##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545321_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2657148
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28864293595991	31.0	31.0	33.0	30.0	34.0
2	31.59330191618984	31.0	31.0	34.0	30.0	34.0
3	31.667980104984743	31.0	31.0	34.0	30.0	34.0
4	30.26613760317453	35.0	33.0	37.0	0.0	37.0
5	32.81970518766738	35.0	33.0	37.0	19.0	37.0
6	34.42284697728542	35.0	33.0	37.0	30.0	37.0
7	35.484085192093175	36.0	35.0	37.0	33.0	37.0
8	35.701923641438114	37.0	35.0	37.0	35.0	37.0
9	37.77363172845472	39.0	37.0	39.0	35.0	39.0
10	37.249623280299026	39.0	37.0	39.0	34.0	39.0
11	36.65450776546884	39.0	35.0	39.0	32.0	39.0
12	34.83182156206579	35.0	34.0	39.0	30.0	39.0
13	34.044230505790416	35.0	33.0	39.0	25.0	39.0
14	34.62407777060216	35.0	33.0	40.0	25.0	41.0
15	35.20698470691132	35.0	33.0	40.0	30.0	41.0
16	35.57553361724676	35.0	34.0	40.0	31.0	41.0
17	35.548159906787276	35.0	34.0	40.0	31.0	41.0
18	35.55374145512407	36.0	34.0	39.0	31.0	41.0
19	35.52552210114002	36.0	34.0	39.0	31.0	41.0
20	35.34330304521991	35.0	34.0	39.0	30.0	41.0
21	35.10610210646904	35.0	34.0	39.0	30.0	41.0
22	34.938253721659464	35.0	34.0	39.0	29.0	41.0
23	35.00346010082991	35.0	34.0	39.0	30.0	41.0
24	34.91516166957956	35.0	34.0	39.0	30.0	41.0
25	34.78621213421307	35.0	34.0	39.0	29.0	41.0
26	34.61315139390053	35.0	34.0	38.0	29.0	41.0
27	34.581412100492706	35.0	34.0	39.0	29.0	41.0
28	34.6965065551486	35.0	34.0	38.0	29.0	41.0
29	34.857357587910045	35.0	34.0	38.0	29.0	41.0
30	34.8581757583695	35.0	34.0	38.0	29.0	41.0
31	34.612737039863795	35.0	34.0	38.0	29.0	41.0
32	34.42570304702636	35.0	34.0	38.0	29.0	41.0
33	34.262241320393144	35.0	33.0	38.0	27.0	41.0
34	34.260382936893244	35.0	34.0	38.0	27.0	41.0
35	34.12640432523894	35.0	33.0	38.0	27.0	41.0
36	33.85951892781283	35.0	33.0	38.0	25.0	41.0
37	33.76639276397099	35.0	33.0	38.0	25.0	41.0
38	33.83881891411393	35.0	33.0	38.0	27.0	41.0
39	33.83050925277779	35.0	33.0	38.0	26.0	41.0
40	33.664896723855804	35.0	33.0	38.0	24.0	41.0
41	33.769946197953594	35.0	33.0	38.0	25.0	41.0
42	33.72820783787731	35.0	33.0	38.0	26.0	40.0
43	33.58739106741514	35.0	33.0	38.0	26.0	40.0
44	33.53863653812283	35.0	33.0	37.0	26.0	40.0
45	33.43469765327336	35.0	33.0	37.0	25.0	40.0
46	33.42499552151404	35.0	33.0	37.0	25.0	40.0
47	33.360881290767395	35.0	33.0	37.0	25.0	40.0
48	33.336703488100774	35.0	33.0	37.0	24.0	40.0
49	33.39650030784887	35.0	33.0	37.0	24.0	40.0
50	33.13687344476107	35.0	32.0	36.0	24.0	40.0
51	33.03545304966077	35.0	32.0	36.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	4.0
14	14.0
15	69.0
16	241.0
17	667.0
18	1572.0
19	3012.0
20	5517.0
21	8615.0
22	13145.0
23	18519.0
24	26021.0
25	35113.0
26	43973.0
27	53601.0
28	63725.0
29	76998.0
30	94002.0
31	118165.0
32	154226.0
33	239016.0
34	379438.0
35	543344.0
36	143053.0
37	166124.0
38	212925.0
39	255862.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.874060458807715	26.291610403334705	31.67237955883526	12.16194957902232
2	27.221517205665624	27.44736085457039	31.516874483468744	13.814247456295245
3	27.509532777248385	25.840261814547027	31.370476917356505	15.279728490848083
4	21.55431312068428	38.506022246408556	26.825867433804966	13.113797199102194
5	23.733529332953978	33.09145745739417	29.07094373365729	14.104069475994564
6	22.290365459507715	40.057271932161854	26.404212335933114	11.248150272397323
7	89.0704996484953	4.858705649817022	4.410443076561787	1.660351625125887
8	92.49198764991637	2.223286019446414	3.1753218112050967	2.109404519432113
9	90.61162569792876	2.999230754177035	4.496625705455624	1.8925178424385847
10	73.27988504968485	12.608179898146435	6.633841999015486	7.47809305315323
11	67.47904143841443	14.02300511676429	10.702490038191325	7.795463406629965
