##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545327_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3158754
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26637718543451	31.0	31.0	33.0	30.0	34.0
2	31.55935853187681	31.0	31.0	34.0	30.0	34.0
3	31.63602547080273	31.0	31.0	34.0	30.0	34.0
4	30.23358197567775	35.0	33.0	37.0	0.0	37.0
5	32.78989943503039	35.0	33.0	37.0	19.0	37.0
6	34.39835264157956	35.0	33.0	37.0	30.0	37.0
7	35.47847474035648	36.0	35.0	37.0	33.0	37.0
8	35.67739906304828	37.0	35.0	37.0	35.0	37.0
9	37.71523360160367	39.0	37.0	39.0	35.0	39.0
10	37.193652623787735	39.0	37.0	39.0	33.0	39.0
11	36.637486806506615	39.0	35.0	39.0	32.0	39.0
12	34.99168279644442	35.0	34.0	39.0	31.0	39.0
13	34.30388248024379	35.0	33.0	39.0	26.0	39.0
14	34.96336245241003	35.0	33.0	40.0	27.0	41.0
15	35.47620169218622	35.0	33.0	40.0	30.0	41.0
16	35.798277422046795	35.0	34.0	40.0	31.0	41.0
17	35.74512798400888	35.0	34.0	40.0	31.0	41.0
18	35.75921708369819	36.0	34.0	40.0	31.0	41.0
19	35.7054639265989	36.0	34.0	40.0	31.0	41.0
20	35.53925820117679	35.0	34.0	40.0	31.0	41.0
21	35.30664401216429	35.0	34.0	40.0	30.0	41.0
22	35.14835659883612	35.0	34.0	40.0	30.0	41.0
23	35.173983792343435	35.0	34.0	39.0	30.0	41.0
24	35.07900995139222	35.0	34.0	39.0	30.0	41.0
25	34.9735797722773	35.0	34.0	39.0	29.0	41.0
26	34.809161777080455	35.0	34.0	39.0	29.0	41.0
27	34.77635548700532	35.0	34.0	39.0	29.0	41.0
28	34.87506434499173	35.0	34.0	39.0	29.0	41.0
29	34.989635153608035	35.0	34.0	39.0	29.0	41.0
30	34.93246134393498	35.0	34.0	39.0	29.0	41.0
31	34.713533564183855	35.0	34.0	39.0	29.0	41.0
32	34.54538941620652	35.0	34.0	39.0	29.0	41.0
33	34.41858466977802	35.0	33.0	39.0	28.0	41.0
34	34.37405033756981	35.0	34.0	39.0	27.0	41.0
35	34.27026289479966	35.0	33.0	39.0	27.0	41.0
36	33.998018839073886	35.0	33.0	38.0	25.0	41.0
37	33.90968400831467	35.0	33.0	38.0	25.0	41.0
38	33.930229767813515	35.0	33.0	38.0	26.0	41.0
39	33.918492544845215	35.0	33.0	38.0	25.0	41.0
40	33.78044127526233	35.0	33.0	38.0	24.0	41.0
41	33.83295976831371	35.0	33.0	38.0	25.0	41.0
42	33.774853628994215	35.0	33.0	38.0	25.0	41.0
43	33.634499869252245	35.0	33.0	38.0	25.0	41.0
44	33.558361936383776	35.0	33.0	38.0	24.0	40.0
45	33.47274938155994	35.0	33.0	38.0	24.0	40.0
46	33.422396299300296	35.0	33.0	38.0	24.0	40.0
47	33.375146655928255	35.0	33.0	38.0	24.0	40.0
48	33.312756548943035	35.0	33.0	37.0	23.0	40.0
49	33.351197339204	35.0	33.0	37.0	24.0	40.0
50	33.12957102705687	35.0	32.0	37.0	24.0	40.0
51	33.017722494375946	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	7.0
13	3.0
14	11.0
15	84.0
16	289.0
17	766.0
18	1765.0
19	3564.0
20	6014.0
21	9899.0
22	15078.0
23	21982.0
24	30403.0
25	41179.0
26	52197.0
27	63969.0
28	76084.0
29	91350.0
30	112442.0
31	140351.0
32	181066.0
33	271909.0
34	424809.0
35	609078.0
36	189023.0
37	218599.0
38	272754.0
39	323799.0
40	278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.422548257952343	26.576491869895534	30.735600176525303	11.26535969562682
2	27.5376303441167	27.63785973836519	31.73577302949201	13.088736888026101
3	29.227188948553767	25.46504096235414	31.27267903736727	14.035091051724827
4	22.72443501456587	39.12754839408197	25.83198944900426	12.3160271423479
5	24.421686525763008	32.49927028188963	28.826651268189924	14.252391924157436
6	21.987625500434664	40.804380461409785	25.242516511257286	11.965477526898265
7	88.28829342202653	4.84165591875784	4.902281089315597	1.9677695699000302
8	90.47963215875627	2.868979350718669	3.857913595044122	2.79347489548094
9	87.84194653967988	4.285772174724591	5.464464785798451	2.4078164997970717
10	68.30668041892469	15.707902546383796	7.702847388558906	8.28256964613262
11	62.20636364845126	15.463312432687065	13.19922349128802	9.131100427573656
12	53.73561853819576	20.744888649131905	14.918319058717454	10.601173753954882
13	18.777182395336894	54.3067614635391	15.916877351006125	10.999178790117876
