##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545326_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2394494
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297169255801016	31.0	31.0	33.0	30.0	34.0
2	31.61225043787957	31.0	31.0	34.0	30.0	34.0
3	31.706446121811123	31.0	31.0	34.0	30.0	34.0
4	30.2884772315153	35.0	33.0	37.0	0.0	37.0
5	32.87384599836124	35.0	33.0	37.0	19.0	37.0
6	34.49871580383998	35.0	33.0	37.0	30.0	37.0
7	35.49618290962516	36.0	35.0	37.0	33.0	37.0
8	35.68613661174344	37.0	35.0	37.0	35.0	37.0
9	37.734346379652656	39.0	37.0	39.0	35.0	39.0
10	37.212168416375235	39.0	37.0	39.0	34.0	39.0
11	36.733165336810195	39.0	35.0	39.0	32.0	39.0
12	35.380441963938935	35.0	35.0	39.0	31.0	39.0
13	34.8327976599649	35.0	34.0	39.0	30.0	39.0
14	35.5822942133077	36.0	34.0	40.0	29.0	41.0
15	35.9327665886822	36.0	34.0	40.0	31.0	41.0
16	36.17167343079582	36.0	35.0	40.0	31.0	41.0
17	36.09962397065935	36.0	34.0	40.0	31.0	41.0
18	36.11854529599991	36.0	35.0	40.0	31.0	41.0
19	36.10472651006852	36.0	35.0	40.0	31.0	41.0
20	35.996369170271464	36.0	34.0	40.0	31.0	41.0
21	35.80834197120561	35.0	34.0	40.0	31.0	41.0
22	35.66090497616616	35.0	34.0	40.0	30.0	41.0
23	35.64851906081201	35.0	34.0	40.0	30.0	41.0
24	35.58749280641338	35.0	34.0	40.0	30.0	41.0
25	35.465973604444194	35.0	34.0	40.0	30.0	41.0
26	35.32355896485855	35.0	34.0	40.0	30.0	41.0
27	35.30489406112523	35.0	34.0	40.0	30.0	41.0
28	35.34513972471846	36.0	34.0	40.0	30.0	41.0
29	35.43714329624547	36.0	34.0	40.0	30.0	41.0
30	35.40116951639887	36.0	34.0	40.0	30.0	41.0
31	35.254006900831655	35.0	34.0	40.0	30.0	41.0
32	35.109910903932104	35.0	34.0	40.0	29.0	41.0
33	34.956762054947724	35.0	34.0	40.0	29.0	41.0
34	34.86563841880581	35.0	34.0	40.0	29.0	41.0
35	34.758385278893996	35.0	34.0	40.0	28.0	41.0
36	34.56892646212519	35.0	33.0	40.0	27.0	41.0
37	34.50908960306436	35.0	33.0	40.0	27.0	41.0
38	34.51267407644371	35.0	33.0	40.0	27.0	41.0
39	34.50444603327467	35.0	33.0	39.0	27.0	41.0
40	34.3856806490223	35.0	33.0	39.0	27.0	41.0
41	34.42630635115394	35.0	33.0	39.0	27.0	41.0
42	34.38464034572649	35.0	33.0	39.0	27.0	41.0
43	34.27819029824255	35.0	33.0	39.0	27.0	41.0
44	34.220079899970514	35.0	33.0	39.0	27.0	41.0
45	34.115048523821734	35.0	33.0	39.0	27.0	41.0
46	34.04791367194906	35.0	33.0	39.0	26.0	41.0
47	33.97467690459863	35.0	33.0	39.0	26.0	41.0
48	33.92430759901674	35.0	33.0	38.0	26.0	41.0
49	33.92256276273818	35.0	33.0	38.0	26.0	40.0
50	33.75317165129668	35.0	33.0	38.0	26.0	40.0
51	33.66560784867283	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	19.0
15	36.0
16	136.0
17	421.0
18	975.0
19	2047.0
20	3515.0
21	5954.0
22	9377.0
23	13630.0
24	19556.0
25	26313.0
26	34603.0
27	42764.0
28	51200.0
29	63372.0
30	78113.0
31	98966.0
32	127149.0
33	192189.0
34	293275.0
35	406932.0
36	160669.0
37	199562.0
38	256462.0
39	307064.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.07399976780063	24.66308121882953	31.121397673161844	12.141521340207992
2	27.55550859597059	26.542434434999628	32.2592163521813	13.642840616848487
3	28.62249811442418	26.021990449756814	30.741317372271553	14.614194063547457
4	21.711643462042503	39.28249559197058	25.87348308243829	13.132377863548625
5	23.049295592304677	32.543201194072736	29.71429454406651	14.693208669556073
6	21.886127089898743	40.20185475511737	26.931076043623413	10.980942111360479
7	89.67961498337435	4.388066956943722	4.169398628687313	1.762919430994607
8	92.69854090258735	2.2926764485523874	3.3002379625925142	1.7085446862677458
9	89.7944617944334	3.536989443281127	4.4933919233040465	2.17515683898143
10	64.98337435800633	18.32002920032374	7.304674808122301	9.39192163354763
11	56.62653571067624	16.399539944556135	13.692329151795747	13.281595192971876
12	49.05909139885086	20.07417851120111	17.702905081407597	13.163825008540424