12	58.0470489411956	20.389605697537363	12.458169435801093	9.105175925465952
13	19.464628993191198	57.14822810020368	14.344063635145652	9.043079271459474
14	10.451544287333638	56.82724485049384	22.77934838405689	9.941862478115633
15	8.567908148134768	20.31633164580972	61.70077842860089	9.414981777454624
16	11.178225676552454	13.290791480188533	58.48974163275813	17.041241210500882
17	12.348578250063602	13.887634411030172	25.06111816127668	48.702669177629545
18	21.927043582066187	21.39350160397539	35.801882318937444	20.877572495020978
19	35.831462906845985	22.108252908757812	21.19712563997188	20.863158544424323
20	39.52263855833397	22.055075592326812	20.035165523335547	18.387120326003668
21	21.93818334545159	31.053558175908908	24.745065009551595	22.26319346908791
22	22.218860221560863	25.66383204849711	20.718492157757115	31.398815572184912
23	18.71969495112805	34.634088880258076	19.878606686567704	26.76760948204616
24	20.757255523591457	22.802305328871405	42.05095086912735	14.389488278409784
25	13.674511167612794	23.915679518039642	41.20011380623134	21.209695508116223
26	13.374565511593634	42.13224103437219	24.669645800685547	19.823547653348626
27	16.425167134085118	40.408475553488174	27.064770197218973	16.10158711520773
28	11.349951150632181	29.579647050145496	45.56498170218595	13.505420097036373
29	11.275359897152887	24.186646735522448	43.13662618717512	21.401367180149546
30	16.958031694132206	33.321741957918796	32.490361846611485	17.229864501337524
31	35.96480135844898	25.856745653610563	20.354681033950687	17.823771953989766
32	38.53880175285682	25.12366642731229	21.57151201212729	14.766019807703598
33	33.137521884366244	27.35876962818782	20.970491670016123	18.533216817429814
34	17.250676289013633	27.98052648930357	26.526825001844085	28.24197221983871
35	17.73582051131514	24.37553346670942	33.180462661470116	24.708183360505323
36	38.61994138075862	19.949886118499986	25.219370543153786	16.21080195758761
37	19.793778893761278	34.57861586934563	28.46781586874348	17.15978936814961
38	18.44323312062407	37.53008865144132	20.3395143966388	23.68716383129581
39	19.388156022923827	36.12154836689563	25.520520497917314	18.969775112263225
40	26.93448012681266	24.754398324820446	21.153168735802446	27.15795281256445
41	14.994874203469283	21.192157907651364	25.38586484456267	38.42710304431669
42	23.38270205498527	23.981238530936178	19.465569851585233	33.17048956249332
43	22.49012851372976	25.913874575296518	22.679993737646527	28.916003173327194
44	18.640098330992476	32.5094048205068	27.865177250194566	20.985319598306155
45	14.458434381524853	44.04383948504186	18.304738765021746	23.192987368411544
46	22.917428761965837	35.976806711556904	22.427655516365665	18.678109010111594
47	20.639911664687098	26.44929827017539	26.13979349287281	26.7709965722647
48	25.143537356594365	18.464684692008124	33.497042693895864	22.89473525750165
49	20.408799208775726	18.850963514264166	34.90099158947864	25.839245687481466
50	17.97434693137153	37.61374977983913	23.94066871698528	20.471234571804054
51	15.590964447595693	40.4146099502173	19.611515805668333	24.382909796518675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	601.0
1	737.0
2	873.0
3	2499.5
4	4126.0
5	3267.0
6	2408.0
7	2994.0
8	3580.0
9	4295.5
10	5011.0
11	5438.0
12	5865.0
13	6004.5
14	6144.0
15	6363.5
16	6583.0
17	6106.5
18	5630.0
19	5690.0
20	5750.0
21	5604.0
22	5458.0
23	5662.0
24	5866.0
25	5724.5
26	6855.0
27	8127.0
28	10273.5
29	12420.0
30	17877.0
31	23334.0
32	27392.5
33	31451.0
34	31778.0
35	32105.0
36	36254.0
37	40403.0
38	73568.5
39	106734.0
40	182585.5
41	258437.0
42	319423.5
43	380410.0
44	378896.0
45	377382.0
46	331613.0
47	285844.0
48	253323.5
49	220803.0
50	202612.0
51	184421.0
52	172136.5
53	159852.0
54	136829.0
55	113806.0
56	101441.5
57	89077.0