14	11.407947564134465	53.50565444475891	23.101767342439455	11.984630648667165
15	10.218142976629393	21.225806124820103	57.302562972615156	11.253487925935353
16	11.915774384456657	15.025988095305934	54.41807117616629	18.640166344071112
17	13.089528339338866	17.49591769412876	23.917532039532045	45.49702192700033
18	21.87793034848551	23.44224969719073	34.896766256568256	19.783053697755506
19	34.61026088134752	23.868462058140647	20.308260788906004	21.21301627160583
20	37.72091780493195	23.55675687312149	19.748166523888848	18.974158798057715
21	21.525892804567878	32.08360005242574	25.037372330988738	21.35313481201765
22	23.028004080089808	24.859359101721754	21.929184735500137	30.1834520826883
23	18.753628804269027	34.515983200971014	20.211956993168826	26.518431001591136
24	20.065348551992336	22.365780937673527	42.46357899348921	15.105291516844932
25	14.726439602450839	24.699106039913207	38.67138751545704	21.90306684217891
26	14.50315535809373	42.20502767863531	23.2225111547148	20.06930580855616
27	16.88634189303757	39.1566738023917	26.759095516776554	17.197888787794174
28	12.609497289120963	29.832205990083434	43.099969165056855	14.458327555738752
29	13.523085368471238	24.75520410896195	40.95839688687375	20.763313635693063
30	18.86392545921588	33.97573853487799	29.67875940956466	17.48157659634147
31	35.7061360270537	26.39990325299153	19.916239124667513	17.977721595287257
32	36.785327379086816	26.534260027846422	22.250292362114934	14.430120230951824
33	31.59907355875133	28.034693426585296	20.649281330549957	19.716951684113422
34	16.971881950921155	28.356022659567664	26.678082560401982	27.9940128291092
35	19.116651692407828	24.82789099752624	31.562825088626717	24.492632221439212
36	36.24457618415363	23.030251801818057	23.10467988327043	17.620492130757885
37	19.72242852719775	34.48825074697175	28.450015417471576	17.33930530835893
38	19.441621601428917	37.83210088534909	19.988071245814016	22.73820626740797
39	20.031885990488654	34.39419467296282	25.732678138278576	19.84124119826995
40	26.905988880425635	25.107748181719753	20.965355326815573	27.020907611039036
41	15.696252383059903	22.11049040222822	25.387320443440675	36.805936771271206
42	22.87221480368525	24.044607462309507	20.818810201744107	32.26436753226114
43	22.729848541545177	24.842358727523575	23.97758736514461	28.450205365786633
44	18.717728572722027	32.03750592797034	27.871242901473174	21.373522597834462
45	15.922639116563051	42.57089979150007	18.824036313052552	22.682424778884332
46	23.042186887614548	35.40861364955929	22.886777507840115	18.662421954986048
47	21.891258388592462	25.703584388021355	25.126806329331124	27.27835089405506
48	23.31105872758689	20.449898915838332	33.657891687671786	22.581150668902993
49	21.239893958187313	20.388830532545427	34.98645351933072	23.384821989936537
50	18.338306813382747	36.109301325775924	24.867052008481824	20.685339852359505
51	16.73526966645709	38.41495729012136	20.510745692763667	24.339027350657886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1070.0
1	1377.0
2	1684.0
3	4207.5
4	6731.0
5	5191.0
6	3651.0
7	4441.0
8	5231.0
9	6229.0
10	7227.0
11	7847.5
12	8468.0
13	8256.5
14	8045.0
15	8092.5
16	8140.0
17	7950.0
18	7760.0
19	8017.5
20	8275.0
21	7840.0
22	7405.0
23	7935.0
24	8465.0
25	9184.5
26	12992.5
27	16081.0
28	19510.0
29	22939.0
30	27381.0
31	31823.0
32	39241.0
33	46659.0
34	49108.5
35	51558.0
36	52405.0
37	53252.0
38	81858.0
39	110464.0
40	200252.0
41	290040.0
42	353688.5
43	417337.0
44	410276.0
45	403215.0
46	375323.0
47	347431.0
48	314805.0
49	282179.0
50	259979.0
51	237779.0
52	203631.5
53	169484.0
54	151210.5
55	132937.0
56	119164.5
57	105392.0
58	95932.5
59	86473.0
60	82894.0
61	79315.0
62	69046.5
63	58778.0
64	49603.0
65	40428.0
66	35185.0
67	29942.0
68	23073.0
69	16204.0
70	15410.5
71	14617.0
72	12741.0
73	10865.0
74	8112.0
75	4371.0
76	3383.0
77	2490.5
78	1598.0
