FastQCFastQC Report
Thu 26 May 2016
SRR1545319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545319_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1982620
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT453.8562575E-1045.00000422
CTAGAGT453.8562575E-1045.00000437
CTATGCG453.8562575E-1045.0000041
ACACGAA650.045.00000410
GCGACAA351.2125201E-745.00000423
TCCGATT351.2125201E-745.00000417
ATCACGC351.2125201E-745.00000418
ACGGACG453.8562575E-1045.00000428
TCGTCGG453.8562575E-1045.00000417
CGAATGT453.8562575E-1045.00000416
TACTCCG453.8562575E-1045.00000414
GTTCACG351.2125201E-745.0000041
CGATGTC700.045.00000410
CGAGAAT453.8562575E-1045.00000442
ACCCGTT351.2125201E-745.00000432
GTCTTCG351.2125201E-745.00000414
AGTAGTC453.8562575E-1045.00000440
CGACTTG351.2125201E-745.00000429
ATCGGAA453.8562575E-1045.00000425
ACCCCGA351.2125201E-745.00000444