##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545316_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2165880
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21774521210778	31.0	31.0	33.0	30.0	34.0
2	31.552035662178884	31.0	31.0	34.0	30.0	34.0
3	31.65505106469426	31.0	31.0	34.0	30.0	34.0
4	30.333781649952908	35.0	33.0	37.0	0.0	37.0
5	32.858975104807286	35.0	33.0	37.0	19.0	37.0
6	34.46494265610283	35.0	33.0	37.0	30.0	37.0
7	35.41129286941105	35.0	35.0	37.0	33.0	37.0
8	35.60793488097217	37.0	35.0	37.0	35.0	37.0
9	37.66205006740909	39.0	37.0	39.0	35.0	39.0
10	37.11860029179825	39.0	37.0	39.0	33.0	39.0
11	36.60938833176353	39.0	35.0	39.0	32.0	39.0
12	35.38963700666704	37.0	35.0	39.0	31.0	39.0
13	34.86865708164811	35.0	34.0	39.0	29.0	39.0
14	35.568857923800024	37.0	34.0	40.0	27.0	41.0
15	35.974809777088296	37.0	34.0	40.0	30.0	41.0
16	36.207875782591834	37.0	35.0	40.0	31.0	41.0
17	36.1749113524295	37.0	35.0	40.0	31.0	41.0
18	36.16436644689456	36.0	35.0	40.0	31.0	41.0
19	36.11843592442795	36.0	35.0	40.0	31.0	41.0
20	35.927063364544665	36.0	34.0	40.0	30.0	41.0
21	35.74075433542024	36.0	34.0	40.0	30.0	41.0
22	35.64204111031082	36.0	34.0	40.0	30.0	41.0
23	35.579617984375865	35.0	34.0	40.0	30.0	41.0
24	35.53130967551296	35.0	34.0	40.0	30.0	41.0
25	35.39726162114245	35.0	34.0	40.0	30.0	41.0
26	35.26830249136609	35.0	34.0	40.0	29.0	41.0
27	35.269689918185676	35.0	34.0	40.0	29.0	41.0
28	35.32030260217556	36.0	34.0	40.0	29.0	41.0
29	35.37892219328864	36.0	34.0	40.0	29.0	41.0
30	35.330771787910685	36.0	34.0	40.0	29.0	41.0
31	35.17097207601529	35.0	34.0	40.0	29.0	41.0
32	35.07444779950875	35.0	34.0	40.0	29.0	41.0
33	34.92728636858921	35.0	34.0	40.0	28.0	41.0
34	34.81156712283229	35.0	34.0	40.0	27.0	41.0
35	34.69591436275324	35.0	33.0	40.0	27.0	41.0
36	34.48811984043437	35.0	33.0	40.0	26.0	41.0
37	34.40702809019891	35.0	33.0	40.0	26.0	41.0
38	34.36804255083384	35.0	33.0	40.0	27.0	41.0
39	34.30946081962066	35.0	33.0	39.0	26.0	41.0
40	34.16934548543779	35.0	33.0	39.0	25.0	41.0
41	34.118924409477906	35.0	33.0	39.0	25.0	41.0
42	34.05528145603635	35.0	33.0	39.0	25.0	41.0
43	33.96128225017083	35.0	33.0	39.0	25.0	41.0
44	33.92704766653739	35.0	33.0	39.0	26.0	41.0
45	33.84421620773081	35.0	33.0	39.0	25.0	41.0
46	33.7955006740909	35.0	33.0	38.0	25.0	41.0
47	33.70781437568102	35.0	33.0	38.0	25.0	40.0
48	33.620605943080875	35.0	33.0	38.0	24.0	40.0
49	33.58131429257392	35.0	32.0	38.0	24.0	40.0
50	33.43342890649528	35.0	32.0	38.0	24.0	40.0
51	33.329406984689825	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	12.0
14	23.0
15	79.0
16	196.0
17	493.0
18	1222.0
19	2348.0
20	4070.0
21	6437.0
22	9725.0
23	13982.0
24	19381.0
25	26215.0
26	33220.0
27	41265.0
28	49821.0
29	59715.0
30	73148.0
31	91846.0
32	115946.0
33	170619.0
34	257150.0
35	337849.0
36	161585.0
37	194893.0
38	234839.0
39	259665.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.22466618649233	25.69500618686169	31.357831458806583	12.7224961678394
2	28.04596745895433	27.046096736661312	30.94294236061093	13.964993443773432
3	28.43689401074852	26.664727501061925	31.091427041202653	13.806951446986906
4	22.649869798880825	38.6088333610357	25.681524368847768	13.059772471235709
5	23.413624023491604	32.20381553918038	29.985733281622252	14.396827155705763
6	22.184562394961862	40.892385543058715	26.064555746394074	10.85849631558535
7	90.00203150682403	4.0980109701368495	4.130699761759654	1.7692577612794798
8	92.37363104142428	2.1835004709402184	3.559153785066578	1.8837147025689327
9	89.43362513158624	3.4988087982713725	4.754557039171145	2.3130090309712448
10	63.407668014848475	18.712994256376163	8.488651264151292	9.390686464624078
11	53.45776312630433	17.56944059689364	16.363464273182263	12.609332003619775
