FastQCFastQC Report
Thu 26 May 2016
SRR1545322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545322_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4385622
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52630.1200057825321015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA650.045.00000425
TACGAGT351.2129749E-745.044
CGTAAGT253.8926097E-545.022
ACGTATG2800.044.1964261
GCGATAT5200.044.1346179
TAGTACG1300.043.2692341
TCGACAG1250.043.21
TTACGAG850.042.352941
CGGTCTA2250.042.031
ACTTACG603.6379788E-1241.2499961
ATCGATC556.184564E-1140.90909211
CGCTAGC501.0822987E-940.513
AACGCGC501.0822987E-940.541
TCGTAAG501.0822987E-940.51
CGATATA1400.040.1785710
TTCGTTA451.9304935E-840.00000442
GTTAGCG3100.039.9193531
TATAGCG2200.039.8863681
ATAGCGG9650.039.6373062
CGGGTAT4550.039.560446
Produced by FastQC (version 0.11.5)