.0
36	35.14011117011502	36.0	34.0	40.0	27.0	41.0
37	35.11160456545551	36.0	34.0	40.0	27.0	41.0
38	35.0791090474213	36.0	34.0	40.0	28.0	41.0
39	35.01222212897354	36.0	34.0	40.0	27.0	41.0
40	34.938514230150936	36.0	34.0	40.0	27.0	41.0
41	34.88107416694762	36.0	34.0	40.0	27.0	41.0
42	34.803537967567785	36.0	33.0	40.0	27.0	41.0
43	34.772357014800974	35.0	33.0	40.0	27.0	41.0
44	34.69245032940097	35.0	33.0	40.0	27.0	41.0
45	34.585201482268204	35.0	33.0	40.0	27.0	41.0
46	34.54775843832251	35.0	33.0	40.0	27.0	41.0
47	34.48151252418236	35.0	33.0	40.0	27.0	41.0
48	34.36976615641952	35.0	33.0	39.0	26.0	41.0
49	34.33581427081057	35.0	33.0	39.0	26.0	41.0
50	34.20057646978815	35.0	33.0	39.0	26.0	41.0
51	34.11848523302605	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	18.0
15	53.0
16	196.0
17	484.0
18	1177.0
19	2238.0
20	4054.0
21	6539.0
22	9969.0
23	14430.0
24	20597.0
25	28403.0
26	37318.0
27	46652.0
28	56088.0
29	68907.0
30	85798.0
31	106302.0
32	136095.0
33	195577.0
34	288323.0
35	383760.0
36	208790.0
37	267632.0
38	353159.0
39	441587.0
40	258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52275709988236	25.808634892338766	30.336071468362892	11.332536539415978
2	27.721736123269615	27.076535624935794	31.620503745461964	13.581224506332632
3	29.098701640710573	26.023074708111526	30.855892681698677	14.022330969479224
4	22.528877750494498	38.99491103352178	25.76269383868975	12.713517377293979
5	23.6035366653017	32.61416894442652	29.44369363177414	14.338600758497646
6	22.347320153435884	42.357108853528345	24.4647686379599	10.830802355075871
7	91.00376499595573	3.6874026013488583	3.763440471246688	1.545391931448713
8	92.66310521007722	2.3459600088554096	3.3337650104253638	1.6571697706420025
9	88.65867316449211	4.49853350368903	4.560897579666128	2.2818957521527183
10	53.662659545682764	24.652692869224992	11.218479734569232	10.466167850523005
11	46.52544555587228	21.128543791591124	18.540072897961664	13.805937754574932
12	42.57227215046093	22.468684117996883	19.731646368196927	15.22739736334526
13	23.152482336207484	38.06379078082428	20.867909703763406	17.915817179204836
14	16.593040400634926	39.59286821211889	25.39194591833634	18.422145468909846
15	16.49244034536097	24.856425163832306	42.20380319576098	16.447331295045746
16	19.193810473981447	21.020781272834874	40.954170362449595	18.831237890734087
17	19.125730896841716	21.847647890401287	25.7779592911621	33.2486619215949
18	23.850388437034713	25.378416820647576	29.64529165695996	21.125903085357752
19	31.005074496855027	26.850049847851913	21.808724604002588	20.336151051290475
20	33.38858317790546	25.894837672532166	22.653569728390703	18.063009421171664
21	25.52781568015187	28.743653261525417	26.05526961972383	19.673261438598878
22	24.528145587560754	24.864564326880366	24.581429974982022	26.025860110576858
23	22.915397560132135	29.6129158750577	24.458799918391325	23.01288664641884
24	22.52352398991178	25.007632726236178	34.33959916249821	18.129244121353835
25	20.50338372138451	26.737078264744905	31.811032508902436	20.948505504968146
26	19.233999852409845	36.04205885374539	23.49450805451575	21.229433239329015