12	47.6047149426561	19.442582229855763	18.43278482649085	14.519918000997285
13	21.9869983563263	41.39910798382182	19.545496518736034	17.068397141115852
14	15.21049180933385	40.61827063364545	25.815649989842466	18.355587567178237
15	15.165660147376586	21.529447614826307	46.48530851201359	16.819583725783517
16	17.854267087742627	16.993092876798347	43.348246440245994	21.804393595213032
17	18.919330710842704	18.453561600827378	23.833545718137664	38.79356197019225
18	25.261371821153528	22.75823221969823	29.644163111529725	22.33623284761852
19	34.588666038746375	23.537176574879496	20.11944336713022	21.75471401924391
20	36.1486785971522	22.359918370362163	21.533972334570706	19.957430697914937
21	27.118353740742794	27.873935767447872	24.782905793488098	20.224804698321236
22	27.91862891757623	23.227371784217038	21.88062127172327	26.973378026483463
23	24.044406892348608	29.796202929063476	22.205108316250207	23.95428186233771
24	23.62610116903984	22.52931833711932	36.49819011210224	17.3463903817386
25	19.20840489777827	24.823905294845513	32.78256413097679	23.18512567639943
26	18.376826047611132	34.549143996897335	24.690980109701368	22.383049845790165
27	19.390178587918076	32.62212126248915	27.361164976822355	20.62653517277042
28	16.573586717639017	26.31992538829483	38.08655142482502	19.019936469241138
29	16.700555894140027	23.645169630819808	36.713576006057586	22.940698468982585
30	21.801623358634828	29.236938334533768	27.829842835244794	21.131595471586607
31	32.032891942305206	24.361506639333665	22.38591242358764	21.219688994773485
32	34.56428795685818	23.95737529318337	21.626867601159805	19.85146914879864
33	30.739191460284044	25.34803405544167	21.546392228562986	22.366382255711304
34	20.080660055035366	25.12406042809389	26.959249819934623	27.83602969693612
35	21.744648826343102	24.787430513232497	29.197000757197998	24.2709199032264
36	33.03331671191386	23.10340369734242	22.7097992501893	21.153480340554417
37	21.95153932812529	31.223474984763698	26.1876465916856	20.637339095425418
38	23.420041738231113	32.27607254326186	21.206484200417382	23.097401518089644
39	22.599451493157517	31.58974643101187	23.453284577169555	22.357517498661053
40	28.37419432286184	24.18596598149482	21.723456516519843	25.716383179123497
41	20.345817866179104	21.232570594862135	25.45145622102794	32.97015531793082
42	23.233189281031265	23.123118547657302	22.093421611538957	31.55027055977247
43	23.418610449332373	24.93771584760005	23.300413688662346	28.34326001440523
44	20.757105656823093	28.85478419857056	25.706825862928696	24.681284281677655
45	18.609156555303155	34.90567344451216	20.25638539531276	26.228784604871926
46	22.357517498661053	32.06124069662216	22.218543963654497	23.362697841062293
47	21.626452065673075	25.027148318466395	26.72816591870279	26.618233697157738
48	24.9782536428611	21.192309823258906	30.539365061776273	23.29007147210372
49	22.34768315880843	23.047444918462702	29.921463793007923	24.683408129720945
50	20.586828448482834	31.72798123626424	24.65265850370288	23.03253181155004
51	19.846944429054243	33.09306148078379	21.940089016935378	25.11990507322659
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	670.0
2	1100.0
3	3232.0
4	5364.0
5	4007.0
6	2650.0
7	2914.0
8	3178.0
9	3490.0
10	3802.0
11	3866.5
12	3931.0
13	3952.0
14	3973.0
15	3819.5
16	3666.0
17	3587.5
18	3509.0
19	3515.5
20	3522.0
21	3766.0
22	4010.0
23	3772.5
24	3535.0
25	5060.5
26	8557.0
27	10528.0
28	13656.0
29	16784.0
30	19693.5
31	22603.0
32	25310.0
33	28017.0
34	33924.5
35	39832.0
36	41814.0
37	43796.0
38	60007.0
39	76218.0
40	112539.5
41	148861.0
42	173746.0
43	198631.0
44	203981.0
45	209331.0
46	190298.5
47	171266.0
48	162397.5
49	153529.0
50	146619.5
51	139710.0
52	131639.0
53	123568.0
54	118544.5
55	113521.0
56	112446.0
57	111371.0
