##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545323_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3193244
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201404903602732	31.0	31.0	33.0	30.0	34.0
2	31.52626670558216	31.0	31.0	34.0	30.0	34.0
3	31.642589479538675	31.0	31.0	34.0	30.0	34.0
4	30.330108190918075	35.0	33.0	37.0	0.0	37.0
5	32.85594680519246	35.0	33.0	37.0	19.0	37.0
6	34.4650076849749	35.0	33.0	37.0	30.0	37.0
7	35.44263012785744	36.0	35.0	37.0	33.0	37.0
8	35.616936882994224	37.0	35.0	37.0	35.0	37.0
9	37.63935389841804	39.0	37.0	39.0	35.0	39.0
10	37.06277096269499	39.0	37.0	39.0	33.0	39.0
11	36.61271891530995	39.0	35.0	39.0	32.0	39.0
12	35.707173645358765	37.0	35.0	39.0	31.0	39.0
13	35.31653140192231	37.0	35.0	39.0	30.0	39.0
14	36.09736556304498	38.0	35.0	40.0	30.0	41.0
15	36.4066945714139	38.0	35.0	40.0	31.0	41.0
16	36.57117840039784	38.0	35.0	40.0	31.0	41.0
17	36.49475705583413	38.0	35.0	40.0	31.0	41.0
18	36.470955241754154	38.0	35.0	40.0	31.0	41.0
19	36.45649095402669	38.0	35.0	40.0	31.0	41.0
20	36.348078004687395	38.0	35.0	40.0	31.0	41.0
21	36.2054797566362	38.0	34.0	40.0	30.0	41.0
22	36.11278248702573	38.0	34.0	40.0	30.0	41.0
23	36.053165370388236	37.0	34.0	40.0	30.0	41.0
24	35.98576024882533	37.0	34.0	40.0	30.0	41.0
25	35.82200890379814	37.0	34.0	40.0	30.0	41.0
26	35.67687498982226	37.0	34.0	40.0	30.0	41.0
27	35.66677867397543	37.0	34.0	40.0	30.0	41.0
28	35.69093467332907	37.0	34.0	40.0	30.0	41.0
29	35.66738025656667	37.0	34.0	40.0	30.0	41.0
30	35.606497968836706	37.0	34.0	40.0	29.0	41.0
31	35.49091268941553	37.0	34.0	40.0	29.0	41.0
32	35.413968678873275	37.0	34.0	40.0	29.0	41.0
33	35.276389151596305	36.0	34.0	40.0	29.0	41.0
34	35.13751532923886	36.0	34.0	40.0	27.0	41.0
35	35.04039058712707	36.0	34.0	40.0	27.0	41.0
36	34.87445369035376	36.0	33.0	40.0	26.0	41.0
37	34.8139446907283	36.0	33.0	40.0	27.0	41.0
38	34.77001820092671	36.0	33.0	40.0	27.0	41.0
39	34.66725405261859	36.0	33.0	40.0	26.0	41.0
40	34.531266010364384	35.0	33.0	40.0	26.0	41.0
41	34.46228568815913	35.0	33.0	40.0	25.0	41.0
42	34.402095173434915	35.0	33.0	40.0	26.0	41.0
43	34.34048885709955	35.0	33.0	40.0	26.0	41.0
44	34.25239505656317	35.0	33.0	40.0	26.0	41.0
45	34.158508087700156	35.0	33.0	39.0	25.0	41.0
46	34.08978581029198	35.0	33.0	39.0	25.0	41.0
47	34.01598374568307	35.0	33.0	39.0	25.0	41.0
48	33.90129661247308	35.0	33.0	39.0	24.0	41.0
49	33.83518077541209	35.0	33.0	39.0	24.0	41.0
50	33.711152044754485	35.0	32.0	39.0	24.0	40.0
51	33.6079817264199	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	4.0
13	13.0
14	22.0
15	109.0
16	298.0
17	872.0
18	1820.0
19	3465.0
20	5919.0
21	9258.0
22	13841.0
23	19912.0
24	27391.0
25	36329.0
26	47492.0
27	57997.0
28	70011.0
29	84258.0
30	102971.0
31	127589.0
32	160691.0
33	227950.0
34	329808.0
35	418277.0
36	261215.0
37	326812.0
38	401471.0
39	457188.0
40	254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.04044413768569	24.712079628114857	29.935764382552666	12.31171185164679
2	28.308704251851722	26.002835987478562	31.25708527127899	14.431374489390727
3	29.3487124692006	25.68475819574076	31.373737803938567	13.592791531120078
4	23.274826477400413	38.746647609766114	25.33317215972221	12.645353753111255
5	23.54937486768941	32.288763401731906	29.145877984895613	15.015983745683073
6	22.704340789491813	41.32775321898358	24.993924673466857	10.97398131805775
7	89.92811072376556	3.922969870138329	4.204063328702723	1.944856077393397
8	91.78750512018499	2.7107856461955304	3.616541673608406	1.8851675600110733
9	88.306969339017	4.415666325529775	4.796846091310279	2.4805182441429467
10	57.30269907341875	22.396409419386682	10.044957416345259	10.255934090849305
11	46.60110533363564	21.310397827413126	17.629658115696763	14.458838723254472
12	43.149536959906605	20.64145427032823	20.57218302140394	15.636825748361227
