FastQCFastQC Report
Thu 26 May 2016
SRR1545326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545326_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2394494
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33860.14140774627123726No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGC406.8212103E-945.023
CGTTGTA207.0348196E-445.019
TATGCGA207.0348196E-445.040
TACGCGA406.8212103E-945.037
ACCGTAG207.0348196E-445.01
TAGTGAT406.8212103E-945.024
AGCTAAC406.8212103E-945.023
ACTCACG207.0348196E-445.01
TAAGCTT406.8212103E-945.01
CGAAGCA207.0348196E-445.043
CGGAATA453.8562575E-1044.99999623
AACGTAG551.8189894E-1244.9999961
TCCGCTA302.166491E-644.99999614
CTCCGTA253.8920778E-544.99999637
GGTCGAC253.8920778E-544.99999613
CACATCG453.8562575E-1044.99999619
GCACGAC253.8920778E-544.99999635
ACTTACG600.044.9999961
ATATCGT551.8189894E-1244.99999629
CCGTAGA302.166491E-644.99999619
Produced by FastQC (version 0.11.5)