13	21.1510239741674	46.170882031861424	18.579332418456676	14.098761575514493
14	14.475208540927644	45.97255202978165	25.621321247829393	13.930918181461301
15	13.612395771298655	20.80502185430408	52.06026826544564	13.522314108951619
16	16.16295551377452	15.124656816847319	48.56537539872725	20.14701227065092
17	16.270786228739766	16.42902425314075	26.114201998418036	41.18598751970145
18	23.847585335356865	21.971907217140657	32.488784686869124	21.691722760633354
19	32.662558352620636	23.689389073432636	20.9387870673303	22.70926550661643
20	36.10148950049572	22.474268049951263	21.084872210997396	20.33937023855562
21	24.554832879096793	30.22818182045977	24.636144421326595	20.580840879116842
22	25.645251147006427	24.118623809456192	21.806486046738893	28.429638996798488
23	21.502371691054563	31.098595360857033	21.593038028076077	25.805994920012328
24	22.77053106000683	21.56977632852703	38.14196235196246	17.51773025950368
25	18.03525087137408	23.469843733164502	34.80789678320347	23.687008612257955
26	17.584800797162156	36.766431655289175	24.201856425616437	21.44691112193223
27	19.608526895452652	34.39006320333231	25.68116687701034	20.320243024204697
28	14.949839089177086	28.44989379802163	38.649125869599175	17.951141243202112
29	16.483482522821106	24.36827989546017	36.296269692051844	22.851967889666877
30	20.327969082403214	30.50114136848954	28.638576667972437	20.532312881134803
31	33.76609003822937	25.744269979377687	20.36463653698861	20.12500344540433
32	35.45826383361161	25.092566529713583	22.1529057913697	17.296263845305106
33	32.28673782435872	26.48033363207425	21.127094074990374	20.10583446857666
34	20.14530000910422	27.12076121301619	26.745024209707775	25.98891456817181
35	21.254219054213543	25.493611593931746	30.22266917352894	23.029500178325776
36	33.77594598274207	24.16819586935695	22.83363416237418	19.222223985526796
37	23.160258493026085	31.668778455907592	26.41977804078858	18.751185010277744
38	21.335405309013094	34.54825946525654	19.996333254541458	24.120001971188902
39	21.750983715139817	32.50574025242911	24.04244905186649	21.700826980564578
40	25.990000392567282	24.724305009743187	21.091261869939952	28.19443272774958
41	16.885947511248723	23.51657594464634	24.676946361110115	34.92053018299482
42	22.63664055955037	24.149026892529278	21.421018386348013	31.79331416157234
43	22.81801499607015	24.46842631470365	24.30463388089509	28.408924808331115
44	19.673801646610933	30.538978172423903	25.981104985019797	23.806115195945367
45	17.74587866998205	37.536824063873205	19.966264271282366	24.751032994862378
46	22.505422857605826	32.56600350637755	24.254184808982608	20.674388827034022
47	21.614044553880696	25.57838942173169	26.095116546543863	26.71244947784375
48	24.081496967626563	21.118825104594123	30.612814231315678	24.186863696463636
49	21.639018514976442	20.96739436390319	32.44130075080581	24.952286370314564
50	19.000005846746745	33.57139337162674	24.22862617321238	23.199974608414138
51	17.330801413576314	34.639677526859536	22.038977754799134	25.990543304765012
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	483.0
1	786.5
2	1090.0
3	3184.0
4	5278.0
5	3923.0
6	2568.0
7	2828.5
8	3089.0
9	3476.0
10	3863.0
11	4070.0
12	4277.0
13	4290.5
14	4304.0
15	4072.5
16	3841.0
17	3963.0
18	4085.0
19	3779.5
20	3474.0
21	3220.5
22	2967.0
23	3146.5
24	3326.0
25	4452.5
26	7442.5
27	9306.0
28	10327.5
29	11349.0
30	15417.5
31	19486.0
32	23315.0
33	27144.0
34	31059.5
35	34975.0
36	40622.5
37	46270.0
38	66642.0
39	87014.0
40	131500.0
41	175986.0
42	224019.0
43	272052.0
44	265941.5
45	259831.0
46	240489.0
47	221147.0
48	205404.5
49	189662.0
50	184970.5
51	180279.0
52	161019.0
53	141759.0
54	133060.0
55	124361.0
56	123199.5
57	122038.0
58	118258.5
59	114479.0
60	107706.0
61	100933.0
62	88596.5
