##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545317_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2556712
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264226475254155	31.0	31.0	33.0	30.0	34.0
2	31.586493511979448	31.0	31.0	34.0	30.0	34.0
3	31.68585120263839	31.0	31.0	34.0	30.0	34.0
4	30.29441016430478	35.0	33.0	37.0	0.0	37.0
5	32.86035697411363	35.0	33.0	37.0	19.0	37.0
6	34.490413859676025	35.0	33.0	37.0	30.0	37.0
7	35.51765548876838	36.0	35.0	37.0	33.0	37.0
8	35.71117513431313	37.0	35.0	37.0	35.0	37.0
9	37.738162139497916	39.0	37.0	39.0	35.0	39.0
10	37.113256792317635	39.0	37.0	39.0	33.0	39.0
11	36.671622771747465	39.0	35.0	39.0	32.0	39.0
12	35.854788885099296	37.0	35.0	39.0	31.0	39.0
13	35.52770550613444	37.0	35.0	39.0	30.0	39.0
14	36.43775051707036	38.0	35.0	40.0	31.0	41.0
15	36.69262044375745	38.0	35.0	40.0	31.0	41.0
16	36.83740874998826	38.0	35.0	40.0	32.0	41.0
17	36.76248595852798	38.0	35.0	40.0	32.0	41.0
18	36.74462943029954	38.0	35.0	40.0	31.0	41.0
19	36.748577860940145	38.0	35.0	40.0	31.0	41.0
20	36.66376502320168	38.0	35.0	40.0	31.0	41.0
21	36.50807521535472	38.0	35.0	40.0	31.0	41.0
22	36.38130849309582	38.0	35.0	40.0	31.0	41.0
23	36.32531783008802	38.0	35.0	40.0	31.0	41.0
24	36.27737774141163	38.0	35.0	40.0	31.0	41.0
25	36.17962993094255	38.0	35.0	40.0	30.0	41.0
26	36.04598758092425	38.0	34.0	40.0	30.0	41.0
27	36.06201637102654	38.0	34.0	40.0	30.0	41.0
28	36.07015651352206	38.0	35.0	40.0	30.0	41.0
29	36.13919010041021	38.0	35.0	40.0	30.0	41.0
30	36.06543091282867	38.0	35.0	40.0	30.0	41.0
31	35.98756606141012	38.0	34.0	40.0	30.0	41.0
32	35.89828498477733	38.0	34.0	40.0	30.0	41.0
33	35.79439725710209	37.0	34.0	40.0	30.0	41.0
34	35.73327500320725	37.0	34.0	40.0	30.0	41.0
35	35.6189637315427	37.0	34.0	40.0	29.0	41.0
36	35.47012999508744	37.0	34.0	40.0	29.0	41.0
37	35.43984930645298	37.0	34.0	40.0	29.0	41.0
38	35.41230533591582	37.0	34.0	40.0	29.0	41.0
39	35.3614220138991	37.0	34.0	40.0	29.0	41.0
40	35.26744545337918	37.0	34.0	40.0	28.0	41.0
41	35.24643252740238	37.0	34.0	40.0	28.0	41.0
42	35.185061907637625	36.0	34.0	40.0	28.0	41.0
43	35.137575526692096	36.0	34.0	40.0	28.0	41.0
44	35.068215739590535	36.0	34.0	40.0	28.0	41.0
45	34.96761113492642	36.0	34.0	40.0	27.0	41.0
46	34.92097154470273	36.0	34.0	40.0	27.0	41.0
47	34.85432461692987	36.0	33.0	40.0	27.0	41.0
48	34.76023971413284	36.0	34.0	40.0	27.0	41.0
49	34.722851850345286	36.0	34.0	40.0	27.0	41.0
50	34.59464108589469	35.0	33.0	40.0	27.0	41.0
51	34.51344695843724	35.0	33.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	11.0
15	40.0
16	148.0
17	366.0
18	919.0
19	1795.0
20	3290.0
21	5154.0
22	8065.0
23	12138.0
24	17370.0
25	23760.0
26	31474.0
27	39298.0
28	48143.0
29	59473.0
30	73793.0
31	93217.0
32	120031.0
33	169551.0
34	249354.0
35	334258.0
36	196269.0
37	257234.0
38	352010.0
39	459336.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.061608816323464	25.357998867295183	30.2199074436229	12.36048487275845
2	28.123425712399364	26.648249783315446	31.845393614924166	13.382930889361022
3	29.222219788540908	26.493167787376915	31.004939156228783	13.279673267853397
4	22.191236244050952	38.92765395554916	26.326664872695872	12.55444492770402
5	23.846017854181465	32.98932378773988	29.023135965255374	14.141522392823283
6	22.46541651934203	41.90874842375676	25.227714345612647	10.398120711288561
7	91.48746515055274	3.686257975086752	3.454554130461311	1.3717227438991957
8	93.3791525991195	2.2610681218690254	2.9207435174552314	1.4390357615562488
9	89.53832109365466	4.693645588552798	3.921833980518729	1.846199337273811
10	51.93737112353679	31.917556611773247	7.950211052320324	8.194861212369638
