FastQCFastQC Report
Thu 26 May 2016
SRR1545314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545314_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1854110
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27530.14848094233891193No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23210.12518135385710666No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.10662797784381725No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.2124656E-745.01
GTACCGA351.2124656E-745.020
CGTATAT207.034407E-445.014
GTCGAAC207.034407E-445.027
CGCCTAT207.034407E-445.044
TATTACG351.2124656E-745.01
GACGATT207.034407E-445.024
TATACGT207.034407E-445.011
GCTCGAT207.034407E-445.043
TCGACTA406.8193913E-945.035
TAATGCG453.8562575E-1045.01
TGCGTTA351.2124656E-745.021
TAGTACG406.8193913E-945.01
AGTCGAC207.034407E-445.018
AAGTCGA207.034407E-445.025
TACCCGA302.1662254E-644.99999640
CGGTCTA650.044.99999631
GCTACGC302.1662254E-644.99999641
TATAGCG1600.043.593751
CGTATTT3800.043.2236821