27	20.36697134869911	34.06652119872147	27.31289691985131	18.253610532728118
28	17.53439791174398	29.16819200611197	35.5620291042001	17.735380977943954
29	18.04372864826227	26.22246611575988	34.53309419869397	21.200711037283877
30	20.21684900803498	30.8486940441584	28.766623788349925	20.167833159456695
31	29.999905947449225	27.455820622975157	22.307347819355435	20.236925610220187
32	31.568991886882277	26.888611393670697	23.52163859800927	18.020758121437762
33	28.032579803589336	28.046542989974	23.672846160413137	20.24803104602353
34	20.176153192794708	27.6535841980139	26.895592986863033	25.274669622328368
35	21.228962976575126	25.935895228352358	28.93552046511157	23.899621329960947
36	29.112339260573318	25.84527921308401	25.427215624877913	19.61516590146476
37	21.989558719901375	31.818520538906647	26.586811227848816	19.605109513343162
38	21.32905659503721	32.88587229399959	22.733948485247495	23.051122625715703
39	21.568673555171948	31.23297106220057	25.560444680460076	21.637910702167403
40	26.05993607320472	25.212775809105153	24.783245044515795	23.94404307317433
41	19.063113602458678	24.503294009720694	26.54506636492679	29.888526022893835
42	21.563464490821193	26.290581866957602	24.409530851407098	27.736422790814103
43	22.582017441683803	26.816407973919954	25.03870624205075	25.562868342345496
44	21.59348895895402	29.13512891710787	27.01131379837738	22.260068325560734
45	19.511454877203544	34.10924276120926	22.3200449137104	24.0592574478768
46	22.176904166238607	32.74038023275836	23.92020436895803	21.162511232045006
47	22.017014829916814	27.110792457853606	26.345240868582543	24.52695184364704
48	22.858423418795752	24.051335329176695	30.429400537980587	22.660840714046966
49	21.54523276559355	24.270694816836276	30.67719283522138	23.506879582348798
50	19.581958116228694	31.857986436175217	26.09889553366141	22.46115991393468
51	18.816334178841647	33.727425579110495	23.45475276478325	24.001487477264604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	415.0
1	989.5
2	1564.0
3	4049.5
4	6535.0
5	5014.0
6	3493.0
7	3716.5
8	3940.0
9	4227.0
10	4514.0
11	4661.5
12	4809.0
13	4683.0
14	4557.0
15	4362.0
16	4167.0
17	4142.5
18	4118.0
19	4962.5
20	5807.0
21	5949.5
22	6092.0
23	7598.0
24	9104.0
25	11538.0
26	18061.0
27	22150.0
28	27975.5
29	33801.0
30	38409.5
31	43018.0
32	51535.0
33	60052.0
34	65205.0
35	70358.0
36	73836.0
37	77314.0
38	93751.5
39	110189.0
40	151354.0
41	192519.0
42	239159.5
43	285800.0
44	279749.5
45	273699.0
46	259398.5
47	245098.0
48	232572.0
49	220046.0
50	206554.5
51	193063.0
52	177068.0
53	161073.0
54	147979.5
55	134886.0
56	132484.5
57	130083.0
58	124336.0
59	118589.0
60	110297.0
61	102005.0
62	88057.0
63	74109.0
64	63180.0
65	52251.0
66	43087.5
67	33924.0
68	28375.5
69	22827.0
70	19639.5
71	16452.0
72	12358.0
73	8264.0
74	6554.0
75	3751.5
76	2659.0
77	1986.0
78	1313.0
79	912.0
80	511.0
81	408.0
82	305.0
83	198.5
84	92.0
85	53.0
86	14.0
87	12.5
88	11.0
89	7.0
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2764412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.59920903074361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92612350869109	12.93512013076307
