##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545313_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3527795
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312312932015608	31.0	31.0	33.0	30.0	34.0
2	31.625985920383695	31.0	31.0	34.0	30.0	34.0
3	31.720169397598216	31.0	31.0	34.0	30.0	34.0
4	30.29234776964081	35.0	33.0	37.0	0.0	37.0
5	32.8760713136676	35.0	33.0	37.0	19.0	37.0
6	34.517389474161625	35.0	33.0	37.0	30.0	37.0
7	35.522514488511945	36.0	35.0	37.0	33.0	37.0
8	35.71169328149737	37.0	35.0	37.0	35.0	37.0
9	37.756169221851046	39.0	37.0	39.0	35.0	39.0
10	37.218988631709045	39.0	37.0	39.0	34.0	39.0
11	36.73409339261493	39.0	35.0	39.0	32.0	39.0
12	35.34533299128776	35.0	35.0	39.0	31.0	39.0
13	34.820472278009355	35.0	34.0	39.0	30.0	39.0
14	35.5506303512534	36.0	34.0	40.0	29.0	41.0
15	35.958044330807205	36.0	34.0	40.0	31.0	41.0
16	36.20256732604928	36.0	35.0	40.0	32.0	41.0
17	36.1499463545926	36.0	35.0	40.0	31.0	41.0
18	36.146520135098555	36.0	35.0	40.0	31.0	41.0
19	36.122509953101016	36.0	35.0	40.0	31.0	41.0
20	36.00688957266508	36.0	34.0	40.0	31.0	41.0
21	35.82178783064209	35.0	34.0	40.0	31.0	41.0
22	35.67868342689981	35.0	34.0	40.0	30.0	41.0
23	35.665830639252	35.0	34.0	40.0	30.0	41.0
24	35.613647051486836	35.0	34.0	40.0	30.0	41.0
25	35.49334272541347	35.0	34.0	40.0	30.0	41.0
26	35.341344947764824	35.0	34.0	40.0	30.0	41.0
27	35.32644980788283	35.0	34.0	40.0	30.0	41.0
28	35.38553940917769	36.0	34.0	40.0	30.0	41.0
29	35.47677571967759	36.0	34.0	40.0	30.0	41.0
30	35.44974296975873	36.0	34.0	40.0	30.0	41.0
31	35.289640129315906	35.0	34.0	40.0	30.0	41.0
32	35.15987748721227	35.0	34.0	40.0	29.0	41.0
33	35.02473159579851	35.0	34.0	40.0	29.0	41.0
34	34.95578144421657	35.0	34.0	40.0	29.0	41.0
35	34.810074564990316	35.0	34.0	40.0	29.0	41.0
36	34.59266255550563	35.0	33.0	40.0	27.0	41.0
37	34.5318775609127	35.0	33.0	40.0	27.0	41.0
38	34.549040973185804	35.0	33.0	40.0	27.0	41.0
39	34.52128170712867	35.0	33.0	39.0	27.0	41.0
40	34.4094628514412	35.0	33.0	39.0	27.0	41.0
41	34.43759572197364	35.0	33.0	39.0	27.0	41.0
42	34.36763360682806	35.0	33.0	39.0	27.0	41.0
43	34.26738033247397	35.0	33.0	39.0	27.0	41.0
44	34.19424371314093	35.0	33.0	39.0	27.0	41.0
45	34.108346431694585	35.0	33.0	39.0	27.0	41.0
46	34.07524416809934	35.0	33.0	39.0	27.0	41.0
47	34.00656585770999	35.0	33.0	39.0	26.0	41.0
48	33.95304205601516	35.0	33.0	38.0	26.0	41.0
49	33.93441937527549	35.0	33.0	38.0	26.0	40.0
50	33.73978901835282	35.0	33.0	38.0	26.0	40.0
51	33.628166602651234	35.0	33.0	38.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	5.0
13	4.0
14	14.0
15	69.0
16	199.0
17	543.0
18	1450.0
19	2924.0
20	5317.0
21	8763.0
22	13422.0
23	19725.0
24	27941.0
25	38316.0
26	50175.0
27	62045.0
28	75619.0
29	91791.0
30	113671.0
31	144688.0
32	187816.0
33	282236.0
34	436549.0
35	605808.0
36	235352.0
37	288260.0
38	375438.0
39	459364.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.86086493121057	25.202173028761592	30.691154106176803	12.245807933851031
2	27.34373737703013	26.354025673260495	32.91855110628594	13.383685843423443
3	28.362844212886518	25.86941701544449	31.37736177980863	14.390376991860354
4	22.635187135306897	38.196153688068605	25.758101023443825	13.410558153180668
5	23.73927623345461	32.78586199028005	29.34161423778876	14.133247538476585
6	21.808750224998903	40.732837367250646	25.91729394706892	11.541118460681531
7	90.03357621403738	4.341635497527492	3.993599401325757	1.63118888710937
8	92.7896887432518	2.307560388287868	3.1001801408528555	1.8025707276074714
9	89.51710629444172	3.9138895542399714	4.472453756524968	2.0965503947933484
10	64.74293432583242	18.055640988209348	7.76314950273471	9.438275183223515
11	57.4463935687873	16.171092708051347	14.689090494203885	11.693423228957466
12	48.546046468119606	21.383555450359218	17.48715557451609	12.583242507005085