58	115123.0
59	118875.0
60	114062.5
61	109250.0
62	96456.5
63	83663.0
64	71697.5
65	59732.0
66	52239.5
67	44747.0
68	38023.5
69	31300.0
70	27031.0
71	22762.0
72	19333.0
73	15904.0
74	13477.5
75	8136.5
76	5222.0
77	4386.0
78	3550.0
79	2590.5
80	1631.0
81	1256.5
82	882.0
83	747.5
84	613.0
85	328.5
86	44.0
87	28.5
88	13.0
89	8.5
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2165880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.756671972857898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45141682872399	13.313413295777297
2	8.35543615216149	2.800186055838562
3	2.7004461971557334	1.3575147331827053
4	1.3099852393629519	0.8780397178116288
5	0.8031405353637517	0.6728981249597933
6	0.5018778642126985	0.5045881644630424
7	0.3609559070492169	0.42338938117623787
8	0.2837480580629687	0.3803738505517283
9	0.21986049284516304	0.3315717142557542
>10	2.8038757137475128	11.570141789525236
>50	1.3654184866038266	16.716316778359495
>100	1.8327473158968612	49.644769123076216
>500	0.010191921612756798	1.1161653215048917
>1k	8.992872011255999E-4	0.29063194951745214
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2564	0.1183814431085748	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033843056863722826	0.0
2	0.0	0.0	0.0	0.1180582488411177	0.0
3	0.0	0.0	0.0	0.18694479841911832	0.0
4	0.0	0.0	0.0	0.357268177369014	0.0
5	0.0	0.0	0.0	0.6409865735867176	0.0
6	0.0	0.0	0.0	0.9321384379559348	0.0
7	0.0	0.0	0.0	1.0792380002585553	0.0
8	0.0	0.0	0.0	1.4258869374111216	0.0
9	0.0	0.0	0.0	1.5268620606866492	0.0
10	0.0	0.0	0.0	1.6868893936875542	0.0
11	0.0	0.0	0.0	1.9694535246643396	0.0
12	0.0	0.0	0.0	2.1761593440079783	0.0
13	0.0	0.0	0.0	2.2627292370768464	0.0
14	0.0	0.0	0.0	2.2938943985816387	0.0
15	0.0	0.0	0.0	2.3444973867434946	0.0
16	0.0	0.0	0.0	2.4553068498716457	0.0
17	0.0	0.0	0.0	2.5889707647699782	0.0
18	0.0	0.0	0.0	2.745950837534859	0.0
19	4.617060963672964E-5	0.0	0.0	2.850296415313868	0.0
20	4.617060963672964E-5	0.0	0.0	2.9519179271243097	0.0
21	4.617060963672964E-5	0.0	0.0	3.1046041331929746	0.0
22	4.617060963672964E-5	0.0	0.0	3.2525809370786933	0.0
23	4.617060963672964E-5	0.0	0.0	3.4111769811808594	0.0
24	4.617060963672964E-5	0.0	0.0	3.5361608214674867	0.0
25	4.617060963672964E-5	0.0	0.0	3.6406449110754058	0.0
26	4.617060963672964E-5	0.0	0.0	3.7390806508209136	0.0
27	4.617060963672964E-5	0.0	0.0	3.8437494228673796	0.0
28	4.617060963672964E-5	0.0	0.0	3.943247086634532	0.0
29	4.617060963672964E-5	0.0	0.0	4.0663379319260535	0.0
30	4.617060963672964E-5	0.0	0.0	4.2258573882209545	0.0
31	9.234121927345928E-5	0.0	0.0	4.370648420041738	0.0
32	9.234121927345928E-5	0.0	0.0	4.493508412285076	0.0
33	9.234121927345928E-5	0.0	0.0	4.618169058304246	0.0
34	1.3851182891018893E-4	0.0	0.0	4.765037767558683	0.0
35	1.3851182891018893E-4	0.0	0.0	4.952167248416348	0.0
36	1.3851182891018893E-4	0.0	0.0	5.098666592793691	0.0
37	1.8468243854691857E-4	0.0	0.0	5.248444050455242	0.0
38	1.8468243854691857E-4	0.0	0.0	5.4277245276746635	0.0
39	1.8468243854691857E-4	0.0	0.0	5.737760171385303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	85	0.0	45.000004	1
ACTATCG	30	2.1663946E-6	45.000004	12
CAACGAA	30	2.1663946E-6	45.000004	45
CGATTAC	30	2.1663946E-6	45.000004	10
CGCCTAT	30	2.1663946E-6	45.000004	34
CGCTAAA	35	1.2125929E-7	45.000004	43
TCGTCAG	30	2.1663946E-6	45.000004	16
TTACGCG	30	2.1663946E-6	45.000004	13
GCGCCTA	20	7.034672E-4	45.0	26
AAACGTC	20	7.034672E-4	45.0	14
TATTACG	45	3.8562575E-10	45.0	1
ACGTAAG	75	0.0	45.0	1
CGCGATC	25	3.8919537E-5	44.999996	16
GTTCACG	25	3.8919537E-5	44.999996	1
CACGTTA	25	3.8919537E-5	44.999996	26
CCCGGTA	25	3.8919537E-5	44.999996	18
CGTAAGG	280	0.0	43.39286	2
CGGGTAT	215	0.0	42.906975	6
TACGTAG	95	0.0	42.63158	1
ACTAACG	90	0.0	42.5	1
>>END_MODULE