2	9.171850018996432	3.0449091132790302
3	3.214156210487241	1.600573523860214
4	1.588340713911088	1.0546079808900275
5	0.8872554013506109	0.7363868935337553
6	0.5758766051875881	0.5735457687254271
7	0.3916858379917221	0.4551172569444599
8	0.2941779109953668	0.3906496509471666
9	0.23279042957668497	0.3477723300809994
>10	2.63459056094449	10.652423109095183
>50	1.2828074117172814	15.593834427943676
>100	1.7872632133532307	50.79507418187327
>500	0.010465741437779138	1.1171575290533629
>1k	0.0026164353594447846	0.7028281030103873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2896	0.10476007194296652	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.057191185684333595	0.0
2	0.0	0.0	0.0	0.20250237663561002	0.0
3	0.0	0.0	0.0	0.29293752161399966	0.0
4	0.0	0.0	0.0	0.43976802300091306	0.0
5	0.0	0.0	0.0	0.7594743475285161	0.0
6	0.0	0.0	0.0	1.044308880152452	0.0
7	0.0	0.0	0.0	1.1989529780654982	0.0
8	0.0	0.0	0.0	1.5714734272604807	0.0
9	0.0	0.0	0.0	1.6816234338441594	0.0
10	0.0	0.0	0.0	1.8866579945391642	0.0
11	0.0	0.0	0.0	2.2128756495052113	0.0
12	0.0	0.0	0.0	2.4442087503599317	0.0
13	0.0	0.0	0.0	2.5503072624485785	0.0
14	0.0	0.0	0.0	2.593643783922223	0.0
15	0.0	0.0	0.0	2.666425988600831	0.0
16	0.0	0.0	0.0	2.836407887102212	0.0
17	0.0	0.0	0.0	3.0383314788099605	0.0
18	0.0	0.0	0.0	3.239386893125916	0.0
19	0.0	0.0	0.0	3.3795251937844286	0.0
20	0.0	0.0	0.0	3.513839471106333	0.0
21	0.0	0.0	0.0	3.7036809274449682	0.0
22	0.0	0.0	0.0	3.907847310748181	0.0
23	0.0	0.0	0.0	4.124891658696316	0.0
24	0.0	0.0	0.0	4.298273918648884	0.0
25	3.617405799135585E-5	0.0	0.0	4.442101973222515	0.0
26	7.23481159827117E-5	0.0	0.0	4.580757137503382	0.0
27	7.23481159827117E-5	0.0	0.0	4.725670413816754	0.0
28	7.23481159827117E-5	0.0	0.0	4.874309618103235	0.0
29	7.23481159827117E-5	0.0	0.0	5.038431319210017	0.0
30	7.23481159827117E-5	0.0	0.0	5.228345123664635	0.0
31	7.23481159827117E-5	0.0	0.0	5.405995922460183	0.0
32	7.23481159827117E-5	0.0	0.0	5.5706602344368354	0.0
33	7.23481159827117E-5	0.0	0.0	5.740244218300311	0.0
34	7.23481159827117E-5	0.0	0.0	5.916882143472102	0.0
35	7.23481159827117E-5	0.0	0.0	6.137724767509329	0.0
36	1.0852217397406754E-4	0.0	0.0	6.333426421242565	0.0
37	1.446962319654234E-4	0.0	0.0	6.531189996281307	0.0
38	1.446962319654234E-4	0.0	0.0	6.7455574639380815	0.0
39	1.8087028995677923E-4	0.0	0.0	7.020154738150464	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	35	1.2127566E-7	45.0	14
CACGGTA	20	7.035009E-4	45.0	42
TATATCG	20	7.035009E-4	45.0	43
CCGTCGA	20	7.035009E-4	45.0	10
ATCCGGT	25	3.8922335E-5	45.0	19
TCCCGTA	75	0.0	45.0	43
CGTCGAT	45	3.8562575E-10	45.0	19
ATCGGTC	20	7.035009E-4	45.0	26
GGTACGA	30	2.1666128E-6	44.999996	41
ACGTTAC	30	2.1666128E-6	44.999996	45
ATGGTCG	30	2.1666128E-6	44.999996	44
TCACGAC	325	0.0	41.53846	25
CGTTTTT	2850	0.0	41.289474	1
CGGGATC	545	0.0	41.284405	6
GGCGATA	740	0.0	41.0473	8
CGACGGT	325	0.0	40.84615	28
ACGGGTC	210	0.0	40.714287	5
AACGTCC	50	1.0822987E-9	40.5	19
GTAATCG	50	1.0822987E-9	40.5	1
CGTTAGG	450	0.0	40.5	2
>>END_MODULE