11	42.3512699122936	21.049691948095838	22.79009133605975	13.808946803550812
12	40.030750432586856	22.815006148522006	22.178837506922953	14.975405911968185
13	21.78755370178573	38.048282325111316	22.98792355181186	17.176240421291094
14	15.820710349855597	39.85708988732403	26.36534736802581	17.956852394794566
15	15.742523991751906	26.01415411669363	42.711771994655635	15.53154989689883
16	18.84451592514135	21.83773534132902	41.25752137902118	18.060227354508445
17	19.04899730591478	23.026058468845925	26.01176823983303	31.913175985406255
18	22.868082130486343	26.685837122053634	30.409291308524388	20.03678943893563
19	29.857606175431574	27.322279552800627	23.02214719530397	19.79796707646383
20	33.06043074073263	25.864821692861767	23.61278861287466	17.46195895353094
21	25.40247004746722	28.725683612389663	26.05921198789696	19.812634352246167
22	24.951500208079754	25.64140974814527	25.22126074426842	24.185829299506555
23	22.066779519945932	30.931954791935894	24.707945204622188	22.29332048349599
24	20.85084280122282	24.867798954281906	35.881319444661735	18.40003879983354
25	19.211510721583032	27.933455156466586	32.28243932050227	20.572594801448112
26	19.452210495354972	36.377151591575426	24.801385529539502	19.369252383530096
27	20.23599060042742	33.51542919186831	27.25465363326022	18.99392657444405
28	16.70782630190651	30.33098761221444	35.81107297184822	17.150113114030834
29	18.72185838686563	26.445997828460932	33.9700756283852	20.86206815628823
30	21.00909292873034	30.58134823163501	28.18968268620009	20.21987615343457
31	28.236539743232715	28.001550428832033	23.44452562509974	20.317384202835516
32	30.16213793340822	27.80911577056782	24.410727528168994	17.618018767854966
33	26.981255612677536	29.21592263813836	24.949466345837937	18.85335540334617
34	20.34448932848127	28.625789686128122	27.550111236619536	23.479609748771075
35	21.613228240020778	26.444511544514988	29.25945511266032	22.68280510280391
36	28.275691591387687	26.84291386749857	25.316891382369235	19.56450315874451
37	21.907629799523765	31.99981069436057	27.389670795928524	18.702888710187146
38	21.08708372315693	32.53283905265826	23.92177922268914	22.45829800149567
39	21.421341160052442	31.76239639036387	26.84412636229657	19.972136087287108
40	24.508118239363682	26.267761093154018	25.653378245183657	23.57074242229864
41	18.030579901060424	25.837833905422276	27.89692386158472	28.234662331932576
42	21.96516463332593	27.042740832757072	24.507805337480328	26.484289196436674
43	22.23523807139795	28.696153497147897	25.35819443097228	23.71041400048187
44	20.924061841928225	29.92315911999474	27.431404084621185	21.721374953455843
45	18.591652090653934	34.59509714038968	23.363132022691644	23.450118746264735
46	21.27928370500862	32.35186442587198	25.553445206186698	20.815406662932702
47	22.151341253923007	26.850931978259577	27.477009534120384	23.52071723369703
48	22.817900490943053	23.32491888018674	31.17175497279318	22.685425656077022
49	20.781495921323952	25.4512436285354	31.066541714514578	22.70071873562607
50	19.101486596847828	32.24758987324345	26.311802033236436	22.339121496672288
51	18.380638883065437	33.57104750163491	24.4047432796498	23.643570335649848
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	617.5
2	1077.0
3	3283.5
4	5490.0
5	4224.0
6	2958.0
7	3101.5
8	3245.0
9	3409.5
10	3574.0
11	4045.5
12	4517.0
13	4201.5
14	3886.0
15	3816.0
16	3746.0
17	4003.5
18	4261.0
19	5360.5
20	6460.0
21	6446.5
22	6433.0
23	8110.5
24	9788.0
25	13618.0
26	22464.5
27	27481.0
28	34119.0
29	40757.0
30	48419.5
31	56082.0
32	62968.0
33	69854.0
34	76772.5
35	83691.0
36	90217.5
37	96744.0
38	109399.0
39	122054.0
40	160526.5
41	198999.0
42	227046.5
43	255094.0
