##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545319_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1982620
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289701001704813	31.0	31.0	33.0	30.0	34.0
2	31.58946091535443	31.0	31.0	34.0	30.0	34.0
3	31.66700527584711	31.0	31.0	34.0	30.0	34.0
4	30.27851529793909	35.0	33.0	37.0	0.0	37.0
5	32.825982286065916	35.0	33.0	37.0	19.0	37.0
6	34.43557867871806	35.0	33.0	37.0	30.0	37.0
7	35.487120577821265	36.0	35.0	37.0	33.0	37.0
8	35.70856089417034	37.0	35.0	37.0	35.0	37.0
9	37.77820913740404	39.0	37.0	39.0	35.0	39.0
10	37.242211820722076	39.0	37.0	39.0	34.0	39.0
11	36.66342768659652	39.0	35.0	39.0	32.0	39.0
12	34.820858258264316	35.0	34.0	39.0	30.0	39.0
13	34.08447155783761	35.0	33.0	39.0	25.0	39.0
14	34.7155420605058	35.0	33.0	40.0	25.0	41.0
15	35.29233539457889	35.0	33.0	40.0	30.0	41.0
16	35.643332055562844	35.0	34.0	40.0	31.0	41.0
17	35.6078123896662	35.0	34.0	40.0	31.0	41.0
18	35.5950373747869	36.0	34.0	39.0	31.0	41.0
19	35.557668136102734	36.0	34.0	39.0	31.0	41.0
20	35.387017683671104	35.0	34.0	39.0	30.0	41.0
21	35.144862353855	35.0	34.0	39.0	30.0	41.0
22	34.991245422723466	35.0	34.0	39.0	30.0	41.0
23	35.06425840554418	35.0	34.0	39.0	30.0	41.0
24	34.952317640294154	35.0	34.0	39.0	29.0	41.0
25	34.83793213021154	35.0	34.0	39.0	29.0	41.0
26	34.66217227708789	35.0	34.0	39.0	29.0	41.0
27	34.60641474412646	35.0	34.0	39.0	29.0	41.0
28	34.7599439126005	35.0	34.0	39.0	29.0	41.0
29	34.911400066578565	35.0	34.0	39.0	29.0	41.0
30	34.9065186470428	35.0	34.0	38.0	29.0	41.0
31	34.64633616124119	35.0	34.0	38.0	29.0	41.0
32	34.483757351383524	35.0	34.0	38.0	29.0	41.0
33	34.3298408166971	35.0	33.0	38.0	28.0	41.0
34	34.33516104952033	35.0	34.0	38.0	28.0	41.0
35	34.179258254229254	35.0	33.0	38.0	27.0	41.0
36	33.928456789500764	35.0	33.0	38.0	25.0	41.0
37	33.8292466534182	35.0	33.0	38.0	25.0	41.0
38	33.87416196749756	35.0	33.0	38.0	27.0	41.0
39	33.88458756594809	35.0	33.0	38.0	26.0	41.0
40	33.75507056319416	35.0	33.0	38.0	24.0	41.0
41	33.83269612936417	35.0	33.0	38.0	25.0	41.0
42	33.7890664877788	35.0	33.0	38.0	26.0	40.0
43	33.650051447075086	35.0	33.0	38.0	26.0	40.0
44	33.585954444119395	35.0	33.0	38.0	26.0	40.0
45	33.49754970695343	35.0	33.0	38.0	26.0	40.0
46	33.49477055613279	35.0	33.0	37.0	26.0	40.0
47	33.446238815305	35.0	33.0	37.0	26.0	40.0
48	33.42605945667854	35.0	33.0	37.0	26.0	40.0
49	33.46739163329332	35.0	33.0	37.0	25.0	40.0
50	33.202643976152764	35.0	32.0	36.0	24.0	40.0
51	33.10651965580898	35.0	32.0	36.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	49.0
16	134.0
17	508.0
18	1024.0
19	2064.0
20	3824.0
21	6174.0
22	9379.0
23	13603.0
24	18984.0
25	25494.0
26	33002.0
27	39911.0
28	47773.0
29	57130.0
30	69363.0
31	88204.0
32	114318.0
33	175825.0
34	279127.0
35	401832.0
36	109762.0
37	125816.0
38	162245.0
39	196916.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.198615972803662	25.098304264054633	31.164973620764442	12.53810614237726
2	26.888258970453236	26.456305292996134	32.48670950560369	14.168726230946929
3	27.978129949259063	26.279266828741765	31.624214423338813	14.118388798660359
4	21.273163793364336	39.9838597411506	25.550483703382394	13.192492762102672
5	23.393035478306484	32.92617849108755	30.033692790348127	13.64709324025784
6	21.419888833967175	41.05602687353098	26.118368623336796	11.405715669165044
7	90.17143981196598	4.258708174032341	4.016806044526939	1.5530459694747356
8	92.99406845487285	1.9092413069574603	3.242124058064581	1.8545661801051132
9	90.69317367927287	2.8113304617122794	4.485125742704098	2.0103701163107406
10	72.24879200250174	13.300985564555992	6.958872602919369	7.491349830022899
11	67.15568288426425	13.181497210761517	11.10676781228879	8.556052092685436
