##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545324_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3121631
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.320289297485832	31.0	31.0	33.0	30.0	34.0
2	31.640724031764165	31.0	31.0	34.0	30.0	34.0
3	31.732461652258067	31.0	31.0	34.0	30.0	34.0
4	30.306114656088436	35.0	33.0	37.0	0.0	37.0
5	32.886504843141296	35.0	33.0	37.0	19.0	37.0
6	34.528680680067566	35.0	33.0	37.0	30.0	37.0
7	35.52012585728422	36.0	35.0	37.0	33.0	37.0
8	35.70005871930411	37.0	35.0	37.0	35.0	37.0
9	37.733576133758284	39.0	37.0	39.0	35.0	39.0
10	37.20646450525383	39.0	37.0	39.0	34.0	39.0
11	36.76043516994802	39.0	35.0	39.0	32.0	39.0
12	35.55490671383004	37.0	35.0	39.0	31.0	39.0
13	35.07132777704988	37.0	35.0	39.0	30.0	39.0
14	35.88869055951841	37.0	35.0	40.0	30.0	41.0
15	36.22218032816819	37.0	35.0	40.0	31.0	41.0
16	36.40453339936719	37.0	35.0	40.0	32.0	41.0
17	36.348158382589105	37.0	35.0	40.0	31.0	41.0
18	36.324066809946466	37.0	35.0	40.0	31.0	41.0
19	36.31436867458069	37.0	35.0	40.0	31.0	41.0
20	36.19834054697689	37.0	35.0	40.0	31.0	41.0
21	36.02938047450195	36.0	34.0	40.0	31.0	41.0
22	35.8980398387894	36.0	34.0	40.0	30.0	41.0
23	35.883671708795816	36.0	34.0	40.0	30.0	41.0
24	35.7885576482294	36.0	34.0	40.0	30.0	41.0
25	35.65894623675892	36.0	34.0	40.0	30.0	41.0
26	35.52919099022274	35.0	34.0	40.0	30.0	41.0
27	35.52857016091908	36.0	34.0	40.0	30.0	41.0
28	35.549881135854946	36.0	34.0	40.0	30.0	41.0
29	35.60440679888174	36.0	34.0	40.0	30.0	41.0
30	35.576249402956336	36.0	34.0	40.0	30.0	41.0
31	35.425673309881915	36.0	34.0	40.0	30.0	41.0
32	35.31421234604603	35.0	34.0	40.0	29.0	41.0
33	35.18525572048714	35.0	34.0	40.0	29.0	41.0
34	35.09665299966588	35.0	34.0	40.0	29.0	41.0
35	34.978204022192244	35.0	34.0	40.0	28.0	41.0
36	34.810504829046096	35.0	33.0	40.0	27.0	41.0
37	34.77485807899781	35.0	33.0	40.0	27.0	41.0
38	34.7586697466805	35.0	33.0	40.0	27.0	41.0
39	34.70688976371647	35.0	33.0	40.0	27.0	41.0
40	34.60063056780254	35.0	33.0	40.0	27.0	41.0
41	34.57748625638328	35.0	34.0	40.0	27.0	41.0
42	34.50565842022968	35.0	33.0	39.0	27.0	41.0
43	34.42210754570287	35.0	33.0	39.0	27.0	41.0
44	34.349942385887374	35.0	33.0	39.0	27.0	41.0
45	34.25998972972783	35.0	33.0	39.0	27.0	41.0
46	34.200087710558996	35.0	33.0	39.0	26.0	41.0
47	34.12251896524605	35.0	33.0	39.0	26.0	41.0
48	34.056824140969894	35.0	33.0	39.0	26.0	41.0
49	34.02709288830102	35.0	33.0	39.0	26.0	41.0
50	33.883151147589196	35.0	33.0	38.0	26.0	40.0
51	33.7842333062428	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	4.0
14	17.0
15	68.0
16	217.0
17	627.0
18	1365.0
19	2661.0
20	4494.0
21	7469.0
22	11563.0
23	16949.0
24	24169.0
25	33116.0
26	43591.0
27	54332.0
28	64927.0
29	79089.0
30	97903.0
31	123648.0
32	160732.0
33	236750.0
34	362827.0
35	493862.0
36	226012.0
37	280866.0
38	356730.0
39	437255.0
40	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.797416158412062	25.62964680963253	30.5632536324761	12.00968339947931
2	28.179147375202255	27.038045175743065	30.948052476413775	13.834754972640905
3	29.385407820463083	25.745707932808205	30.733997708249312	14.134886538479405
4	23.104524525800777	38.49266617354838	25.607991463436903	12.794817837213943
5	24.964898157405536	32.107190119524056	28.705378694663143	14.222533028407266
6	21.868311789574104	42.23032767165626	24.89192348487057	11.009437053899067
7	89.8224037370208	4.306210439350455	4.049966187547471	1.8214196360812667
8	91.94526835490807	2.7262991686076923	3.4585766222849528	1.8698558541992951
9	88.44575159588048	4.624761863269554	4.462346766802354	2.467139774047605
10	61.381374031716106	20.619861860674757	8.588875494893534	9.409888612715596
11	54.01708273655663	19.242569028818586	13.854264004938443	12.88608422968634
12	48.61282451385189	20.764433720705618	17.41634421236847	13.206397553074018