63	76260.0
64	63148.0
65	50036.0
66	40974.5
67	31913.0
68	26406.5
69	20900.0
70	16871.5
71	12843.0
72	9844.0
73	6845.0
74	5245.5
75	3687.5
76	3729.0
77	2400.5
78	1072.0
79	880.5
80	689.0
81	405.0
82	121.0
83	89.5
84	58.0
85	68.0
86	78.0
87	42.5
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2394494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.206421751568843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86781138065494	11.9929720247742
2	9.154624892072828	2.784181741725397
3	2.9869203363252406	1.362611111174984
4	1.393641451769657	0.8476919874431237
5	0.7286396873537974	0.5540001195416553
6	0.46057529058733543	0.4202221270213438
7	0.3304339615110637	0.351730272584523
8	0.22690771599342302	0.2760363542464955
9	0.1922600705438256	0.2631228946808211
>10	2.2792320029831217	8.7233460599922
>50	1.2802472253315405	14.331117682953654
>100	2.079539720171178	55.86387372494508
>500	0.01736942738620347	1.6949656768815082
>1k	0.0017968373158141517	0.5341282220350136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3386	0.14140774627123726	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05901037964597113	0.0
2	0.0	0.0	0.0	0.2208817395240915	0.0
3	0.0	0.0	0.0	0.32424386947722567	0.0
4	0.0	0.0	0.0	0.5099616035788772	0.0
5	0.0	0.0	0.0	0.8473606532319563	0.0
6	0.0	0.0	0.0	1.1711868979416946	0.0
7	0.0	0.0	0.0	1.337735655215674	0.0
8	0.0	0.0	0.0	1.7606224947734261	0.0
9	0.0	0.0	0.0	1.8842811884264483	0.0
10	0.0	0.0	0.0	2.080481304192034	0.0
11	0.0	0.0	0.0	2.374489140503171	0.0
12	4.176247674874107E-5	0.0	0.0	2.6051015371097193	0.0
13	4.176247674874107E-5	0.0	0.0	2.709841828795562	0.0
14	4.176247674874107E-5	0.0	0.0	2.752523080032775	0.0
15	4.176247674874107E-5	0.0	0.0	2.8184242683422887	0.0
16	4.176247674874107E-5	0.0	0.0	2.9711496458124347	0.0
17	8.352495349748214E-5	0.0	0.0	3.14513212394769	0.0
18	8.352495349748214E-5	0.0	0.0	3.36572152613454	0.0
19	8.352495349748214E-5	0.0	0.0	3.486122746601161	0.0
20	8.352495349748214E-5	0.0	0.0	3.597545034566802	0.0
21	8.352495349748214E-5	0.0	0.0	3.7665995404457058	0.0
22	8.352495349748214E-5	0.0	0.0	3.9194502053460982	0.0
23	8.352495349748214E-5	0.0	0.0	4.105543801738488	0.0
24	1.2528743024622322E-4	0.0	0.0	4.239476064671701	0.0
25	1.2528743024622322E-4	0.0	0.0	4.3495201909046335	0.0
26	1.2528743024622322E-4	0.0	0.0	4.4551374946022	0.0
27	1.2528743024622322E-4	0.0	0.0	4.550272416635832	0.0
28	1.6704990699496427E-4	0.0	0.0	4.665620377415855	0.0
29	1.6704990699496427E-4	0.0	0.0	4.782137687544842	0.0
30	2.0881238374370536E-4	0.0	0.0	4.938705212875873	0.0
31	2.0881238374370536E-4	0.0	0.0	5.085040931403461	0.0
32	2.5057486049244644E-4	0.0	0.0	5.205108052056092	0.0
33	2.923373372411875E-4	0.0	0.0	5.332400081186255	0.0
34	2.923373372411875E-4	0.0	0.0	5.4774829254113815	0.0
35	2.923373372411875E-4	0.0	0.0	5.665372308303968	0.0
36	2.923373372411875E-4	0.0	0.0	5.818306498157857	0.0
37	2.923373372411875E-4	0.0	0.0	5.968024977302094	0.0
38	2.923373372411875E-4	0.0	0.0	6.1345319720993245	0.0
39	2.923373372411875E-4	0.0	0.0	6.2986167432451285	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGC	40	6.8212103E-9	45.0	23
CGTTGTA	20	7.0348196E-4	45.0	19
TATGCGA	20	7.0348196E-4	45.0	40
TACGCGA	40	6.8212103E-9	45.0	37
ACCGTAG	20	7.0348196E-4	45.0	1
TAGTGAT	40	6.8212103E-9	45.0	24
AGCTAAC	40	6.8212103E-9	45.0	23
ACTCACG	20	7.0348196E-4	45.0	1
TAAGCTT	40	6.8212103E-9	45.0	1
CGAAGCA	20	7.0348196E-4	45.0	43
CGGAATA	45	3.8562575E-10	44.999996	23
AACGTAG	55	1.8189894E-12	44.999996	1
TCCGCTA	30	2.166491E-6	44.999996	14
CTCCGTA	25	3.8920778E-5	44.999996	37
GGTCGAC	25	3.8920778E-5	44.999996	13
CACATCG	45	3.8562575E-10	44.999996	19
GCACGAC	25	3.8920778E-5	44.999996	35
ACTTACG	60	0.0	44.999996	1
ATATCGT	55	1.8189894E-12	44.999996	29
CCGTAGA	30	2.166491E-6	44.999996	19
>>END_MODULE