58	84126.0
59	79175.0
60	70665.5
61	62156.0
62	54408.0
63	46660.0
64	36552.0
65	26444.0
66	23892.0
67	21340.0
68	16376.5
69	11413.0
70	10308.5
71	9204.0
72	8230.0
73	7256.0
74	5523.5
75	2292.0
76	793.0
77	664.5
78	536.0
79	341.0
80	146.0
81	115.0
82	84.0
83	53.0
84	22.0
85	30.0
86	38.0
87	22.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2657148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.94212791367283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2807796900434	10.914006436206273
2	10.083690500597623	2.811762056024393
3	3.5894031656817385	1.5013175420903093
4	1.6954023524078217	0.9455006584964669
5	0.9748000564666204	0.679539353875656
6	0.6358456549018362	0.5319024872396684
7	0.40405874474315867	0.39434070926930387
8	0.26923994586344036	0.3003022211758747
9	0.20195493915150273	0.2534113435003438
>10	1.4183076525140268	4.398196608206094
>50	0.5225800513484053	5.3355163303332755
>100	1.8260999989818203	62.35037996898218
>500	0.08817069292480265	7.670097931290668
>1k	0.009666554373815571	1.9137263533094937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07910737377067442	0.0
2	0.0	0.0	0.0	0.2823327868827781	0.0
3	0.0	0.0	0.0	0.40833254301228233	0.0
4	0.0	0.0	0.0	0.7090308857466727	0.0
5	0.0	0.0	0.0	1.1075408671252034	0.0
6	0.0	0.0	0.0	1.5637442852261145	0.0
7	0.0	0.0	0.0	1.7665180863090804	0.0
8	0.0	0.0	0.0	2.248312852727812	0.0
9	0.0	0.0	0.0	2.3838340958049757	0.0
10	0.0	0.0	0.0	2.5799089851223944	0.0
11	0.0	0.0	0.0	2.9141771553560436	0.0
12	0.0	0.0	0.0	3.1659132272647215	0.0
13	3.7634335761500674E-5	0.0	0.0	3.273622696214136	0.0
14	3.7634335761500674E-5	0.0	0.0	3.3181441154199915	0.0
15	3.7634335761500674E-5	0.0	0.0	3.3847568897178477	0.0
16	3.7634335761500674E-5	0.0	0.0	3.545380234747933	0.0
17	3.7634335761500674E-5	0.0	0.0	3.71910032862302	0.0
18	3.7634335761500674E-5	0.0	0.0	3.9377181850615774	0.0
19	7.526867152300135E-5	0.0	0.0	4.0549491409586516	0.0
20	7.526867152300135E-5	0.0	0.0	4.172029559512681	0.0
21	7.526867152300135E-5	0.0	0.0	4.340932458410296	0.0
22	7.526867152300135E-5	0.0	0.0	4.481873045837116	0.0
23	7.526867152300135E-5	0.0	0.0	4.665904947710854	0.0
24	1.1290300728450203E-4	0.0	0.0	4.779861716396678	0.0
25	1.505373430460027E-4	0.0	0.0	4.8709368089395095	0.0
26	1.505373430460027E-4	0.0	0.0	4.971232313743909	0.0
27	1.505373430460027E-4	0.0	0.0	5.053576240390073	0.0
28	1.881716788075034E-4	0.0	0.0	5.15206529707792	0.0
29	1.881716788075034E-4	0.0	0.0	5.24863500264193	0.0
30	1.881716788075034E-4	0.0	0.0	5.391382038185302	0.0
31	1.881716788075034E-4	0.0	0.0	5.531795744911461	0.0
32	2.2580601456900407E-4	0.0	0.0	5.6422901547072275	0.0
33	2.2580601456900407E-4	0.0	0.0	5.75997272263344	0.0
34	2.2580601456900407E-4	0.0	0.0	5.891542360455646	0.0
35	2.2580601456900407E-4	0.0	0.0	6.072902224490318	0.0
36	2.2580601456900407E-4	0.0	0.0	6.222047097113145	0.0
37	2.2580601456900407E-4	0.0	0.0	6.35207372716913	0.0
38	2.2580601456900407E-4	0.0	0.0	6.476417572525128	0.0
39	3.010746860920054E-4	0.0	0.0	6.6025678659976785	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	30	2.166582E-6	45.000004	24
ACCGGTT	35	1.2127384E-7	45.000004	30
ACCGGTA	30	2.166582E-6	45.000004	18
GTCGATC	35	1.2127384E-7	45.000004	22
CGAGTCG	30	2.166582E-6	45.000004	41
CGAAACG	35	1.2127384E-7	45.000004	30
CTATCCG	35	1.2127384E-7	45.000004	14
GCTATGC	35	1.2127384E-7	45.000004	21
CGTTCGA	60	0.0	45.000004	19
TGCATAA	35	1.2127384E-7	45.000004	1
GTTACAC	30	2.166582E-6	45.000004	12
ACGATCC	30	2.166582E-6	45.000004	44
CGCTAAG	60	0.0	45.000004	15
GCTTAGC	30	2.166582E-6	45.000004	22
TCGCTAA	60	0.0	45.000004	14
GTCGTGC	30	2.166582E-6	45.000004	37
TATAGTC	70	0.0	45.000004	12
TTACGTA	35	1.2127384E-7	45.000004	40
CCGGTAC	60	0.0	45.000004	31
ACTCCGA	30	2.166582E-6	45.000004	21
>>END_MODULE