13	20.133029271825603	47.148544629152205	19.14935533385585	13.569070765166344
14	13.23571806184883	47.369844336193005	25.821596776456683	13.572840825501482
15	11.990917839613696	21.429079637563973	52.83648851478048	13.743514008041851
16	14.791250625390647	15.48233386577168	49.761621636177836	19.964793872659833
17	15.82107236956796	16.55507193586929	26.180574551525808	41.44328114303694
18	23.13833428529719	22.276152667601153	33.146398812856184	21.439114234245473
19	33.87705351359702	22.94339665428405	21.361331936804717	21.818217895314213
20	35.50537942255715	23.753874587383905	21.054738157971197	19.68600783208775
21	23.883615686285626	29.155889160226145	24.764335796155954	22.19615935733227
22	23.75523521066275	25.36887772673866	22.469843060608678	28.406044001989912
23	20.635581149131397	31.98499345908705	22.576056715313673	24.80336867646788
24	20.80738818440414	22.838118428083266	38.81764671700028	17.53684667051232
25	17.021765720513805	25.288856070151468	35.772628511577345	21.916749697757382
26	16.959035318095296	38.21321817169082	24.020981944812554	20.806764565401334
27	18.254150255329463	35.55739491665474	27.358279038322802	18.830175789692998
28	14.748419338425279	27.947145454880456	40.149385097490075	17.155050109204193
29	14.861436109524503	24.96942140912383	38.18345453746604	21.98568794388563
30	19.838624409865087	30.43875848795069	29.87211558494754	19.850501517236687
31	33.3007728623687	25.29826704783016	22.138332867981276	19.262627221819862
32	35.69552652577602	24.75761771871665	22.463096636851066	17.08375911865627
33	30.893178316767273	26.1963634508241	22.165488640921595	20.744969591487035
34	19.503372503220852	26.451622047199454	27.133776197313054	26.911229252266644
35	21.322185671219557	24.50791500072992	31.202266571612014	22.967632756438512
36	34.429041370034255	23.13549965346626	24.148682108796006	18.28677686770348
37	21.648650219187907	32.90775115901009	26.94790938815889	18.495689233643112
38	20.38412095940949	35.07505396430348	21.6188015460082	22.922023530278828
39	22.055930120656104	32.869058434517875	24.702427436968417	20.3725840078576
40	25.58136172878526	24.99538663669516	22.82641139862152	26.59684023589806
41	17.387574958295478	22.423496830172954	25.94702923497539	34.24189897655618
42	23.040397755538518	24.35002600774705	21.82726037085488	30.782315865859555
43	23.134139030187413	25.126119856737706	24.345632328409106	27.39410878466578
44	19.895203661210473	31.28761733604135	27.164276835813872	21.652902166934304
45	17.2159663472509	38.66636241618348	20.68867380332474	23.428997433240877
46	23.210022124301442	32.456251000979364	23.94070517136058	20.393021703358613
47	21.537816114598495	25.03949350798445	27.14783597119447	26.274854406222587
48	24.044707813237448	20.494586561860878	32.16859256277647	23.292113062125207
49	21.419923776750068	21.563724649533206	32.80802881119793	24.208322762518797
50	19.247943828935636	33.53154023972482	25.085272812053987	22.13524311928556
51	17.670471215022417	34.708932917020405	23.102277768407745	24.518318099549436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	660.0
1	1060.5
2	1461.0
3	4096.5
4	6732.0
5	5253.5
6	3775.0
7	4317.0
8	4859.0
9	5515.5
10	6172.0
11	6326.5
12	6481.0
13	6578.5
14	6676.0
15	6369.0
16	6062.0
17	5842.0
18	5622.0
19	5328.5
20	5035.0
21	5551.5
22	6068.0
23	7731.0
24	9394.0
25	11410.0
26	14205.5
27	14985.0
28	20107.0
29	25229.0
30	29458.5
31	33688.0
32	40793.5
33	47899.0
34	56162.0
35	64425.0
36	69715.5
37	75006.0
38	105626.5
39	136247.0
40	216853.5
41	297460.0
42	356886.0
43	416312.0
44	403655.0
45	390998.0
46	356908.0
47	322818.0
48	307822.5
49	292827.0
50	273583.5
51	254340.0
52	236913.0
53	219486.0
54	196563.5
55	173641.0
56	167045.0
57	160449.0
58	151757.0
59	143065.0
60	133767.0
61	124469.0
62	106924.0
63	89379.0
64	73439.0
65	57499.0
