FastQCFastQC Report
Thu 26 May 2016
SRR1545320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545320_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2268920
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.12353895245314953No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA207.0347404E-445.00000423
ATTACGT207.0347404E-445.00000445
CGACATA207.0347404E-445.00000445
CATTACG207.0347404E-445.00000444
CTAACGT207.0347404E-445.00000427
TCTACGA207.0347404E-445.00000440
CACTAGC207.0347404E-445.00000415
TACGACG302.16644E-645.0000041
TACGACA351.2126293E-745.00000414
CGCTAAT351.2126293E-745.00000422
TATTCCG302.16644E-645.0000041
GTCGTTA351.2126293E-745.00000434
CGTACTA351.2126293E-745.0000048
TTAACGA207.0347404E-445.0000041
CGTCGAT302.16644E-645.00000434
ACCCGTC207.0347404E-445.00000436
CCGAACA207.0347404E-445.00000413
GACGATA207.0347404E-445.00000421
ATACGGT302.16644E-645.00000432
ACGAGTT207.0347404E-445.00000443