##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545314_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1854110
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31751406335115	31.0	31.0	33.0	30.0	34.0
2	31.64056016093975	31.0	31.0	34.0	30.0	34.0
3	31.74670380937485	31.0	31.0	34.0	30.0	34.0
4	30.320602876851968	35.0	33.0	37.0	0.0	37.0
5	32.905428480510864	35.0	33.0	37.0	19.0	37.0
6	34.55915344828516	35.0	33.0	37.0	30.0	37.0
7	35.513824422499205	36.0	35.0	37.0	33.0	37.0
8	35.6794774851546	37.0	35.0	37.0	35.0	37.0
9	37.703292684900035	39.0	37.0	39.0	35.0	39.0
10	37.19977293688077	39.0	37.0	39.0	34.0	39.0
11	36.75382690347391	39.0	35.0	39.0	32.0	39.0
12	35.725252547044136	37.0	35.0	39.0	31.0	39.0
13	35.3130164876949	37.0	35.0	39.0	30.0	39.0
14	36.173708140293726	38.0	35.0	40.0	31.0	41.0
15	36.483843461283314	38.0	35.0	40.0	31.0	41.0
16	36.66754345750792	38.0	35.0	40.0	32.0	41.0
17	36.59185970627417	38.0	35.0	40.0	32.0	41.0
18	36.58667716586395	38.0	35.0	40.0	31.0	41.0
19	36.56464341382119	38.0	35.0	40.0	31.0	41.0
20	36.45466342342148	38.0	35.0	40.0	31.0	41.0
21	36.26756988528189	37.0	35.0	40.0	31.0	41.0
22	36.13540458764582	37.0	35.0	40.0	31.0	41.0
23	36.07836590062078	37.0	34.0	40.0	31.0	41.0
24	36.01233260162558	37.0	34.0	40.0	30.0	41.0
25	35.89816839346101	37.0	34.0	40.0	30.0	41.0
26	35.722311513340635	36.0	34.0	40.0	30.0	41.0
27	35.73036227624035	36.0	34.0	40.0	30.0	41.0
28	35.73148680499	36.0	34.0	40.0	30.0	41.0
29	35.78851686253782	36.0	34.0	40.0	30.0	41.0
30	35.76528900658537	36.0	34.0	40.0	30.0	41.0
31	35.68006590763223	36.0	34.0	40.0	30.0	41.0
32	35.56864910927615	36.0	34.0	40.0	30.0	41.0
33	35.43011957219367	36.0	34.0	40.0	29.0	41.0
34	35.33053810183862	36.0	34.0	40.0	29.0	41.0
35	35.23853061576713	36.0	34.0	40.0	29.0	41.0
36	35.05316081570133	36.0	34.0	40.0	27.0	41.0
37	34.99084520335902	36.0	34.0	40.0	27.0	41.0
38	34.97893814282863	35.0	34.0	40.0	28.0	41.0
39	34.90276844415919	35.0	34.0	40.0	27.0	41.0
40	34.7333146361327	35.0	33.0	40.0	27.0	41.0
41	34.703327742151224	35.0	34.0	40.0	27.0	41.0
42	34.65987131292103	35.0	33.0	40.0	27.0	41.0
43	34.6223066592597	35.0	33.0	40.0	27.0	41.0
44	34.5330363354925	35.0	33.0	40.0	27.0	41.0
45	34.443095609214126	35.0	33.0	39.0	27.0	41.0
46	34.38051517978977	35.0	33.0	39.0	27.0	41.0
47	34.30400407742798	35.0	33.0	39.0	27.0	41.0
48	34.229582926579326	35.0	33.0	39.0	26.0	41.0
49	34.1868049899952	35.0	33.0	39.0	26.0	41.0
50	34.02352503357406	35.0	33.0	39.0	26.0	40.0
51	33.9276278106477	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	10.0
14	11.0
15	29.0
16	111.0
17	289.0
18	691.0
19	1428.0
20	2582.0
21	4134.0
22	6396.0
23	9328.0
24	13332.0
25	18339.0
26	24657.0
27	31005.0
28	37009.0
29	44973.0
30	56196.0
31	71044.0
32	91055.0
33	134002.0
34	203650.0
35	275400.0
36	143667.0
37	180895.0
38	228170.0
39	275524.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.038174649831994	26.246123477032107	29.445502154672592	12.270199718463305
2	28.269358344434796	27.03259245675823	31.288596685202062	13.409452513604911
3	29.697375020899514	25.793669199777792	30.09735128983717	14.411604489485521
4	23.23939787822729	39.33051437077628	24.65668164240525	12.773406108591184
5	24.36317154861362	33.029485844960654	28.314501297118294	14.29284130930743
6	22.782628862365232	41.93893566185394	24.502052197550306	10.776383278230526
7	90.55012917248708	3.996958109281542	3.776043492565166	1.6768692256662225
8	91.81645101962667	2.941950585456095	3.459988889548085	1.7816095053691527
9	87.89785935030824	4.729223185247909	4.827221685876242	2.5456957785676146
10	59.25743348560765	21.663169930586644	9.15851810302517	9.920878480780535