66	48764.5
67	40030.0
68	32182.0
69	24334.0
70	21125.0
71	17916.0
72	15260.0
73	12604.0
74	8697.0
75	3963.5
76	3137.0
77	2265.0
78	1393.0
79	987.5
80	582.0
81	406.0
82	230.0
83	151.5
84	73.0
85	55.0
86	37.0
87	27.5
88	18.0
89	11.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3527795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.365680612437652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31513858966149	10.333694488957688
2	10.19011610843254	2.7239567461793084
3	3.7435342090352766	1.5010464779909976
4	1.7812121410339035	0.9522845032022162
5	1.038020544390387	0.6936925532735287
6	0.6338515032098616	0.5083114048569904
7	0.4329071294832637	0.40502688992643454
8	0.3000986902382136	0.32088185967478594
9	0.2183666623147412	0.2626757158412571
>10	1.665155793933645	4.9566314753421565
>50	0.6295322512649904	6.166265798969957
>100	1.963461273007499	62.845822170138845
>500	0.08095073637175151	6.824481095524304
>1k	0.00742241708852736	1.346158750794325
>5k	2.3195053401648E-4	0.15907006932720005
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5131	0.14544495924508086	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07287838437324164	0.0
2	0.0	0.0	0.0	0.24817201679802822	0.0
3	0.0	0.0	0.0	0.3539604767283813	0.0
4	0.0	0.0	0.0	0.581751490662014	0.0
5	0.0	0.0	0.0	0.9276899593088601	0.0
6	0.0	0.0	0.0	1.2974676816538375	0.0
7	0.0	0.0	0.0	1.4777786124193724	0.0
8	0.0	0.0	0.0	1.906204867346317	0.0
9	0.0	0.0	0.0	2.028264113986215	0.0
10	2.834631830931219E-5	0.0	0.0	2.235192237644194	0.0
11	2.834631830931219E-5	0.0	0.0	2.553804855440863	0.0
12	2.834631830931219E-5	0.0	0.0	2.8080146380387747	0.0
13	5.669263661862438E-5	0.0	0.0	2.910798388228341	0.0
14	5.669263661862438E-5	0.0	0.0	2.954083216286661	0.0
15	5.669263661862438E-5	0.0	0.0	3.021660839136061	0.0
16	5.669263661862438E-5	0.0	0.0	3.1745041874598723	0.0
17	5.669263661862438E-5	0.0	0.0	3.355722200411305	0.0
18	5.669263661862438E-5	0.0	0.0	3.5766534053140844	0.0
19	5.669263661862438E-5	0.0	0.0	3.704920495663722	0.0
20	5.669263661862438E-5	0.0	0.0	3.8454615418412916	0.0
21	5.669263661862438E-5	0.0	0.0	4.007630828888867	0.0
22	5.669263661862438E-5	0.0	0.0	4.16741902519846	0.0
23	5.669263661862438E-5	0.0	0.0	4.3393394457444385	0.0
24	5.669263661862438E-5	0.0	0.0	4.4663026054518475	0.0
25	5.669263661862438E-5	0.0	0.0	4.580198112418664	0.0
26	5.669263661862438E-5	0.0	0.0	4.688027507267288	0.0
27	5.669263661862438E-5	0.0	0.0	4.789195517313223	0.0
28	5.669263661862438E-5	0.0	0.0	4.908051629984168	0.0
29	5.669263661862438E-5	0.0	0.0	5.025490426739649	0.0
30	5.669263661862438E-5	0.0	0.0	5.178673930883172	0.0
31	5.669263661862438E-5	0.0	0.0	5.332055859254861	0.0
32	5.669263661862438E-5	0.0	0.0	5.450458430832858	0.0
33	5.669263661862438E-5	0.0	0.0	5.581531806695117	0.0
34	5.669263661862438E-5	0.0	0.0	5.724992523658546	0.0
35	5.669263661862438E-5	0.0	0.0	5.905275108105771	0.0
36	5.669263661862438E-5	0.0	0.0	6.056871218423973	0.0
37	5.669263661862438E-5	0.0	0.0	6.198007537286038	0.0
38	8.503895492793658E-5	0.0	0.0	6.342743838573386	0.0
39	1.1338527323724876E-4	0.0	0.0	6.490088001145192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	40	6.8212103E-9	45.000004	45
CGTTGAA	20	7.0352736E-4	45.000004	18
TGACGTA	40	6.8212103E-9	45.000004	18
CCCGTTA	20	7.0352736E-4	45.000004	32
TCGTAGA	20	7.0352736E-4	45.000004	29
TATACGA	20	7.0352736E-4	45.000004	43
TAAGCTT	40	6.8212103E-9	45.000004	1
CTATGCG	45	3.8562575E-10	45.0	1
TACCCGT	25	3.8924532E-5	45.0	44
TCCGCGT	25	3.8924532E-5	45.0	13
GTCGATA	25	3.8924532E-5	45.0	21
TAGACGT	35	1.212884E-7	45.0	37
TAGCGGA	35	1.212884E-7	45.0	44
CCGTGTA	25	3.8924532E-5	45.0	22
ACCGAGT	35	1.212884E-7	45.0	40
TCGGATT	90	0.0	45.0	24
CCGTAGA	25	3.8924532E-5	45.0	41
CTTATCG	50	2.1827873E-11	45.0	10
TACGATC	35	1.212884E-7	45.0	11
TATTCGA	35	1.212884E-7	45.0	44
>>END_MODULE