FastQCFastQC Report
Thu 26 May 2016
SRR1545321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545321_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2657148
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA302.166582E-645.00000424
ACCGGTT351.2127384E-745.00000430
ACCGGTA302.166582E-645.00000418
GTCGATC351.2127384E-745.00000422
CGAGTCG302.166582E-645.00000441
CGAAACG351.2127384E-745.00000430
CTATCCG351.2127384E-745.00000414
GCTATGC351.2127384E-745.00000421
CGTTCGA600.045.00000419
TGCATAA351.2127384E-745.0000041
GTTACAC302.166582E-645.00000412
ACGATCC302.166582E-645.00000444
CGCTAAG600.045.00000415
GCTTAGC302.166582E-645.00000422
TCGCTAA600.045.00000414
GTCGTGC302.166582E-645.00000437
TATAGTC700.045.00000412
TTACGTA351.2127384E-745.00000440
CCGGTAC600.045.00000431
ACTCCGA302.166582E-645.00000421
Produced by FastQC (version 0.11.5)