13	24.677913745394964	34.66575056588222	21.44067287059805	19.215662818124766
14	17.72492174102574	35.23701915669457	26.52547064990962	20.512588452370068
15	17.406186310848778	23.265462958671495	40.79193447165328	18.536416258826446
16	21.586887816903438	18.904317991359257	38.50945934604433	20.999334845692967
17	21.6946778886925	21.169381356388676	25.172269954942372	31.96367079997645
18	25.319549649196865	25.0354811595982	27.825872373047595	21.819096818157334
19	32.845062889024454	25.78346659384626	20.052210228845652	21.31926028828364
20	34.15038124239801	24.37781140432739	22.211018011777366	19.260789341497237
21	26.878090117761122	27.233026978207743	24.532606966457934	21.3562759375732
22	26.56759082613167	24.04457661237287	23.43679343012936	25.951039131366098
23	25.63296133962829	28.32849603725866	23.10932080354649	22.929221819566557
24	24.757018254790424	22.924493086027876	32.52592034933754	19.792568309844157
25	22.94998440457416	25.30746162836288	28.877029127745953	22.865524839317008
26	21.368332642291037	32.271069796107035	23.868642671840924	22.491954889761008
27	21.509161216618587	30.774409973055615	26.802712226187538	20.91371658413826
28	20.137484013122705	27.140206009938485	32.38430887210623	20.33800110483258
29	20.7004225170391	24.57861034108261	31.652012812049442	23.06895432982885
30	24.112877061696505	28.196686504382377	26.11219186507514	21.578244568845975
31	30.596816278367704	25.162749855632704	22.418581229621036	21.821852636378555
32	33.225177906855855	24.98424799357644	21.74096310836253	20.04961099120518
33	30.654719777129465	25.83739294585694	21.88338880461374	21.624498472399853
34	22.325697629119478	25.234902187242813	26.550053801087547	25.88934638255016
35	23.984136508202944	24.55737801433276	27.600020543372196	23.8584649340921
36	30.488713045417136	25.421170446104334	22.765689061030102	21.324427447448425
37	23.675422235194056	30.41418068897961	25.447632564251276	20.462764511575063
38	24.636952265470473	29.240922397411534	23.081793937450442	23.04033139966755
39	24.16980976085761	29.222665101695956	23.79661560469541	22.81090953275102
40	27.49774210802557	23.99528504555242	24.110497036869088	24.39647580955292
41	20.08737822728235	22.56416985360342	26.512317881126528	30.8361340379877
42	22.56824094870295	24.903765575070366	23.928487769804	28.599505706422683
43	23.333231034020578	25.48652718050985	25.027589498328346	26.152652287141226
44	22.952145216588523	27.41206121423856	26.056668391140796	23.579125178032122
45	21.07549564017031	30.676735006783073	21.858774337319666	26.38899501572695
46	22.41914491971174	30.013177821676013	24.401455072020802	23.166222186591444
47	22.662659038895868	24.677976377627264	27.18386067585189	25.475503907624976
48	23.993030285189608	22.159659581291	30.04123706174661	23.80607307177278
49	22.315175414093	24.145445822492736	29.34843062415525	24.19094813925901
50	20.695975628545767	29.556745428786524	26.136493171207714	23.610785771459994
51	19.96909725658296	30.499391841024366	23.960712053322577	25.570798849070098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	487.0
1	1148.5
2	1810.0
3	5014.0
4	8218.0
5	6125.0
6	4032.0
7	4278.5
8	4525.0
9	4868.0
10	5211.0
11	5301.5
12	5392.0
13	5360.0
14	5328.0
15	5166.5
16	5005.0
17	4924.5
18	4844.0
19	4992.0
20	5140.0
21	5733.0
22	6326.0
23	6939.5
24	7553.0
25	8712.0
26	15562.5
27	21254.0
28	25219.0
29	29184.0
30	32899.0
31	36614.0
32	46486.0
33	56358.0
34	66983.0
35	77608.0
36	76886.5
37	76165.0
38	92837.5
39	109510.0
40	151594.0
41	193678.0
42	221139.5
43	248601.0
44	252573.0
45	256545.0
46	244037.5
47	231530.0
48	228387.0
49	225244.0
50	215347.0
51	205450.0
52	195545.5
53	185641.0
54	179075.0
55	172509.0
56	174032.0
57	175555.0
58	179313.5
59	183072.0
60	175831.5
61	168591.0
62	151693.0
63	134795.0
64	119779.0
65	104763.0
66	90766.0
67	76769.0
68	64453.0
69	52137.0
70	45391.0
71	38645.0
72	31628.5
73	24612.0
74	20483.5