44	252559.5
45	250025.0
46	229544.0
47	209063.0
48	198918.5
49	188774.0
50	178311.5
51	167849.0
52	158768.5
53	149688.0
54	136884.0
55	124080.0
56	113372.0
57	102664.0
58	100533.5
59	98403.0
60	92806.5
61	87210.0
62	73721.5
63	60233.0
64	48014.5
65	35796.0
66	28502.5
67	21209.0
68	17493.0
69	13777.0
70	11606.5
71	9436.0
72	8585.0
73	7734.0
74	5519.0
75	2770.0
76	2236.0
77	1456.0
78	676.0
79	541.5
80	407.0
81	341.5
82	276.0
83	164.0
84	52.0
85	32.0
86	12.0
87	10.0
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2556712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.18117612472706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04488019786191	12.62857952114827
2	8.670197038228348	2.8058797062331964
3	2.9939037193774443	1.4533465015116553
4	1.383987737770631	0.895781973573166
5	0.8253837192635364	0.667783966594278
6	0.5510306334566724	0.5349794238049401
7	0.38512100764952717	0.4362197597876549
8	0.2808571773977349	0.36356795626931687
9	0.22344139621718576	0.32539901271706856
>10	3.0928175309920163	12.78675388558238
>50	1.690352904403495	20.023329537466715
>100	1.851435384462731	46.093318167946634
>500	0.005009580218229583	0.5332100663036183
>1k	0.001581972700493553	0.45185052106109946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0457227877054592	0.0
2	0.0	0.0	0.0	0.1439739790793801	0.0
3	0.0	0.0	0.0	0.20045276902521678	0.0
4	0.0	0.0	0.0	0.3298768105285226	0.0
5	0.0	0.0	0.0	0.5616979933602221	0.0
6	0.0	0.0	0.0	0.7856966291080106	0.0
7	0.0	0.0	0.0	0.9096057749171592	0.0
8	0.0	0.0	0.0	1.2373704977330258	0.0
9	0.0	0.0	0.0	1.3241616576290172	0.0
10	0.0	0.0	0.0	1.4749803654068194	0.0
11	0.0	0.0	0.0	1.7351191686822762	0.0
12	0.0	0.0	0.0	1.9556367709777245	0.0
13	0.0	0.0	0.0	2.0437186511425613	0.0
14	0.0	0.0	0.0	2.0739136828864573	0.0
15	0.0	0.0	0.0	2.1221396856587678	0.0
16	0.0	0.0	0.0	2.2386956372090405	0.0
17	0.0	0.0	0.0	2.3932300548517	0.0
18	0.0	0.0	0.0	2.554257186574006	0.0
19	0.0	0.0	0.0	2.6674494428781967	0.0
20	0.0	0.0	0.0	2.782049757657491	0.0
21	0.0	0.0	0.0	2.9377575573627377	0.0
22	0.0	0.0	0.0	3.1207269336554138	0.0
23	0.0	0.0	0.0	3.3065906523691364	0.0
24	3.911273541955449E-5	0.0	0.0	3.445636426785653	0.0
25	3.911273541955449E-5	0.0	0.0	3.5642653533131616	0.0
26	3.911273541955449E-5	0.0	0.0	3.6735854488108166	0.0
27	3.911273541955449E-5	0.0	0.0	3.780637005654137	0.0
28	3.911273541955449E-5	0.0	0.0	3.8998917359483585	0.0
29	3.911273541955449E-5	0.0	0.0	4.034517771262466	0.0
30	3.911273541955449E-5	0.0	0.0	4.204853734014625	0.0
31	3.911273541955449E-5	0.0	0.0	4.366897796857839	0.0
32	3.911273541955449E-5	0.0	0.0	4.510480648583024	0.0
33	3.911273541955449E-5	0.0	0.0	4.65038690317877	0.0
34	3.911273541955449E-5	0.0	0.0	4.8004233562481815	0.0
35	3.911273541955449E-5	0.0	0.0	4.989220530118371	0.0
36	3.911273541955449E-5	0.0	0.0	5.168474196546189	0.0
37	3.911273541955449E-5	0.0	0.0	5.339670639477579	0.0
38	7.822547083910898E-5	0.0	0.0	5.5121969154132335	0.0
39	1.1733820625866348E-4	0.0	0.0	5.711632753317542	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGATC	30	2.1665492E-6	45.000004	18
ACCCGTA	35	1.212702E-7	45.000004	33
ACCCACG	30	2.1665492E-6	45.000004	32
CGTTGTA	25	3.8921513E-5	45.0	19
TCGAACG	20	7.0349104E-4	45.0	1
GCGATAT	215	0.0	41.860466	9
CAATTCG	70	0.0	41.785717	1
TTACGAG	125	0.0	41.399998	1
CGGGTAT	160	0.0	40.78125	6
ATAGCGG	555	0.0	40.54054	2
CTAAGCG	200	0.0	40.5	1
CGCTAAT	45	1.9299478E-8	40.0	19
ATACGGT	45	1.9299478E-8	40.0	10
ATTTGCG	210	0.0	39.64286	1
CGTTTTT	2560	0.0	39.63867	1
AACGCGC	40	3.4604454E-7	39.375	30
ACTCGTT	40	3.4604454E-7	39.375	25
TGTAGCG	400	0.0	38.8125	1
CGTAAGG	425	0.0	38.64706	2
CGTAGGT	35	6.25129E-6	38.57143	5
>>END_MODULE