11	50.76753806408465	20.498675914589747	15.063615427347893	13.670170593977705
12	47.5325627929303	20.729244758940947	18.098332892870435	13.639859555258319
13	23.78585952289778	39.18386719234566	18.778119960520144	18.252153324236424
14	15.444552912178889	41.96040148642745	23.649513782893138	18.945531818500523
15	15.755807368494857	24.07963928785239	44.69907394922632	15.465479394426437
16	18.579965589959606	17.87973744815572	44.639800227602464	18.900496734282214
17	18.222489496308203	19.636591140762956	25.453829600185536	36.68708976274331
18	23.465382312807762	26.081731936077148	27.362238486389696	23.090647264725394
19	33.57050013213887	25.609915269320588	20.325223422558533	20.494361175982007
20	38.338825636019436	22.938390926104706	20.531683664938974	18.19109977293688
21	26.992573256171426	27.501334872256773	23.048740365997702	22.457351505574103
22	25.09139155713523	26.45625124722913	21.022161576174014	27.430195619461628
23	25.479124755273418	30.400677413961414	20.87621554276715	23.243982287998016
24	24.019664421204784	22.95910167142187	33.197383111034405	19.82385079633894
25	21.065902238809993	24.824471040013808	30.87891225439699	23.230714466779208
26	20.60454881317721	36.306637686005686	22.3020209156954	20.786792585121702
27	23.150298525977423	33.18638052758466	24.717789127937394	18.945531818500523
28	18.829519284184865	30.28202210224852	33.591965956712386	17.29649265685423
29	19.894127101412536	26.117490332288806	31.950100048001467	22.03828251829719
30	21.85382744281623	29.785665359660427	26.64485925862004	21.715647938903302
31	31.127818737831088	27.43785428049037	19.604014864274504	21.830312117404038
32	34.149214447902224	27.15696479712638	20.990070707778933	17.703750047192454
33	30.500617546963234	27.59739174051162	21.21929119631521	20.682699516209937
34	21.73107312942598	28.01667646471892	24.844372771842018	25.407877634013083
35	24.277739724180332	23.854733537923856	26.29854755111617	25.56897918677964
36	30.737604564993447	26.30690735716867	21.890826326377617	21.06466175146027
37	24.508092831601143	32.49677742960234	24.62383569475382	18.371294044042692
38	23.46991278834589	32.074256651439235	20.570084838547874	23.885745721667
39	24.87085448004703	29.713231685282963	23.851605352433243	21.56430848223676
40	26.316399782105698	24.92683821348248	23.870320531144323	24.8864414732675
41	18.225294076403234	24.635377620529525	26.12730636262142	31.012021940445823
42	23.841034242844277	26.619833774695135	20.981549099028644	28.55758288343194
43	24.104017560986133	27.893059203607123	22.301805178765015	25.701118056641732
44	22.827502143885745	30.524672214701393	24.49579582656908	22.15202981484378
45	20.416749815275253	33.252449962515705	20.41124852355038	25.919551698658655
46	22.009859177718692	33.47244769727794	21.814023979159813	22.70366914584356
47	24.089401383952406	26.073695735420227	25.08400256726947	24.75290031335789
48	25.528798183495045	21.07480138718846	28.448743602051657	24.947656827264833
49	20.605519629363954	25.771933704041295	28.481859220866074	25.140687445728677
50	19.75594759749961	32.671686145913675	23.91125661368527	23.661109642901444
51	18.80519494528372	33.9776496540119	21.413076894035413	25.804078506668972
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	308.0
1	747.0
2	1186.0
3	2844.5
4	4503.0
5	3480.5
6	2458.0
7	2557.0
8	2656.0
9	2873.5
10	3091.0
11	3043.0
12	2995.0
13	2898.0
14	2801.0
15	2764.5
16	2728.0
17	2705.0
18	2682.0
19	2897.5
20	3113.0
21	2873.5
22	2634.0
23	3225.0
24	3816.0
25	5069.0
26	8615.0
27	10908.0
28	14330.0
29	17752.0
30	20639.0
31	23526.0
32	27412.5
33	31299.0
34	33664.0
35	36029.0
36	37065.5
37	38102.0
38	50388.5
39	62675.0
40	93320.0
41	123965.0
42	147272.5
43	170580.0
44	170719.5
45	170859.0