13	22.514896860006836	42.62845288248355	18.924177777578453	15.932472479931164
14	15.1035147972326	43.767729113402574	24.134979438633202	16.993776650731622
15	15.654060329359876	22.530145299043994	46.69168136784905	15.12411300374708
16	17.8662051985004	17.82744341019166	45.03210020659072	19.27425118471722
17	18.24568630949654	20.45446755237887	24.295440428417066	37.00440570970752
18	23.773309529537602	24.141578553006426	30.753186395188926	21.331925522267046
19	34.50907554416265	25.21364632783311	19.324513371375414	20.952764756628827
20	36.58846929697969	24.31824901790122	20.562776317892794	18.5305053672263
21	25.995545277452713	29.78359710036196	23.12778159878602	21.093076023399306
22	25.423472537272986	25.549464366544285	21.901211257832845	27.12585183834989
23	23.993450859502612	30.71221422391051	21.89240176048995	23.401933156096923
24	23.74499099989717	22.875221318599156	35.47437221119345	17.90541547031023
25	19.199354440034714	25.50612163961724	33.1334164736319	22.161107446716155
26	18.8636965740025	37.638881725610744	22.530273437187162	20.96714826319959
27	20.99130230318702	33.849932935699314	26.843563508947728	18.315201252165934
28	16.843566712401305	28.617475928448943	37.018949388957246	17.520007970192506
29	17.293203456782688	26.04641612029096	35.02025063180113	21.640129791125215
30	21.15346112336788	31.383497921439147	27.739825751346007	19.723215203846962
31	33.96115684397035	25.331629523156323	20.198063127896923	20.509150504976407
32	34.88749951547764	26.455465107823446	21.163423863999302	17.493611512699612
33	30.856882187548752	26.929095719513292	21.588554188499536	20.625467904438416
34	19.82418806066444	27.44292967362254	26.252334116364167	26.48054814934885
35	22.63995968774016	24.028048158158345	29.21882823434288	24.113163919758613
36	31.95329620957762	25.91517062714972	23.224878276772625	18.90665488650004
37	22.44442088126367	33.4950543481917	25.52047311165221	18.540051658892416
38	22.735294466258182	33.78333313578703	20.325944994779974	23.155427403174812
39	22.957902455479203	31.56362811619951	24.519201660926612	20.959267767394675
40	27.120726312623113	24.997733556592692	22.432536068484712	25.449004062299487
41	17.884849298331545	23.161738206725907	25.846616720554096	33.10679577438845
42	22.222069168328993	25.321570678917528	22.19778699019839	30.258573162555084
43	23.370507276484634	25.13378423010279	23.921501292113	27.57420720129958
44	20.47410472281958	29.759122715016606	26.602952110611405	23.16382045155241
45	19.02790560447407	36.534523138705374	20.093021885033817	24.344549371786737
46	22.50579905184181	33.03574317400103	22.477352384058204	21.981105390098957
47	22.395568214180344	25.44298156957052	26.995887726640337	25.165562489608796
48	24.382189951342745	21.38116260377988	30.534070170369272	23.7025772745081
49	21.740013473725757	23.580173313245545	30.847784379383725	23.832028833644976
50	19.941338358057052	33.29519087938325	24.018630004635398	22.744840757924305
51	18.332531936029596	34.438054978311015	22.44615074619646	24.783262339462926
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	503.0
1	1193.0
2	1883.0
3	5091.5
4	8300.0
5	6140.0
6	3980.0
7	4225.5
8	4471.0
9	4785.0
10	5099.0
11	5188.5
12	5278.0
13	5267.5
14	5257.0
15	4943.0
16	4629.0
17	4423.5
18	4218.0
19	4573.0
20	4928.0
21	4937.5
22	4947.0
23	5472.5
24	5998.0
25	8139.5
26	13134.0
27	15987.0
28	26309.0
29	36631.0
30	41201.0
31	45771.0
32	47743.0
33	49715.0
34	54460.0
35	59205.0
36	63500.0
37	67795.0
38	87897.5
39	108000.0
40	165622.0
41	223244.0
42	269909.0
43	316574.0
44	320719.0
45	324864.0
46	292209.5
47	259555.0
48	241974.5
49	224394.0
50	215162.5
51	205931.0
52	193625.0
53	181319.0
54	169338.0
55	157357.0
56	154853.5
57	152350.0
58	149790.0
59	147230.0
60	143514.5
61	139799.0
62	125603.0
63	111407.0
64	92926.5
65	74446.0
66	62275.0
67	50104.0
68	42388.5
69	34673.0
70	30034.0
71	25395.0