75	12915.5
76	9476.0
77	7100.5
78	4725.0
79	3386.5
80	2048.0
81	1749.5
82	1451.0
83	949.0
84	447.0
85	276.5
86	106.0
87	75.0
88	44.0
89	30.5
90	17.0
91	10.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3193244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.862431455525144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40927180507354	13.221709712906929
2	8.410235416921513	2.8363403648533794
3	2.9194476488287506	1.4768695759910653
4	1.5004776686194377	1.0120680735056575
5	0.9125140564061388	0.7693602864175861
6	0.6229034279678346	0.6302199814511553
7	0.45637942963151273	0.5386966794911131
8	0.3268172429028025	0.44087466855457746
9	0.26025967992744786	0.3949749912072153
>10	2.976595668270681	12.225907601933121
>50	1.435285176811056	17.769480848760402
>100	1.7615057027947993	47.361233973510544
>500	0.0066861829967907165	0.7641056636426337
>1k	0.0014182812417434854	0.37328944694654215
>5k	2.0261160596335504E-4	0.1848681308280926
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5411	0.16945150448885207	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3760	0.1177485967248353	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04061700264683814	0.0
2	0.0	0.0	0.0	0.12880318572586372	0.0
3	0.0	0.0	0.0	0.18908670931504137	0.0
4	0.0	0.0	0.0	0.3332974241868144	0.0
5	0.0	0.0	0.0	0.5927201303752547	0.0
6	3.1316116150222154E-5	0.0	0.0	0.8679887913357075	0.0
7	6.263223230044431E-5	0.0	0.0	1.0216569732848477	0.0
8	6.263223230044431E-5	0.0	0.0	1.4064694085387774	0.0
9	6.263223230044431E-5	0.0	0.0	1.5254393337934715	0.0
10	6.263223230044431E-5	0.0	0.0	1.7235450845597768	0.0
11	6.263223230044431E-5	0.0	0.0	2.027593256262284	0.0
12	6.263223230044431E-5	0.0	0.0	2.2595204124708292	0.0
13	9.394834845066648E-5	0.0	0.0	2.361297789959051	0.0
14	9.394834845066648E-5	0.0	0.0	2.399315554965421	0.0
15	9.394834845066648E-5	0.0	0.0	2.4606951426198562	0.0
16	9.394834845066648E-5	0.0	0.0	2.58815173535126	0.0
17	9.394834845066648E-5	0.0	0.0	2.734147468843596	0.0
18	9.394834845066648E-5	0.0	0.0	2.89824391747076	0.0
19	9.394834845066648E-5	0.0	0.0	3.0118587868637663	0.0
20	1.2526446460088862E-4	0.0	0.0	3.1329582080166753	0.0
21	1.2526446460088862E-4	0.0	0.0	3.297962824012196	0.0
22	1.2526446460088862E-4	0.0	0.0	3.475305989770904	0.0
23	1.5658058075111077E-4	0.0	0.0	3.6556868187961835	0.0
24	1.5658058075111077E-4	0.0	0.0	3.7977993538858916	0.0
25	1.5658058075111077E-4	0.0	0.0	3.921936438305372	0.0
26	1.5658058075111077E-4	0.0	0.0	4.0350502498399745	0.0
27	1.5658058075111077E-4	0.0	0.0	4.1581225863103475	0.0
28	1.5658058075111077E-4	0.0	0.0	4.281226238896871	0.0
29	1.5658058075111077E-4	0.0	0.0	4.423902464077283	0.0
30	1.8789669690133295E-4	0.0	0.0	4.598646392195523	0.0
31	1.8789669690133295E-4	0.0	0.0	4.755320921295084	0.0
32	1.8789669690133295E-4	0.0	0.0	4.899187158889204	0.0
33	1.8789669690133295E-4	0.0	0.0	5.046717382072901	0.0
34	1.8789669690133295E-4	0.0	0.0	5.209060128195653	0.0
35	1.8789669690133295E-4	0.0	0.0	5.406727453335855	0.0
36	2.192128130515551E-4	0.0	0.0	5.569038883342457	0.0
37	2.5052892920177723E-4	0.0	0.0	5.742530166814688	0.0
38	2.818450453519994E-4	0.0	0.0	5.9639664241129084	0.0
39	2.818450453519994E-4	0.0	0.0	6.373863068403166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	2830	0.0	41.660778	1
TATAGCG	200	0.0	40.5	1
CCCGATA	45	1.9301297E-8	40.000004	9
ATAGGGC	1710	0.0	39.605263	4
GCTACGA	700	0.0	38.89286	10
CGTATTT	675	0.0	38.666668	1
TACGAAT	705	0.0	38.617023	12
ATACGCA	70	0.0	38.57143	36
ATAGGGA	4195	0.0	38.29559	4
TATGGGC	835	0.0	38.26347	4
GGCGATA	1145	0.0	37.925762	8
TTAATCG	1105	0.0	37.873302	20
CGAATAT	715	0.0	37.762238	14
CGTTGAT	1095	0.0	37.602737	25
ATAGGGT	1030	0.0	37.572815	4
TCGATAG	90	0.0	37.500004	1
TACGAAC	30	1.1403767E-4	37.499996	17
TACCTAC	30	1.1403767E-4	37.499996	28
ACCGTTA	30	1.1403767E-4	37.499996	42
GGCCTAT	1195	0.0	37.468616	8
>>END_MODULE