FastQCFastQC Report
Thu 26 May 2016
SRR1545324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545324_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3121631
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG63080.20207385177812495No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG41380.13255890910873194No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.1257035184491697No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG37550.12028968190026304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA502.1827873E-1145.026
CTATCGT207.03515E-445.013
ACGCACT207.03515E-445.011
CACGCGA502.1827873E-1145.013
ACCGAAT207.03515E-445.013
CCCCGTT351.2128294E-745.042
TACGATA900.045.013
TATTACG453.8562575E-1045.01
AACGACA207.03515E-445.037
GTATCGC502.1827873E-1145.022
TACTCGA351.2128294E-745.045
TCATACG406.8212103E-945.01
ATACGAG1050.045.01
GCCGTAT253.89235E-545.09
TCGAACA253.89235E-545.01
AATCGTC453.8562575E-1045.042
ATCGACT453.8562575E-1045.028
TACGTCG253.89235E-545.044
TACGTCA406.8212103E-945.041
TAGTACT253.89235E-545.024