##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545312_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2978412
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373828066768464	31.0	31.0	33.0	30.0	34.0
2	31.693109952551897	31.0	31.0	34.0	30.0	34.0
3	31.7835376704096	31.0	31.0	34.0	30.0	34.0
4	30.311467318826274	35.0	33.0	37.0	0.0	37.0
5	32.93810795820055	35.0	33.0	37.0	19.0	37.0
6	34.60364314943668	36.0	33.0	37.0	31.0	37.0
7	35.5335923975595	37.0	35.0	37.0	33.0	37.0
8	35.716546602686265	37.0	35.0	37.0	35.0	37.0
9	37.74010915884035	39.0	37.0	39.0	35.0	39.0
10	37.238233998520016	39.0	37.0	39.0	34.0	39.0
11	36.8230560446305	39.0	35.0	39.0	33.0	39.0
12	35.786488571762405	37.0	35.0	39.0	32.0	39.0
13	35.37278422192766	37.0	35.0	39.0	30.0	39.0
14	36.24847771228426	38.0	35.0	40.0	31.0	41.0
15	36.54757434498652	38.0	35.0	40.0	31.0	41.0
16	36.7216133966691	38.0	35.0	40.0	32.0	41.0
17	36.688651872205725	38.0	35.0	40.0	32.0	41.0
18	36.67220888177996	38.0	35.0	40.0	32.0	41.0
19	36.640626280044536	38.0	35.0	40.0	31.0	41.0
20	36.53288833109724	38.0	35.0	40.0	31.0	41.0
21	36.36821836602861	38.0	35.0	40.0	31.0	41.0
22	36.22231947762768	37.0	35.0	40.0	31.0	41.0
23	36.19193046495918	37.0	35.0	40.0	31.0	41.0
24	36.13405096407079	37.0	35.0	40.0	31.0	41.0
25	36.01075270983329	37.0	34.0	40.0	31.0	41.0
26	35.82747652104544	37.0	34.0	40.0	30.0	41.0
27	35.84136815188765	37.0	34.0	40.0	30.0	41.0
28	35.87199252487567	37.0	34.0	40.0	30.0	41.0
29	35.931428559917165	36.0	35.0	40.0	30.0	41.0
30	35.9002330772237	36.0	35.0	40.0	30.0	41.0
31	35.79509785751602	36.0	34.0	40.0	30.0	41.0
32	35.6949189702432	36.0	34.0	40.0	30.0	41.0
33	35.54291716525451	36.0	34.0	40.0	30.0	41.0
34	35.46852450231869	36.0	34.0	40.0	30.0	41.0
35	35.33295695827172	36.0	34.0	40.0	29.0	41.0
36	35.15729355106009	36.0	34.0	40.0	28.0	41.0
37	35.11753713052459	36.0	34.0	40.0	28.0	41.0
38	35.11600208433219	35.0	34.0	40.0	29.0	41.0
39	35.05157748491478	36.0	34.0	40.0	28.0	41.0
40	34.875283204607015	35.0	34.0	40.0	27.0	41.0
41	34.82533611871024	35.0	34.0	40.0	27.0	41.0
42	34.7912129013716	35.0	33.0	40.0	27.0	41.0
43	34.74889907776359	35.0	33.0	40.0	27.0	41.0
44	34.67274070880725	35.0	34.0	40.0	27.0	41.0
45	34.57415125912735	35.0	33.0	40.0	27.0	41.0
46	34.49840082567489	35.0	33.0	39.0	27.0	41.0
47	34.43948822392604	35.0	33.0	39.0	27.0	41.0
48	34.34239621650732	35.0	33.0	39.0	27.0	41.0
49	34.302451440566315	35.0	33.0	39.0	27.0	41.0
50	34.1524594314017	35.0	33.0	39.0	26.0	41.0
51	34.0504137775432	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	6.0
14	15.0
15	58.0
16	160.0
17	392.0
18	967.0
19	2026.0
20	3440.0
21	6024.0
22	9288.0
23	13571.0
24	19830.0
25	27741.0
26	36484.0
27	45645.0
28	55959.0
29	69643.0
30	87142.0
31	110937.0
32	144794.0
33	215588.0
34	329033.0
35	449504.0
36	230517.0
37	293890.0
38	375298.0
39	450153.0
40	300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.335049684194125	25.108749226097665	30.316390076322552	12.239811013385657
2	28.263886930350807	27.381604694045013	30.464589855265157	13.889918520339025
3	29.666379265192322	25.58997882092874	30.793254929136733	13.950386984742206
4	23.414759274405288	39.00467766044456	25.13241955780463	12.448143507345526
5	24.08155755483123	33.08078264524854	28.02973530861412	14.807924491306105
6	23.134341387289602	40.789857145351284	24.975557444705434	11.100244022653683
7	90.07602037595873	4.208820002068216	3.870988969961174	1.8441706520118772
8	91.65760143324698	3.000592261916753	3.5990991172477145	1.742707187588554
9	87.56501786858232	4.828512643650375	4.993902791151795	2.612566696615512
10	57.89884005302154	22.46942330342478	9.363916073397501	10.267820570156177
11	50.95540845255794	20.875755268243616	14.99960381572462	13.169232463473824
12	46.92144673067393	20.532854420409265	18.469875893596992	14.075822955319813
