FastQCFastQC Report
Thu 26 May 2016
SRR1545317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545317_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2556712
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATC302.1665492E-645.00000418
ACCCGTA351.212702E-745.00000433
ACCCACG302.1665492E-645.00000432
CGTTGTA253.8921513E-545.019
TCGAACG207.0349104E-445.01
GCGATAT2150.041.8604669
CAATTCG700.041.7857171
TTACGAG1250.041.3999981
CGGGTAT1600.040.781256
ATAGCGG5550.040.540542
CTAAGCG2000.040.51
CGCTAAT451.9299478E-840.019
ATACGGT451.9299478E-840.010
ATTTGCG2100.039.642861
CGTTTTT25600.039.638671
AACGCGC403.4604454E-739.37530
ACTCGTT403.4604454E-739.37525
TGTAGCG4000.038.81251
CGTAAGG4250.038.647062
CGTAGGT356.25129E-638.571435
Produced by FastQC (version 0.11.5)