72	21971.0
73	18547.0
74	14773.5
75	8670.5
76	6341.0
77	4257.0
78	2173.0
79	1652.0
80	1131.0
81	902.5
82	674.0
83	382.0
84	90.0
85	76.0
86	62.0
87	55.0
88	48.0
89	37.0
90	26.0
91	19.0
92	12.0
93	10.0
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3121631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.501649210932577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.11105746746898	11.327391548882309
2	9.602542772417442	2.7850541363714743
3	3.3731088398306275	1.467469234365586
4	1.6273088283359347	0.943946471455257
5	0.9026822262924573	0.6545190497318438
6	0.5711783712354446	0.4969817025916944
7	0.3850279538431211	0.3908478226125257
8	0.27506741013008723	0.3191144872852998
9	0.20147823695647774	0.26295900443819925
>10	1.9297621653241313	6.548844973705467
>50	0.9285929687162298	9.934411918601386
>100	2.0547890826944872	60.336634820961834
>500	0.03161213970869987	2.915603103085868
>1k	0.005550223002290816	1.3954763183752839
>5k	2.413140435778616E-4	0.22074540753595534
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6308	0.20207385177812495	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4138	0.13255890910873194	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3924	0.1257035184491697	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3755	0.12028968190026304	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06983528802731649	0.0
2	0.0	0.0	0.0	0.20726344657648518	0.0
3	0.0	0.0	0.0	0.2954545236128165	0.0
4	0.0	0.0	0.0	0.4892954996923083	0.0
5	0.0	0.0	0.0	0.8013759473813529	0.0
6	0.0	0.0	0.0	1.1130719806408893	0.0
7	0.0	0.0	0.0	1.2748143518564494	0.0
8	0.0	0.0	0.0	1.6546798772821003	0.0
9	0.0	0.0	0.0	1.75731852996078	0.0
10	0.0	0.0	0.0	1.9335725458902733	0.0
11	0.0	0.0	0.0	2.2527966950610114	0.0
12	0.0	0.0	0.0	2.4940167495773844	0.0
13	0.0	0.0	0.0	2.5979047491519656	0.0
14	0.0	0.0	0.0	2.63781978074923	0.0
15	0.0	0.0	0.0	2.7060853765227217	0.0
16	0.0	0.0	0.0	2.8546935880634194	0.0
17	0.0	0.0	0.0	3.0157632340273404	0.0
18	3.203453579234701E-5	0.0	0.0	3.20370985552104	0.0
19	3.203453579234701E-5	0.0	0.0	3.3164393869743094	0.0
20	6.406907158469402E-5	0.0	0.0	3.4335576498311298	0.0
21	6.406907158469402E-5	0.0	0.0	3.602764067886307	0.0
22	6.406907158469402E-5	0.0	0.0	3.767261409180009	0.0
23	6.406907158469402E-5	0.0	0.0	3.9431950797515785	0.0
24	6.406907158469402E-5	0.0	0.0	4.082385137769326	0.0
25	6.406907158469402E-5	0.0	0.0	4.202770923276966	0.0
26	6.406907158469402E-5	0.0	0.0	4.315756731016575	0.0
27	6.406907158469402E-5	0.0	0.0	4.411283716749353	0.0
28	6.406907158469402E-5	0.0	0.0	4.523340522950983	0.0
29	6.406907158469402E-5	0.0	0.0	4.646801623894689	0.0
30	6.406907158469402E-5	0.0	0.0	4.804155263706697	0.0
31	6.406907158469402E-5	0.0	0.0	4.955966928826629	0.0
32	9.610360737704103E-5	0.0	0.0	5.094420192521153	0.0
33	9.610360737704103E-5	0.0	0.0	5.228805070170049	0.0
34	9.610360737704103E-5	0.0	0.0	5.37664445285173	0.0
35	9.610360737704103E-5	0.0	0.0	5.570101014501714	0.0
36	9.610360737704103E-5	0.0	0.0	5.72537240948722	0.0
37	1.2813814316938805E-4	0.0	0.0	5.886666297201687	0.0
38	1.2813814316938805E-4	0.0	0.0	6.040656310755499	0.0
39	1.2813814316938805E-4	0.0	0.0	6.220850574587451	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	50	2.1827873E-11	45.0	26
CTATCGT	20	7.03515E-4	45.0	13
ACGCACT	20	7.03515E-4	45.0	11
CACGCGA	50	2.1827873E-11	45.0	13
ACCGAAT	20	7.03515E-4	45.0	13
CCCCGTT	35	1.2128294E-7	45.0	42
TACGATA	90	0.0	45.0	13
TATTACG	45	3.8562575E-10	45.0	1
AACGACA	20	7.03515E-4	45.0	37
GTATCGC	50	2.1827873E-11	45.0	22
TACTCGA	35	1.2128294E-7	45.0	45
TCATACG	40	6.8212103E-9	45.0	1
ATACGAG	105	0.0	45.0	1
GCCGTAT	25	3.89235E-5	45.0	9
TCGAACA	25	3.89235E-5	45.0	1
AATCGTC	45	3.8562575E-10	45.0	42
ATCGACT	45	3.8562575E-10	45.0	28
TACGTCG	25	3.89235E-5	45.0	44
TACGTCA	40	6.8212103E-9	45.0	41
TAGTACT	25	3.89235E-5	45.0	24
>>END_MODULE