13	23.164928156346402	39.63578578114781	19.7398815207567	17.459404541749095
14	15.497688029728593	43.307473915630204	23.774649041166903	17.420189013474292
15	15.39602311567372	23.479055281807888	45.581336631735304	15.543584970783089
16	16.89611779700055	18.27551057409116	45.8962024058458	18.93216922306249
17	17.63077102831979	19.001434321376625	25.973740369028864	37.39405428127472
18	23.146294065428155	25.430632162373772	29.271873736742936	22.151200035455133
19	33.855222178798634	25.07547646195355	20.695357123191823	20.373944236055994
20	37.95049173855061	23.265955146568036	20.69743876938449	18.086114345496863
21	26.913704349834745	27.54226077520504	23.478249483281697	22.06578539167852
22	24.467333599246846	26.119556327331477	21.586469568347162	27.82664050507452
23	24.781057825445238	30.068741329272108	21.57132727104242	23.578873574240234
24	23.426241903403557	23.11070463052123	33.59622510250429	19.866828363570924
25	21.270126496938637	24.68741732171372	31.7116973743055	22.33075880704214
26	20.282351803578553	36.8385233473408	22.796543930121153	20.0825809189595
27	22.551043979140562	34.03491525014001	25.512790037107024	17.901250733612407
28	18.596285537393754	30.150865629066764	34.14567897255316	17.107169860986325
29	19.52993742974444	25.778938575321348	33.574602842051405	21.11652115288281
30	22.087609101762954	29.652076341352373	26.777692273600834	21.482622283283845
31	31.47244236190292	26.62009151185262	20.83996438370514	21.067501742539314
32	34.623248899077765	26.475014202199027	21.80420304511263	17.09753385361058
33	30.78150370062973	28.17081720057534	21.18770002269666	19.85997907609827
34	22.650157197862487	27.62811189318335	24.870770061361558	24.85096084759261
35	23.697090933020686	23.76712825492242	27.893018158669786	24.642762653387106
36	31.71636429076971	25.76691874730561	22.913854765559634	19.60286219636504
37	24.512693341283878	31.93634728842081	25.331787543160583	18.21917182713473
38	23.267835343129157	31.520723123597406	21.020530403449893	24.190911129823544
39	24.473108488684574	30.611446636664102	23.8189679601076	21.096476914543725
40	27.25123320749446	24.531327432202126	24.77038099497316	23.44705836533025
41	18.414544394798302	24.87852587217618	26.74472168390404	29.96220804912148
42	24.12141100693927	25.55267706415365	22.79771905297185	27.528192875935233
43	24.506817727030377	26.557742850888328	23.544660711815556	25.39077871026574
44	22.424567185466618	30.240980764246185	26.09185700299354	21.24259504729366
45	20.0406122457202	34.502916319166054	20.348125108279177	25.108346326834567
46	22.398311583488116	33.06379372632127	22.89082235768591	21.647072332504706
47	23.863051854478158	26.348638133340852	25.35673372253402	24.431576289646966
48	25.582693059254396	21.25434627580066	29.672892803279062	23.49006786166588
49	21.710629691258294	24.09223438530331	29.982117987706204	24.215017935732195
50	20.12817568556667	32.81648072865675	23.80634378319722	23.248999802579362
51	19.021444984777123	34.25117142960746	21.76263727113643	24.96474631447899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	489.0
1	1092.0
2	1695.0
3	4802.0
4	7909.0
5	5885.5
6	3862.0
7	4129.5
8	4397.0
9	4741.5
10	5086.0
11	5163.0
12	5240.0
13	5100.0
14	4960.0
15	4788.0
16	4616.0
17	4493.0
18	4370.0
19	4462.5
20	4555.0
21	5169.0
22	5783.0
23	5857.0
24	5931.0
25	8423.0
26	15527.0
27	20139.0
28	26145.0
29	32151.0
30	36310.5
31	40470.0
32	44652.5
33	48835.0
34	51101.0
35	53367.0
36	57203.0
37	61039.0
38	83075.5
39	105112.0
40	153203.0
41	201294.0
42	246131.5
43	290969.0
44	289191.0
45	287413.0
46	263009.5
47	238606.0
48	229121.0
49	219636.0
50	204277.0
51	188918.0
52	180959.0
53	173000.0
54	159423.0
55	145846.0
56	147587.0
57	149328.0
58	158631.5
59	167935.0
60	163006.0
61	158077.0
