FastQCFastQC Report
Thu 26 May 2016
SRR1545313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545313_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3527795
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51310.14544495924508086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA406.8212103E-945.00000445
CGTTGAA207.0352736E-445.00000418
TGACGTA406.8212103E-945.00000418
CCCGTTA207.0352736E-445.00000432
TCGTAGA207.0352736E-445.00000429
TATACGA207.0352736E-445.00000443
TAAGCTT406.8212103E-945.0000041
CTATGCG453.8562575E-1045.01
TACCCGT253.8924532E-545.044
TCCGCGT253.8924532E-545.013
GTCGATA253.8924532E-545.021
TAGACGT351.212884E-745.037
TAGCGGA351.212884E-745.044
CCGTGTA253.8924532E-545.022
ACCGAGT351.212884E-745.040
TCGGATT900.045.024
CCGTAGA253.8924532E-545.041
CTTATCG502.1827873E-1145.010
TACGATC351.212884E-745.011
TATTCGA351.212884E-745.044