##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545318_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3094252
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29319880862968	31.0	31.0	33.0	30.0	34.0
2	31.61538426734474	31.0	31.0	34.0	30.0	34.0
3	31.713060539348444	31.0	31.0	34.0	30.0	34.0
4	30.25965112085247	35.0	33.0	37.0	0.0	37.0
5	32.84233701715309	35.0	33.0	37.0	19.0	37.0
6	34.48360346862505	35.0	33.0	37.0	30.0	37.0
7	35.51184971359799	36.0	35.0	37.0	33.0	37.0
8	35.71445473736463	37.0	35.0	37.0	35.0	37.0
9	37.783643995382405	39.0	37.0	39.0	35.0	39.0
10	37.19909399751539	39.0	37.0	39.0	33.0	39.0
11	36.695093353741065	39.0	35.0	39.0	32.0	39.0
12	35.39943546938	35.0	35.0	39.0	31.0	39.0
13	34.9007524274041	35.0	34.0	39.0	30.0	39.0
14	35.65812351418049	36.0	34.0	40.0	30.0	41.0
15	36.04299876028197	37.0	35.0	40.0	31.0	41.0
16	36.27914177642933	36.0	35.0	40.0	32.0	41.0
17	36.22405124081684	36.0	35.0	40.0	31.0	41.0
18	36.22064880300635	36.0	35.0	40.0	31.0	41.0
19	36.19951671680264	36.0	35.0	40.0	31.0	41.0
20	36.080709974494646	36.0	34.0	40.0	31.0	41.0
21	35.8998743476614	36.0	34.0	40.0	31.0	41.0
22	35.72600324731147	36.0	34.0	40.0	30.0	41.0
23	35.70727044856075	35.0	34.0	40.0	30.0	41.0
24	35.65143886147605	35.0	34.0	40.0	30.0	41.0
25	35.53433689305202	35.0	34.0	40.0	30.0	41.0
26	35.38371438396097	35.0	34.0	40.0	30.0	41.0
27	35.359238678685514	35.0	34.0	40.0	30.0	41.0
28	35.444537484341936	36.0	34.0	40.0	30.0	41.0
29	35.53634157786761	36.0	34.0	40.0	30.0	41.0
30	35.493983683294054	36.0	34.0	40.0	30.0	41.0
31	35.339170500657346	35.0	34.0	40.0	30.0	41.0
32	35.22516459551452	35.0	34.0	40.0	29.0	41.0
33	35.09622584068783	35.0	34.0	40.0	29.0	41.0
34	35.03703027419874	35.0	34.0	40.0	29.0	41.0
35	34.913762679962716	35.0	34.0	40.0	29.0	41.0
36	34.72868984168064	35.0	33.0	40.0	27.0	41.0
37	34.65962468473803	35.0	33.0	40.0	27.0	41.0
38	34.66431499438313	35.0	33.0	40.0	27.0	41.0
39	34.654756626157145	35.0	33.0	40.0	27.0	41.0
40	34.557246468613414	35.0	33.0	40.0	27.0	41.0
41	34.5899109057698	35.0	34.0	40.0	27.0	41.0
42	34.530906338591684	35.0	33.0	40.0	27.0	41.0
43	34.42875628746463	35.0	33.0	39.0	27.0	41.0
44	34.36761129991998	35.0	33.0	39.0	27.0	41.0
45	34.261729813861315	35.0	33.0	39.0	27.0	41.0
46	34.22533523449286	35.0	33.0	39.0	27.0	41.0
47	34.17293904956674	35.0	33.0	39.0	27.0	41.0
48	34.1153677851707	35.0	33.0	39.0	27.0	41.0
49	34.10623795346985	35.0	33.0	39.0	26.0	41.0
50	33.91772131035223	35.0	33.0	39.0	26.0	40.0
51	33.83251913548089	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	9.0
15	44.0
16	191.0
17	565.0
18	1269.0
19	2688.0
20	4808.0
21	7636.0
22	11702.0
23	16932.0
24	24628.0
25	33573.0
26	44103.0
27	54119.0
28	65391.0
29	80016.0
30	98571.0
31	124597.0
32	160755.0
33	237653.0
34	366845.0
35	515795.0
36	211798.0
37	259214.0
38	343733.0
39	427388.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.982270351606786	25.321208485928103	32.24844001070372	12.448081151761395
2	27.119025858268813	26.776810679931696	32.33999687161873	13.76416659018076
3	28.12046336238936	26.080842801426645	31.50538482321414	14.293309012969853
4	21.691995351380562	39.489414566105154	25.781739819510495	13.036850263003789
5	23.022882428451204	32.619159654740464	29.798639541963617	14.559318374844713
6	21.66892030771896	40.611947572466626	26.22672620071022	11.492405919104197
7	90.77531500343217	3.823347290395223	3.85783058393434	1.5435071222382661
8	93.282673809373	2.0800826823413217	3.06002872422802	1.5772147840576658
9	90.5560374526703	3.5370745498427407	4.028728106178812	1.8781598913081416
10	63.22949779138868	21.311257130964123	7.093927708538283	8.365317369108915
11	53.90679233624153	17.833906223539646	16.12363828156207	12.13566315865676
12	47.07056826657945	21.28100749389513	18.25875849801503	13.389665741510386