79	1169.0
80	740.0
81	466.0
82	192.0
83	156.5
84	121.0
85	112.5
86	104.0
87	54.0
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3158754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.47570999458162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25187973952609	11.038000976510936
2	10.65120351794437	3.083674664380605
3	4.114079724120545	1.786626749428721
4	2.038543649973242	1.1803746675323423
5	1.1934341289001327	0.8637903173797229
6	0.7541785103394679	0.6550361639891827
7	0.4917016056534454	0.4982410893116587
8	0.35194105524733044	0.4075677320757906
9	0.2621618359260561	0.34154808376614093
>10	1.6741844690457193	5.1193265010962286
>50	0.5039966451618567	5.356820774494422
>100	1.5897834985261434	57.1099089012042
>500	0.11128484480522854	9.880822550911708
>1k	0.011626774830397011	2.6782608279183555
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4081	0.129196512295671	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT	3701	0.11716645234165117	TruSeq Adapter, Index 16 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCT	3471	0.1098851002642181	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3433	0.10868209426881613	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	3214	0.10174898076899942	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1364145482680829	0.0
2	0.0	0.0	0.0	0.4546412921044184	0.0
3	0.0	0.0	0.0	0.6414238019168318	0.0
4	0.0	0.0	0.0	1.0875490778959045	0.0
5	0.0	0.0	0.0	1.6893686561220025	0.0
6	0.0	0.0	0.0	2.3564671386249136	0.0
7	0.0	0.0	0.0	2.6535463033841826	0.0
8	0.0	0.0	0.0	3.3683534710205354	0.0
9	0.0	0.0	0.0	3.557288728403668	0.0
10	0.0	0.0	0.0	3.845187057934869	0.0
11	0.0	0.0	0.0	4.319931213383505	0.0
12	3.165805251057854E-5	0.0	0.0	4.679155135221039	0.0
13	3.165805251057854E-5	0.0	0.0	4.837097159196316	0.0
14	3.165805251057854E-5	0.0	0.0	4.899748445114751	0.0
15	3.165805251057854E-5	0.0	0.0	5.002542141616599	0.0
16	3.165805251057854E-5	0.0	0.0	5.2153159125401976	0.0
17	3.165805251057854E-5	0.0	0.0	5.454080944574981	0.0
18	3.165805251057854E-5	0.0	0.0	5.73526776697394	0.0
19	3.165805251057854E-5	0.0	0.0	5.90052280107916	0.0
20	3.165805251057854E-5	0.0	0.0	6.0521648726048305	0.0
21	3.165805251057854E-5	0.0	0.0	6.275480775014452	0.0
22	3.165805251057854E-5	0.0	0.0	6.502627301777853	0.0
23	3.165805251057854E-5	0.0	0.0	6.744051610223525	0.0
24	3.165805251057854E-5	0.0	0.0	6.91288400426244	0.0
25	3.165805251057854E-5	0.0	0.0	7.044518186601426	0.0
26	3.165805251057854E-5	0.0	0.0	7.182832218020144	0.0
27	3.165805251057854E-5	0.0	0.0	7.2988589804714135	0.0
28	3.165805251057854E-5	0.0	0.0	7.437014721627579	0.0
29	3.165805251057854E-5	0.0	0.0	7.579254351557608	0.0
30	3.165805251057854E-5	0.0	0.0	7.791394961430995	0.0
31	6.331610502115708E-5	0.0	0.0	7.986756803473774	0.0
32	9.497415753173561E-5	0.0	0.0	8.143939034188797	0.0
33	9.497415753173561E-5	0.0	0.0	8.299221781753184	0.0
34	1.2663221004231417E-4	0.0	0.0	8.472486303143581	0.0
35	1.5829026255289268E-4	0.0	0.0	8.704033299205953	0.0
36	1.5829026255289268E-4	0.0	0.0	8.890815809018367	0.0
37	1.8994831506347122E-4	0.0	0.0	9.071678263011302	0.0
38	2.2160636757404976E-4	0.0	0.0	9.243328223723658	0.0
39	2.2160636757404976E-4	0.0	0.0	9.427673063492756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCTCG	45	3.8562575E-10	45.000004	10
CTAAGCG	135	0.0	45.000004	1
ATCGTTC	90	0.0	45.000004	45
CGACTTA	45	3.8562575E-10	45.000004	31
CGACTGG	85	0.0	45.000004	2
TGTACCG	45	3.8562575E-10	45.000004	23
TATCACT	25	3.89236E-5	45.0	38
CTATGCG	165	0.0	45.0	1
TACCCCG	40	6.8212103E-9	45.0	42
TCCGCGT	20	7.0351636E-4	45.0	14
ACGTTCC	20	7.0351636E-4	45.0	20
CTATCGT	25	3.89236E-5	45.0	12
ACGTTAG	220	0.0	45.0	1
CCTACTA	75	0.0	45.0	40
GCGCCTA	20	7.0351636E-4	45.0	33
ATCCGTT	20	7.0351636E-4	45.0	32
ATGTACG	80	0.0	45.0	1
CGCCTAT	20	7.0351636E-4	45.0	34
CGCCTAG	25	3.89236E-5	45.0	32
ATTCCCG	25	3.89236E-5	45.0	32
>>END_MODULE