46	159725.0
47	148591.0
48	134944.0
49	121297.0
50	118533.5
51	115770.0
52	111730.0
53	107690.0
54	99533.0
55	91376.0
56	92978.5
57	94581.0
58	103046.0
59	111511.0
60	108673.5
61	105836.0
62	91520.5
63	77205.0
64	63523.0
65	49841.0
66	44129.0
67	38417.0
68	30699.5
69	22982.0
70	19302.5
71	15623.0
72	13850.5
73	12078.0
74	9377.0
75	5129.5
76	3583.0
77	2957.0
78	2331.0
79	1597.0
80	863.0
81	630.0
82	397.0
83	376.0
84	355.0
85	210.0
86	65.0
87	42.5
88	20.0
89	19.5
90	19.0
91	16.5
92	14.0
93	7.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1854110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.048491326488953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76689665240474	12.640898577405693
2	8.017119014114884	2.573253299229048
3	2.589172417020046	1.2465693323199194
4	1.2068681245726516	0.7747365051768076
5	0.7041104547576098	0.5649955263033847
6	0.4715265473605329	0.45403738233148777
7	0.33183551693963115	0.37278215907986545
8	0.26576204378502477	0.3412063883675159
9	0.2199556029218069	0.317696002713294
>10	3.6752982103613068	15.467712372625636
>50	2.0225811914622347	23.675101957019617
>100	1.7200740161589319	40.23473870839063
>500	0.006233480766297551	0.6038762153910009
>1k	0.002566727374357815	0.7323955736461115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2753	0.14848094233891193	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2321	0.12518135385710666	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1977	0.10662797784381725	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04816326970891695	0.0
2	0.0	0.0	0.0	0.14368079563779926	0.0
3	0.0	0.0	0.0	0.20538155772850586	0.0
4	0.0	0.0	0.0	0.35979526565306263	0.0
5	0.0	0.0	0.0	0.6068679851788729	0.0
6	0.0	0.0	0.0	0.8675321313190695	0.0
7	0.0	0.0	0.0	1.0067903198839336	0.0
8	0.0	0.0	0.0	1.3406432196579492	0.0
9	0.0	0.0	0.0	1.4341651789807508	0.0
10	0.0	0.0	0.0	1.5843720167627595	0.0
11	0.0	0.0	0.0	1.8366224226178598	0.0
12	0.0	0.0	0.0	2.0315946734551886	0.0
13	0.0	0.0	0.0	2.1183209194707975	0.0
14	0.0	0.0	0.0	2.15235342023936	0.0
15	0.0	0.0	0.0	2.211141733769841	0.0
16	0.0	0.0	0.0	2.3328173625081576	0.0
17	0.0	0.0	0.0	2.476174552750376	0.0
18	0.0	0.0	0.0	2.6322062876528363	0.0
19	0.0	0.0	0.0	2.728964300931444	0.0
20	0.0	0.0	0.0	2.8263695250012133	0.0
21	0.0	0.0	0.0	2.974472927711948	0.0
22	0.0	0.0	0.0	3.134010387733198	0.0
23	0.0	0.0	0.0	3.2968378359428514	0.0
24	0.0	0.0	0.0	3.4191067412397325	0.0
25	0.0	0.0	0.0	3.526543732572501	0.0
26	0.0	0.0	0.0	3.629719919530125	0.0
27	0.0	0.0	0.0	3.7205451672230883	0.0
28	0.0	0.0	0.0	3.82442249920447	0.0
29	0.0	0.0	0.0	3.930241463559336	0.0
30	0.0	0.0	0.0	4.068259164774474	0.0
31	0.0	0.0	0.0	4.215122080135483	0.0
32	0.0	0.0	0.0	4.34947225353404	0.0
33	0.0	0.0	0.0	4.468289367944728	0.0
34	0.0	0.0	0.0	4.596329236129463	0.0
35	0.0	0.0	0.0	4.755327353824746	0.0
36	0.0	0.0	0.0	4.91664464352169	0.0
37	0.0	0.0	0.0	5.0767753801015045	0.0
38	0.0	0.0	0.0	5.213714396664707	0.0
39	5.3934232596771497E-5	0.0	0.0	5.365215656029038	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2124656E-7	45.0	1
GTACCGA	35	1.2124656E-7	45.0	20
CGTATAT	20	7.034407E-4	45.0	14
GTCGAAC	20	7.034407E-4	45.0	27
CGCCTAT	20	7.034407E-4	45.0	44
TATTACG	35	1.2124656E-7	45.0	1
GACGATT	20	7.034407E-4	45.0	24
TATACGT	20	7.034407E-4	45.0	11
GCTCGAT	20	7.034407E-4	45.0	43
TCGACTA	40	6.8193913E-9	45.0	35
TAATGCG	45	3.8562575E-10	45.0	1
TGCGTTA	35	1.2124656E-7	45.0	21
TAGTACG	40	6.8193913E-9	45.0	1
AGTCGAC	20	7.034407E-4	45.0	18
AAGTCGA	20	7.034407E-4	45.0	25
TACCCGA	30	2.1662254E-6	44.999996	40
CGGTCTA	65	0.0	44.999996	31
GCTACGC	30	2.1662254E-6	44.999996	41
TATAGCG	160	0.0	43.59375	1
CGTATTT	380	0.0	43.223682	1
>>END_MODULE