62	134867.0
63	111657.0
64	94032.0
65	76407.0
66	61349.5
67	46292.0
68	41241.5
69	36191.0
70	29723.0
71	23255.0
72	18604.5
73	13954.0
74	11906.5
75	7487.5
76	5116.0
77	3656.5
78	2197.0
79	1469.0
80	741.0
81	694.5
82	648.0
83	371.0
84	94.0
85	68.0
86	42.0
87	27.0
88	12.0
89	8.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2978412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.561233715480332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75332198984395	11.32184293649114
2	9.3741727824029	2.729990415477263
3	3.143220090310021	1.3730748706259224
4	1.5255576347336393	0.8885600506316758
5	0.8747762497462364	0.636891071065317
6	0.5569972660348991	0.48663404217706446
7	0.37953259584484333	0.38685239815177924
8	0.2744988742249105	0.31976338097801293
9	0.219274891732676	0.28736216518105273
>10	2.432060515886621	8.68959893399128
>50	1.2816069966580383	13.680327833725189
>100	2.1632967189077945	56.46650862733126
>500	0.01840567137399171	1.7487054983492734
>1k	0.003277722299477976	0.9838877758237741
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3946	0.13248670768181164	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3708	0.12449587229704957	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3309	0.11109947179906608	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0497580589925101	0.0
2	0.0	0.0	0.0	0.17865224824503795	0.0
3	0.0	0.0	0.0	0.25862775196984167	0.0
4	0.0	0.0	0.0	0.397157948598112	0.0
5	0.0	0.0	0.0	0.6735804180214154	0.0
6	0.0	0.0	0.0	0.9268361798166271	0.0
7	0.0	0.0	0.0	1.0763453813642976	0.0
8	0.0	0.0	0.0	1.3859063151773496	0.0
9	0.0	0.0	0.0	1.484515909820401	0.0
10	0.0	0.0	0.0	1.6495031580587238	0.0
11	0.0	0.0	0.0	1.9165246446764248	0.0
12	0.0	0.0	0.0	2.12243974305771	0.0
13	0.0	0.0	0.0	2.2185312173064036	0.0
14	0.0	0.0	0.0	2.2579146202741596	0.0
15	0.0	0.0	0.0	2.322412077308311	0.0
16	0.0	0.0	0.0	2.466381413988394	0.0
17	0.0	0.0	0.0	2.62233700374562	0.0
18	0.0	0.0	0.0	2.8024665492886816	0.0
19	0.0	0.0	0.0	2.909234854009452	0.0
20	3.357493859143731E-5	0.0	0.0	3.02738506291272	0.0
21	3.357493859143731E-5	0.0	0.0	3.183307077731355	0.0
22	3.357493859143731E-5	0.0	0.0	3.3456419058209543	0.0
23	3.357493859143731E-5	0.0	0.0	3.5191571884615023	0.0
24	3.357493859143731E-5	0.0	0.0	3.6492936504419133	0.0
25	3.357493859143731E-5	0.0	0.0	3.75643127948719	0.0
26	3.357493859143731E-5	0.0	0.0	3.8605807389978284	0.0
27	3.357493859143731E-5	0.0	0.0	3.961574154280872	0.0
28	3.357493859143731E-5	0.0	0.0	4.075561070798802	0.0
29	6.714987718287463E-5	0.0	0.0	4.1902194860885595	0.0
30	1.0072481577431195E-4	0.0	0.0	4.333685198689771	0.0
31	1.3429975436574925E-4	0.0	0.0	4.485746095570391	0.0
32	1.3429975436574925E-4	0.0	0.0	4.623235469102327	0.0
33	1.3429975436574925E-4	0.0	0.0	4.751055260319929	0.0
34	1.6787469295718657E-4	0.0	0.0	4.897475567517187	0.0
35	1.6787469295718657E-4	0.0	0.0	5.069211378412389	0.0
36	1.6787469295718657E-4	0.0	0.0	5.234769400606766	0.0
37	1.6787469295718657E-4	0.0	0.0	5.39975664884509	0.0
38	1.6787469295718657E-4	0.0	0.0	5.54926585039276	0.0
39	1.6787469295718657E-4	0.0	0.0	5.701729646536476	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	105	0.0	45.000004	2
CGGTATG	20	7.035096E-4	45.000004	24
CACGATA	35	1.212793E-7	45.0	13
AACGCAC	35	1.212793E-7	45.0	40
TTACGAG	55	1.8189894E-12	45.0	1
TAAATCG	35	1.212793E-7	45.0	1
CGCGTAA	35	1.212793E-7	45.0	31
TCGTAAG	155	0.0	43.548386	1
GTCGATG	105	0.0	42.857147	1
GTTTACG	150	0.0	42.0	1
TTAAACG	60	3.6379788E-12	41.249996	1
TATCGTG	60	3.6379788E-12	41.249996	1
CGTAACG	55	6.184564E-11	40.909092	41
TCGGTAC	150	0.0	40.5	36
TCTCAAC	670	0.0	40.298508	35
CGGGTAT	330	0.0	40.227272	6
TTAGTAG	500	0.0	40.05	1
ACGCATT	45	1.9301297E-8	40.0	9
CTATACG	45	1.9301297E-8	40.0	1
TATCGTT	45	1.9301297E-8	40.0	23
>>END_MODULE