12	56.58033309459201	20.73705500801969	13.312636813912903	9.369975083475401
13	17.941763928539004	57.13258213878605	14.656817746214603	10.26883618646034
14	10.263237534171953	55.770092100352066	23.551008261794998	10.415662103680988
15	9.2490240187227	19.913952245009128	61.058145282504974	9.778878453763202
16	11.497160323208684	12.66677426839233	57.51445057550111	18.321614832897883
17	12.062069382937729	14.68476056934763	25.20195498885313	48.05121505886151
18	22.865097699004348	21.134710635421815	36.15690349133974	19.843288174234093
19	37.09490472203448	23.07451755757533	19.909967618605684	19.920610101784508
20	39.887825200996666	22.417558584095794	19.272276079127618	18.422340135779926
21	21.29994653539256	31.92502849764453	24.380970634816556	22.39405433214635
22	22.300339954202013	25.375563648102005	20.917271085735038	31.40682531196094
23	18.69087369238684	35.18884102853799	19.82250759096549	26.297777688109676
24	20.318870988893483	21.39300521532114	44.239440740030865	14.048683055754507
25	13.730568641494589	24.08984071582048	40.9467774964441	21.232813146240833
26	13.633827965015987	43.95158931111357	23.223764513623387	19.190818210247045
27	15.677184735350194	40.117016876658155	28.135850541203055	16.069947846788594
28	11.37444391764433	29.091000796925282	46.378327667429964	13.156227618000424
29	11.317045122111145	23.850359625142488	44.10915858813086	20.72343666461551
30	18.14387023231885	33.54112235324974	30.954141489544142	17.36086592488727
31	38.512725585336575	25.0587606298736	19.21296062785607	17.215553156933755
32	39.4621258738437	25.724294115866886	20.95620946020922	13.857370550080198
33	32.688412302912305	27.50310195599762	20.65484056450555	19.15364517658452
34	18.289384753507985	27.025804238835484	26.20633303406603	28.4784779735905
35	18.70227275019923	25.75178299421977	33.18422087944236	22.361723376138645
36	38.14583732636612	21.340498935751683	24.457334234497786	16.05632950338441
37	19.32917049157176	36.64050599711493	27.591167243344668	16.439156267968645
38	18.872451604442606	38.96233267091021	19.872794584943158	22.29242113970403
39	20.239834158840324	36.34453400046404	25.04826946162149	18.367362379074155
40	27.905902290907992	25.040602838668025	20.337280971643583	26.7162138987804
41	15.155400429734392	21.16457011429321	25.300057499672153	38.37997195630025
42	23.039916877666926	23.83008342496293	19.955664726473053	33.17433497089709
43	23.07925875861234	24.993695211386953	22.97565847212275	28.95138755787796
44	18.50899315047765	32.990134266778306	28.226639497230938	20.274233085513107
45	14.84540658320808	45.781844226326776	17.214594829064573	22.15815436140057
46	22.619362258022214	36.46008816616397	22.36111811643179	18.55943145938203
47	21.2624204335677	25.739677800082717	25.901383018430156	27.09651874791942
48	24.734946686707488	18.155975426455903	34.86568278338763	22.24339510344897
49	20.647627886332227	19.732979592660218	35.38630700789864	24.233085513108914
50	17.437633031039734	38.4669780391603	23.8044103257306	20.290978604069362
51	15.572979189153745	41.242446863241575	20.137696583308955	23.046877364295728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	502.0
1	706.5
2	911.0
3	2164.0
4	3417.0
5	2666.5
6	1916.0
7	2354.5
8	2793.0
9	3350.5
10	3908.0
11	4186.5
12	4465.0
13	4412.0
14	4359.0
15	4385.0
16	4411.0
17	4093.5
18	3776.0
19	3740.0
20	3704.0
21	3678.5
22	3653.0
23	3858.0
24	4063.0
25	4941.0
26	7332.0
27	8845.0
28	10078.5
29	11312.0
30	12787.0
31	14262.0
32	18128.0
33	21994.0
34	24800.5
35	27607.0
36	29400.0
37	31193.0
38	54871.0
39	78549.0
40	135808.5
41	193068.0
42	240268.0
43	287468.0
44	283577.0
45	279686.0
46	244962.5
47	210239.0
48	190803.5
49	171368.0
50	157559.0
51	143750.0
52	127341.0
53	110932.0
54	95753.5
55	80575.0
56	73124.0
57	65673.0
58	63361.0
59	61049.0