13	20.479812245415047	45.37429401354511	20.272169170448947	13.87372457059089
14	13.559626042093534	45.654749516199715	25.889875808434475	14.895748633272273
15	12.889350964304136	22.324813880705253	51.07651219099155	13.709322963999055
16	15.488816036961436	17.378254906193806	48.06476654131596	19.068162515528794
17	16.02367874368345	18.659663143144126	25.899215707059415	39.41744240611301
18	23.079762087897173	23.190386561921912	32.60206343891836	21.127787911262562
19	32.880159728425475	24.26143701288712	21.651468594025307	21.20693466466209
20	34.95041774231705	23.816127451804185	21.85665550187897	19.376799303999803
21	24.282443705296142	29.4252698228845	25.681101603877128	20.611184867942235
22	25.065637834281112	24.75926330499261	22.307329849023287	27.867769011702993
23	21.571029121092916	31.650848088649536	22.19719014482337	24.580932645434178
24	20.953109184384466	22.687324755708328	39.13168675337367	17.227879306533534
25	17.111114414727695	25.766291821092786	35.529814636946185	21.592779127233335
26	17.351915745711725	37.693827134958624	24.390256514336905	20.564000604992742
27	18.423741828396654	35.875132342162175	27.45060841844814	18.250517410993027
28	15.285826752313644	28.10217138099935	39.64253719477276	16.969464671914245
29	15.462024424642854	24.69546759604583	37.703684121396705	22.13882385791461
30	19.628330207106597	32.109440342932636	29.30879579297355	18.953433656987215
31	32.95006353716504	25.72605592563243	22.159604324405382	19.164276212797148
32	34.254223637893745	25.769038850100124	23.399774808257376	16.57696270374876
33	30.509150515213367	26.581157578632897	22.64010817477051	20.26958373138322
34	19.479990640710582	26.711754569440373	27.145817470587396	26.66243731926165
35	20.870472088246206	24.213250892299655	31.517213206939836	23.3990638125143
36	33.49316733090905	23.222284416395304	24.61858310182881	18.665965150866835
37	21.042403786116967	33.082631925260124	27.518831691794983	18.356132596827926
38	20.711305995762466	34.79574385021	21.756792917965313	22.736157236062223
39	21.531924355223815	32.59492116349929	25.009856986438077	20.863297494838818
40	25.67544595592085	24.551846455944766	22.87105251931646	26.901655068817927
41	17.441113393479263	22.769994169834906	26.006866926158565	33.78202551052726
42	22.642305797976377	24.35871415773505	22.313276358874454	30.68570368541412
43	22.22740746390404	25.40158332288385	24.98355014394432	27.387459069267788
44	19.554128105920267	30.979700425175455	27.0430462677248	22.423125201179477
45	17.183442072591372	39.019607969874464	20.912388519099284	22.88456143843488
46	22.46659289547199	33.42681850088486	24.39403771897053	19.712550884672613
47	21.87672497262666	25.516441453378718	26.59356768614838	26.013265887846238
48	23.207676685673952	21.356987084439147	32.50468934010546	22.93064688978144
49	21.191777528139273	21.98977329577552	33.24153947383729	23.57690970224791
50	19.115734594338146	34.05579118959929	25.074299055151293	21.754175160911263
51	17.506137185982265	35.06896012348057	23.187768804867865	24.237133885669298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	332.0
1	764.5
2	1197.0
3	3657.0
4	6117.0
5	4668.0
6	3219.0
7	3578.0
8	3937.0
9	4370.5
10	4804.0
11	4998.5
12	5193.0
13	5259.5
14	5326.0
15	4890.0
16	4454.0
17	4705.5
18	4957.0
19	4691.5
20	4426.0
21	5708.0
22	6990.0
23	7971.0
24	8952.0
25	10714.0
26	14331.0
27	16186.0
28	19364.0
29	22542.0
30	26936.5
31	31331.0
32	40788.0
33	50245.0
34	56853.5
35	63462.0
36	69913.0
37	76364.0
38	107707.0
39	139050.0
40	202249.0
41	265448.0
42	311353.0
43	357258.0
44	350336.0
45	343414.0
46	323367.5
47	303321.0
48	276857.5
49	250394.0
50	237160.0
51	223926.0
52	197443.0
53	170960.0
54	162606.5
55	154253.0
56	141497.5
57	128742.0
58	123546.0
59	118350.0
60	111646.5
61	104943.0
62	89057.5
63	73172.0
64	59650.5
65	46129.0
