SRR1545327_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545327_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3158754
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

$Duplication level graph$

Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4081	0.129196512295671	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT	3701	0.11716645234165117	TruSeq Adapter, Index 16 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCT	3471	0.1098851002642181	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3433	0.10868209426881613	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	3214	0.10174898076899942	TruSeq Adapter, Index 13 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCTCG	45	3.8562575E-10	45.000004	10
CTAAGCG	135	0.0	45.000004	1
ATCGTTC	90	0.0	45.000004	45
CGACTTA	45	3.8562575E-10	45.000004	31
CGACTGG	85	0.0	45.000004	2
TGTACCG	45	3.8562575E-10	45.000004	23
TATCACT	25	3.89236E-5	45.0	38
CTATGCG	165	0.0	45.0	1
TACCCCG	40	6.8212103E-9	45.0	42
TCCGCGT	20	7.0351636E-4	45.0	14
ACGTTCC	20	7.0351636E-4	45.0	20
CTATCGT	25	3.89236E-5	45.0	12
ACGTTAG	220	0.0	45.0	1
CCTACTA	75	0.0	45.0	40
GCGCCTA	20	7.0351636E-4	45.0	33
ATCCGTT	20	7.0351636E-4	45.0	32
ATGTACG	80	0.0	45.0	1
CGCCTAT	20	7.0351636E-4	45.0	34
CGCCTAG	25	3.89236E-5	45.0	32
ATTCCCG	25	3.89236E-5	45.0	32