##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545322_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4385622
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242347379687534	31.0	31.0	33.0	30.0	34.0
2	31.564927392283238	31.0	31.0	34.0	30.0	34.0
3	31.658864580668375	31.0	31.0	34.0	30.0	34.0
4	30.274889399952848	35.0	33.0	37.0	0.0	37.0
5	32.83786290747356	35.0	33.0	37.0	19.0	37.0
6	34.452781612277576	35.0	33.0	37.0	30.0	37.0
7	35.47647061237836	36.0	35.0	37.0	33.0	37.0
8	35.65554600920918	37.0	35.0	37.0	35.0	37.0
9	37.66742528197825	39.0	37.0	39.0	35.0	39.0
10	37.09913257458121	39.0	37.0	39.0	33.0	39.0
11	36.636691443083784	39.0	35.0	39.0	32.0	39.0
12	35.59299228250862	37.0	35.0	39.0	31.0	39.0
13	35.16931167346388	37.0	35.0	39.0	30.0	39.0
14	36.017087883999125	38.0	35.0	40.0	30.0	41.0
15	36.320765902761345	38.0	35.0	40.0	31.0	41.0
16	36.50941827635852	38.0	35.0	40.0	32.0	41.0
17	36.453936750590906	38.0	35.0	40.0	31.0	41.0
18	36.43005233921209	37.0	35.0	40.0	31.0	41.0
19	36.42039259197441	37.0	35.0	40.0	31.0	41.0
20	36.308465253047345	37.0	35.0	40.0	31.0	41.0
21	36.15704613849529	37.0	34.0	40.0	31.0	41.0
22	36.01316141701223	37.0	34.0	40.0	30.0	41.0
23	35.95814162734499	37.0	34.0	40.0	30.0	41.0
24	35.87602055079074	37.0	34.0	40.0	30.0	41.0
25	35.7719422695344	36.0	34.0	40.0	30.0	41.0
26	35.617338658005636	36.0	34.0	40.0	30.0	41.0
27	35.61177525103623	36.0	34.0	40.0	30.0	41.0
28	35.632428877819386	36.0	34.0	40.0	30.0	41.0
29	35.6742197571975	36.0	34.0	40.0	30.0	41.0
30	35.63055730749253	36.0	34.0	40.0	30.0	41.0
31	35.534931191060245	36.0	34.0	40.0	30.0	41.0
32	35.43390675256554	36.0	34.0	40.0	29.0	41.0
33	35.30223238573684	36.0	34.0	40.0	29.0	41.0
34	35.19855997621318	36.0	34.0	40.0	29.0	41.0
35	35.08168214223661	36.0	34.0	40.0	28.0	41.0
36	34.92697911493512	36.0	33.0	40.0	27.0	41.0
37	34.87128553258808	36.0	33.0	40.0	27.0	41.0
38	34.85297205276697	35.0	33.0	40.0	27.0	41.0
39	34.797513328782095	35.0	33.0	40.0	27.0	41.0
40	34.694899149995145	35.0	33.0	40.0	27.0	41.0
41	34.652917191677716	35.0	33.0	40.0	27.0	41.0
42	34.591801573414216	35.0	33.0	40.0	27.0	41.0
43	34.51138971849375	35.0	33.0	40.0	27.0	41.0
44	34.449804611523746	35.0	33.0	40.0	27.0	41.0
45	34.33660812536967	35.0	33.0	40.0	26.0	41.0
46	34.27021435043878	35.0	33.0	39.0	26.0	41.0
47	34.20057109345037	35.0	33.0	39.0	26.0	41.0
48	34.10088261140609	35.0	33.0	39.0	26.0	41.0
49	34.051001659513744	35.0	33.0	39.0	26.0	41.0
50	33.91617038586545	35.0	33.0	39.0	25.0	41.0
51	33.81503832295624	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	39.0
15	86.0
16	288.0
17	806.0
18	1925.0
19	3867.0
20	6646.0
21	11124.0
22	16830.0
23	24919.0
24	34790.0
25	47526.0
26	62151.0
27	76298.0
28	92138.0
29	112942.0
30	139103.0
31	174199.0
32	222479.0
33	321132.0
34	479968.0
35	641849.0
36	328003.0
37	409204.0
38	527872.0
39	648993.0
40	438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.940395227860495	25.823794207526323	30.20973991830577	12.026070646307412
2	28.437471355260442	27.413944931870553	30.628585865357298	13.51999784751171
3	29.31378946931587	26.059975073091113	30.98502333306427	13.641212124528746
4	22.535891146113368	38.944692451834655	25.82429584674648	12.695120555305495
5	24.467247747297876	32.41752253158161	28.54507296798493	14.570156753135588
6	21.974055219533284	41.885233155068995	25.412586857690883	10.728124767706838
7	89.84928477648097	4.0339545907057195	4.279392980060753	1.8373676527525629
8	91.60340312046958	2.908504198492255	3.538722671493348	1.9493700095448263
9	88.0551037002277	4.539173690755838	4.965658235023447	2.440064373993016
10	57.908159891572964	22.814369318650808	9.315896354040545	9.961574435735683
11	48.93492872846771	19.447458080062532	18.293186234472554	13.324426956997208
12	45.25526823789191	20.908345498084422	19.684733431198584	14.151652832825082