60	54180.0
61	47311.0
62	41693.0
63	36075.0
64	28150.5
65	20226.0
66	16609.0
67	12992.0
68	10250.0
69	7508.0
70	7160.5
71	6813.0
72	4797.0
73	2781.0
74	2487.5
75	1633.0
76	1072.0
77	663.0
78	254.0
79	161.5
80	69.0
81	50.0
82	31.0
83	25.5
84	20.0
85	12.0
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1982620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.121994591978199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16030760609927	11.819397489267857
2	10.268421605665068	3.1055803197803855
3	3.634930424944792	1.649021946846965
4	1.668568666004253	1.0092834537464237
5	0.843840613405627	0.6380276596205728
6	0.4725199879127429	0.42872668210908615
7	0.3328620505107121	0.3523476689388434
8	0.23465087209860366	0.2838711375102442
9	0.16139782169402828	0.21965912881327415
>10	1.4976385001914017	5.2501267104984155
>50	0.7290311185919163	8.087250491681887
>100	1.9500861916298375	62.146267947854106
>500	0.0399814494405083	3.9133912786157925
>1k	0.005763091811244439	1.0970480847161421
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08559381021073126	0.0
2	0.0	0.0	0.0	0.2947110389282868	0.0
3	0.0	0.0	0.0	0.4216642624406089	0.0
4	0.0	0.0	0.0	0.6931232409639769	0.0
5	0.0	0.0	0.0	1.0815990961455044	0.0
6	0.0	0.0	0.0	1.518647042801949	0.0
7	0.0	0.0	0.0	1.7187358142256206	0.0
8	0.0	0.0	0.0	2.2070290827289143	0.0
9	0.0	0.0	0.0	2.3351928256549415	0.0
10	0.0	0.0	0.0	2.5264044547114426	0.0
11	0.0	0.0	0.0	2.846385086400823	0.0
12	0.0	0.0	0.0	3.0726513401458675	0.0
13	5.043830890437905E-5	0.0	0.0	3.1796309933320557	0.0
14	5.043830890437905E-5	0.0	0.0	3.221595666340499	0.0
15	5.043830890437905E-5	0.0	0.0	3.2951347207230834	0.0
16	5.043830890437905E-5	0.0	0.0	3.4508377803109016	0.0
17	1.008766178087581E-4	0.0	0.0	3.6320121858954315	0.0
18	1.008766178087581E-4	0.0	0.0	3.8465767519746596	0.0
19	1.008766178087581E-4	0.0	0.0	3.9616769728944528	0.0
20	1.008766178087581E-4	0.0	0.0	4.08580565110813	0.0
21	1.5131492671313716E-4	0.0	0.0	4.255328807335748	0.0
22	1.5131492671313716E-4	0.0	0.0	4.397514400137192	0.0
23	2.017532356175162E-4	0.0	0.0	4.578133984323774	0.0
24	2.5219154452189526E-4	0.0	0.0	4.697874529662769	0.0
25	2.5219154452189526E-4	0.0	0.0	4.79083233297354	0.0
26	2.5219154452189526E-4	0.0	0.0	4.8974589179973975	0.0
27	2.5219154452189526E-4	0.0	0.0	4.987339984465001	0.0
28	3.026298534262743E-4	0.0	0.0	5.092402981912822	0.0
29	3.026298534262743E-4	0.0	0.0	5.194893625606521	0.0
30	3.026298534262743E-4	0.0	0.0	5.354429996671072	0.0
31	3.026298534262743E-4	0.0	0.0	5.507005881106818	0.0
32	3.026298534262743E-4	0.0	0.0	5.628410890639659	0.0
33	3.026298534262743E-4	0.0	0.0	5.7429058518525995	0.0
34	3.026298534262743E-4	0.0	0.0	5.874247208239602	0.0
35	3.026298534262743E-4	0.0	0.0	6.069998285097498	0.0
36	3.026298534262743E-4	0.0	0.0	6.201995339500257	0.0
37	3.026298534262743E-4	0.0	0.0	6.339187539720168	0.0
38	3.026298534262743E-4	0.0	0.0	6.458726331823547	0.0
39	3.026298534262743E-4	0.0	0.0	6.5885041006345135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	45	3.8562575E-10	45.000004	22
CTAGAGT	45	3.8562575E-10	45.000004	37
CTATGCG	45	3.8562575E-10	45.000004	1
ACACGAA	65	0.0	45.000004	10
GCGACAA	35	1.2125201E-7	45.000004	23
TCCGATT	35	1.2125201E-7	45.000004	17
ATCACGC	35	1.2125201E-7	45.000004	18
ACGGACG	45	3.8562575E-10	45.000004	28
TCGTCGG	45	3.8562575E-10	45.000004	17
CGAATGT	45	3.8562575E-10	45.000004	16
TACTCCG	45	3.8562575E-10	45.000004	14
GTTCACG	35	1.2125201E-7	45.000004	1
CGATGTC	70	0.0	45.000004	10
CGAGAAT	45	3.8562575E-10	45.000004	42
ACCCGTT	35	1.2125201E-7	45.000004	32
GTCTTCG	35	1.2125201E-7	45.000004	14
AGTAGTC	45	3.8562575E-10	45.000004	40
CGACTTG	35	1.2125201E-7	45.000004	29
ATCGGAA	45	3.8562575E-10	45.000004	25
ACCCCGA	35	1.2125201E-7	45.000004	44
>>END_MODULE