FastQCFastQC Report
Thu 26 May 2016
SRR1545318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545318_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3094252
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAT551.8189894E-1245.00000434
TCACGCA207.0351403E-445.017
GTCGATC207.0351403E-445.06
ACGCATT207.0351403E-445.017
CTATCGT253.892342E-545.033
ACGTTAC700.045.032
CGCATTA351.2128294E-745.011
CGTTGTA453.8562575E-1045.036
TAGCGGA351.2128294E-745.03
CGCGCGA351.2128294E-745.044
AATGCGT351.2128294E-745.037
TACGCAC207.0351403E-445.036
CTACGCA207.0351403E-445.035
TACGACC351.2128294E-745.036
CGTAAGT207.0351403E-445.019
ACGTAAC502.1827873E-1145.016
CACGTTA700.045.031
CGACTCG351.2128294E-745.02
CGGTAAT700.045.018
TGTACGA253.892342E-545.022
Produced by FastQC (version 0.11.5)