13	22.55716520940473	40.130430757598354	20.29538797461341	17.017016058383508
14	15.916442411133472	41.04115676180026	24.31522826180642	18.727172565259842
15	15.293338094345568	23.7229063517102	44.51452952397631	16.469226029967928
16	17.129269234785852	19.074671734134863	43.05555745570412	20.74050157537517
17	17.89718767372108	21.5821381778913	25.42008864421056	35.100585504177054
18	22.738598994623796	25.36903545266783	29.658050785042576	22.234314767665794
19	31.636880697880482	26.1035492798969	20.885862940308126	21.373707081914493
20	34.63980707867664	24.08244486186908	21.940331382868838	19.33741667658544
21	25.004275334262733	29.621043491664352	23.804491130334533	21.57019004373838
22	25.392726504929065	26.363124774547376	22.124911814105275	26.11923690641829
23	24.069675863537714	30.505889472462517	22.183489593950416	23.240945070049357
24	22.027160571522124	23.564137538529312	35.564943809566806	18.843758080381757
25	19.569721239085354	26.659069112659505	31.249387202089007	22.52182244616613
26	19.19251134730718	37.095490673842846	22.76488945011677	20.94710852873321
27	20.12031588677729	33.709745162715805	26.213453872677583	19.956485077829324
28	17.38622252442185	28.760709427305862	35.791684737079486	18.061383311192802
29	17.826068913371923	24.824870907707048	34.15624967222438	23.192810506696656
30	21.139715187492218	30.68474665623257	27.295558075912606	20.879980080362603
31	31.107833734872724	26.792049109567586	21.29645464200973	20.80366251354996
32	32.901832396864116	26.54223277792751	21.57616411081484	18.979770714393535
33	29.237357893589554	27.49974804030078	22.138068442743126	21.124825623366537
34	20.838389628654728	26.968626115064183	26.726448380640193	25.4665358756409
35	23.155461186577412	24.687832193472214	27.981982943354446	24.17472367659593
36	29.97125607268479	26.47341699763454	23.203162516058157	20.352164413622514
37	22.559262973416313	32.48369330507737	26.408044286534498	18.548999434971826
38	22.119462188031708	32.38170093090558	22.796492720986897	22.70234416007581
39	22.518402178755945	31.48296866442206	25.02176430162016	20.97686485520184
40	26.331931935766466	25.386820843200802	23.805745228384936	24.475501992647793
41	17.96443013100536	23.871117939484982	27.245667775289345	30.91878415422031
42	22.07705087214539	25.937506698023675	22.7849550189232	29.200487410907733
43	22.441833792333217	27.55344623864072	24.24579683337962	25.758923135646437
44	21.21448223307891	29.87719871890464	25.56620246797376	23.342116580042692
45	18.62413130908227	34.90125231951135	21.53471046980337	24.93990590160301
46	21.58731418257205	33.11735484727138	23.40639480557148	21.888936164585093
47	22.257367370010456	26.010107574250586	26.784592926613378	24.947932129125583
48	23.699808145800073	21.949018862090714	30.90528549884144	23.445887493267772
49	21.03535142791604	24.811281045197237	30.266835582273167	23.886531944613555
50	19.654566672640733	31.61665551659491	25.818025356494473	22.910752454269883
51	17.51553143430966	32.79254345221727	24.050613573171606	25.641311540301466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	671.0
1	1589.5
2	2508.0
3	6974.5
4	11441.0
5	8557.5
6	5674.0
7	6108.5
8	6543.0
9	7051.0
10	7559.0
11	8026.0
12	8493.0
13	8450.5
14	8408.0
15	7780.5
16	7153.0
17	7619.5
18	8086.0
19	7718.5
20	7351.0
21	7779.5
22	8208.0
23	9664.5
24	11121.0
25	15175.5
26	27037.0
27	34844.0
28	43112.0
29	51380.0
30	56806.0
31	62232.0
32	72685.0
33	83138.0
34	92214.0
35	101290.0
36	105452.0
37	109614.0
38	139673.0
39	169732.0
40	239138.0
41	308544.0
42	365938.0
43	423332.0
44	422574.5
45	421817.0
46	400727.5
47	379638.0
48	362033.5
49	344429.0
50	315888.0
51	287347.0
52	272653.5
53	257960.0
54	240322.5
55	222685.0
56	212181.0
57	201677.0
58	201842.0
59	202007.0
60	187098.0
61	172189.0
62	157145.5
63	142102.0
64	119002.5
65	95903.0
66	81255.0
67	66607.0
