FastQCFastQC Report
Thu 26 May 2016
SRR1545316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545316_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2165880
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25640.1183814431085748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG850.045.0000041
ACTATCG302.1663946E-645.00000412
CAACGAA302.1663946E-645.00000445
CGATTAC302.1663946E-645.00000410
CGCCTAT302.1663946E-645.00000434
CGCTAAA351.2125929E-745.00000443
TCGTCAG302.1663946E-645.00000416
TTACGCG302.1663946E-645.00000413
GCGCCTA207.034672E-445.026
AAACGTC207.034672E-445.014
TATTACG453.8562575E-1045.01
ACGTAAG750.045.01
CGCGATC253.8919537E-544.99999616
GTTCACG253.8919537E-544.9999961
CACGTTA253.8919537E-544.99999626
CCCGGTA253.8919537E-544.99999618
CGTAAGG2800.043.392862
CGGGTAT2150.042.9069756
TACGTAG950.042.631581
ACTAACG900.042.51
Produced by FastQC (version 0.11.5)