FastQCFastQC Report
Thu 26 May 2016
SRR1545312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545312_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2978412
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39460.13248670768181164No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.12449587229704957No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33090.11109947179906608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG1050.045.0000042
CGGTATG207.035096E-445.00000424
CACGATA351.212793E-745.013
AACGCAC351.212793E-745.040
TTACGAG551.8189894E-1245.01
TAAATCG351.212793E-745.01
CGCGTAA351.212793E-745.031
TCGTAAG1550.043.5483861
GTCGATG1050.042.8571471
GTTTACG1500.042.01
TTAAACG603.6379788E-1241.2499961
TATCGTG603.6379788E-1241.2499961
CGTAACG556.184564E-1140.90909241
TCGGTAC1500.040.536
TCTCAAC6700.040.29850835
CGGGTAT3300.040.2272726
TTAGTAG5000.040.051
ACGCATT451.9301297E-840.09
CTATACG451.9301297E-840.01
TATCGTT451.9301297E-840.023