68	55340.0
69	44073.0
70	39410.0
71	34747.0
72	30280.0
73	25813.0
74	19076.5
75	10345.5
76	8351.0
77	6349.0
78	4347.0
79	3719.0
80	3091.0
81	2185.5
82	1280.0
83	904.0
84	528.0
85	304.5
86	81.0
87	53.5
88	26.0
89	21.5
90	17.0
91	11.0
92	5.0
93	5.5
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4385622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.959423842352715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10449903007017	11.534388811430935
2	9.927880428506063	2.9703074237244094
3	3.5026470196339745	1.5719274401057446
4	1.8626391479642517	1.1145603391902388
5	1.046781688137445	0.782962547163076
6	0.7276965986388806	0.6531553108606467
7	0.4943313450358712	0.5176438476266327
8	0.3691126060221366	0.4417369535232395
9	0.26332461069168756	0.35452660135135294
>10	2.077200541325155	6.851481095000494
>50	0.7446103856828774	8.151119627469807
>100	1.8370494840243878	60.06919522767963
>500	0.03640841741939055	3.4662055901202367
>1k	0.005652448366480723	1.3898993486234854
>5k	1.6624848136708012E-4	0.13088983613014485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5263	0.1200057825321015	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05632040335441586	0.0
2	0.0	0.0	0.0	0.17329354878281802	0.0
3	0.0	0.0	0.0	0.25734091994248476	0.0
4	0.0	0.0	0.0	0.4606644165867464	0.0
5	0.0	0.0	0.0	0.8021895183853054	0.0
6	0.0	0.0	0.0	1.1752950892712595	0.0
7	0.0	0.0	0.0	1.3668528660244772	0.0
8	2.280178273458132E-5	0.0	0.0	1.8448922410549746	0.0
9	2.280178273458132E-5	0.0	0.0	1.9791947413616586	0.0
10	2.280178273458132E-5	0.0	0.0	2.2099487826356214	0.0
11	2.280178273458132E-5	0.0	0.0	2.582119480429458	0.0
12	2.280178273458132E-5	0.0	0.0	2.8837414624424995	0.0
13	2.280178273458132E-5	0.0	0.0	2.9990500777312774	0.0
14	2.280178273458132E-5	0.0	0.0	3.0429435094953465	0.0
15	2.280178273458132E-5	0.0	0.0	3.108703850901879	0.0
16	2.280178273458132E-5	0.0	0.0	3.2673586551690956	0.0
17	2.280178273458132E-5	0.0	0.0	3.4479259726442453	0.0
18	4.560356546916264E-5	0.0	0.0	3.6580672023261465	0.0
19	6.840534820374396E-5	0.0	0.0	3.7981157518819453	0.0
20	6.840534820374396E-5	0.0	0.0	3.938779949571577	0.0
21	6.840534820374396E-5	0.0	0.0	4.130679753065814	0.0
22	6.840534820374396E-5	0.0	0.0	4.338335588429646	0.0
23	6.840534820374396E-5	0.0	0.0	4.560014520175246	0.0
24	9.120713093832528E-5	0.0	0.0	4.73025263007163	0.0
25	9.120713093832528E-5	0.0	0.0	4.873789852385819	0.0
26	9.120713093832528E-5	0.0	0.0	5.005150922719742	0.0
27	9.120713093832528E-5	0.0	0.0	5.130537925977205	0.0
28	9.120713093832528E-5	0.0	0.0	5.269697206006354	0.0
29	1.140089136729066E-4	0.0	0.0	5.425205364256199	0.0
30	1.140089136729066E-4	0.0	0.0	5.61518981800073	0.0
31	1.3681069640748792E-4	0.0	0.0	5.80202762572789	0.0
32	1.5961247914206923E-4	0.0	0.0	5.960021178295804	0.0
33	1.5961247914206923E-4	0.0	0.0	6.121754223232189	0.0
34	1.5961247914206923E-4	0.0	0.0	6.298650453687071	0.0
35	1.5961247914206923E-4	0.0	0.0	6.51873326064125	0.0
36	1.5961247914206923E-4	0.0	0.0	6.715945879512644	0.0
37	2.0521604461123187E-4	0.0	0.0	6.912702462729346	0.0
38	2.0521604461123187E-4	0.0	0.0	7.0917648625440135	0.0
39	2.0521604461123187E-4	0.0	0.0	7.2964792679350845	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	65	0.0	45.000004	25
TACGAGT	35	1.2129749E-7	45.0	44
CGTAAGT	25	3.8926097E-5	45.0	22
ACGTATG	280	0.0	44.196426	1
GCGATAT	520	0.0	44.134617	9
TAGTACG	130	0.0	43.269234	1
TCGACAG	125	0.0	43.2	1
TTACGAG	85	0.0	42.35294	1
CGGTCTA	225	0.0	42.0	31
ACTTACG	60	3.6379788E-12	41.249996	1
ATCGATC	55	6.184564E-11	40.909092	11
CGCTAGC	50	1.0822987E-9	40.5	13
AACGCGC	50	1.0822987E-9	40.5	41
TCGTAAG	50	1.0822987E-9	40.5	1
CGATATA	140	0.0	40.17857	10
TTCGTTA	45	1.9304935E-8	40.000004	42
GTTAGCG	310	0.0	39.919353	1
TATAGCG	220	0.0	39.886368	1
ATAGCGG	965	0.0	39.637306	2
CGGGTAT	455	0.0	39.56044	6
>>END_MODULE