66	38956.5
67	31784.0
68	26197.5
69	20611.0
70	16594.5
71	12578.0
72	10705.5
73	8833.0
74	6532.5
75	3252.5
76	2273.0
77	1723.5
78	1174.0
79	967.0
80	760.0
81	435.0
82	110.0
83	63.5
84	17.0
85	11.5
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3094252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.19523580258257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88478119800006	11.0559283453816
2	9.729727340295764	2.7623154778066583
3	3.4890848338598373	1.4858514585556504
4	1.6642876791497765	0.9449982419545582
5	0.9502300891725377	0.6744370091256618
6	0.61897105450026	0.5271864044162627
7	0.39346221367445655	0.39097022317605273
8	0.2971335324950167	0.3374304446896873
9	0.2134625197506337	0.2727135722586316
>10	1.7919377551932483	5.950564930685146
>50	0.8398374861116383	8.816501596479904
>100	2.0829611181105854	62.38575119019546
>500	0.040157051175052465	3.5871275596021444
>1k	0.003966128511116293	0.8082235456725978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.231798832157174E-5	0.0	0.0	0.052872228894091364	0.0
2	3.231798832157174E-5	0.0	0.0	0.17965569707961732	0.0
3	3.231798832157174E-5	0.0	0.0	0.2637147847040254	0.0
4	3.231798832157174E-5	0.0	0.0	0.475042110338783	0.0
5	3.231798832157174E-5	0.0	0.0	0.7880418272332053	0.0
6	3.231798832157174E-5	0.0	0.0	1.1070850079437615	0.0
7	3.231798832157174E-5	0.0	0.0	1.275558681064115	0.0
8	3.231798832157174E-5	0.0	0.0	1.6923314584591043	0.0
9	3.231798832157174E-5	0.0	0.0	1.8083853545218682	0.0
10	3.231798832157174E-5	0.0	0.0	1.9866190601153364	0.0
11	3.231798832157174E-5	0.0	0.0	2.30314143773681	0.0
12	3.231798832157174E-5	0.0	0.0	2.5536381652173126	0.0
13	3.231798832157174E-5	0.0	0.0	2.6489762307659492	0.0
14	3.231798832157174E-5	0.0	0.0	2.6788380519750814	0.0
15	3.231798832157174E-5	0.0	0.0	2.7319688247757457	0.0
16	3.231798832157174E-5	0.0	0.0	2.85810593319484	0.0
17	3.231798832157174E-5	0.0	0.0	3.0178214314800473	0.0
18	3.231798832157174E-5	0.0	0.0	3.2063322573597755	0.0
19	3.231798832157174E-5	0.0	0.0	3.322709333305755	0.0
20	6.463597664314348E-5	0.0	0.0	3.448006174028489	0.0
21	6.463597664314348E-5	0.0	0.0	3.6189683322496036	0.0
22	9.695396496471522E-5	0.0	0.0	3.778231378698309	0.0
23	9.695396496471522E-5	0.0	0.0	3.958436481579393	0.0
24	9.695396496471522E-5	0.0	0.0	4.085672401601421	0.0
25	9.695396496471522E-5	0.0	0.0	4.195424289941479	0.0
26	9.695396496471522E-5	0.0	0.0	4.303915776736995	0.0
27	9.695396496471522E-5	0.0	0.0	4.399577022168847	0.0
28	1.615899416078587E-4	0.0	0.0	4.506678835466536	0.0
29	1.9390792992943044E-4	0.0	0.0	4.629260965170258	0.0
30	1.9390792992943044E-4	0.0	0.0	4.786940430191206	0.0
31	2.2622591825100218E-4	0.0	0.0	4.942907041831111	0.0
32	2.2622591825100218E-4	0.0	0.0	5.070110643864818	0.0
33	2.2622591825100218E-4	0.0	0.0	5.198897827326281	0.0
34	2.2622591825100218E-4	0.0	0.0	5.345298314423001	0.0
35	2.585439065725739E-4	0.0	0.0	5.540563599861938	0.0
36	2.9086189489414566E-4	0.0	0.0	5.694332588295976	0.0
37	2.9086189489414566E-4	0.0	0.0	5.854080404569505	0.0
38	2.9086189489414566E-4	0.0	0.0	6.008689660699904	0.0
39	3.231798832157174E-4	0.0	0.0	6.158742080476962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGAAT	55	1.8189894E-12	45.000004	34
TCACGCA	20	7.0351403E-4	45.0	17
GTCGATC	20	7.0351403E-4	45.0	6
ACGCATT	20	7.0351403E-4	45.0	17
CTATCGT	25	3.892342E-5	45.0	33
ACGTTAC	70	0.0	45.0	32
CGCATTA	35	1.2128294E-7	45.0	11
CGTTGTA	45	3.8562575E-10	45.0	36
TAGCGGA	35	1.2128294E-7	45.0	3
CGCGCGA	35	1.2128294E-7	45.0	44
AATGCGT	35	1.2128294E-7	45.0	37
TACGCAC	20	7.0351403E-4	45.0	36
CTACGCA	20	7.0351403E-4	45.0	35
TACGACC	35	1.2128294E-7	45.0	36
CGTAAGT	20	7.0351403E-4	45.0	19
ACGTAAC	50	2.1827873E-11	45.0	16
CACGTTA	70	0.0	45.0	31
CGACTCG	35	1.2128294E-7	45.0	2
CGGTAAT	70	0.0	45.0	18
TGTACGA	25	3.892342E-5	45.0	22
>>END_MODULE