FastQCFastQC Report
Thu 26 May 2016
SRR1545323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545323_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3193244
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT54110.16945150448885207No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.1177485967248353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT28300.041.6607781
TATAGCG2000.040.51
CCCGATA451.9301297E-840.0000049
ATAGGGC17100.039.6052634
GCTACGA7000.038.8928610
CGTATTT6750.038.6666681
TACGAAT7050.038.61702312
ATACGCA700.038.5714336
ATAGGGA41950.038.295594
TATGGGC8350.038.263474
GGCGATA11450.037.9257628
TTAATCG11050.037.87330220
CGAATAT7150.037.76223814
CGTTGAT10950.037.60273725
ATAGGGT10300.037.5728154
TCGATAG900.037.5000041
TACGAAC301.1403767E-437.49999617
TACCTAC301.1403767E-437.49999628
ACCGTTA301.1403767E-437.49999642
GGCCTAT11950.037.4686168
Produced by FastQC (version 0.11.5)