FastQCFastQC Report
Thu 26 May 2016
SRR1545315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545315_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4361256
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG53970.12374875494582296No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50600.11602162312875006No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT50580.11597576477968732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1000.045.01
TCTAGCG1750.045.01
ATCGTAC207.0354587E-445.027
TCGAATA253.8926053E-545.041
ATTGGGC10900.042.5229384
TATTACG750.042.01
CGTTTTT41650.040.948381
CGTATTT7600.040.8552671
ACTATCG501.0822987E-940.51
ACTCGAT501.0822987E-940.513
AATCGAC501.0822987E-940.535
CGAATAT6850.040.0729914
CGTATCA451.9304935E-840.00000422
CGCATTA900.040.00000412
ATCGATA451.9304935E-840.00000436
CAACCCG850.039.70588323
CGTATCT403.4611912E-739.37533
GCGAAGT403.4611912E-739.37537
TATATCG403.4611912E-739.37512
ATCCGTT403.4611912E-739.37517