FastQCFastQC Report
Thu 26 May 2016
SRR1545311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545311_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2166219
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22940.10589880339891766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA351.2125929E-745.00000435
ACGTTAG302.1663946E-645.0000041
ATAGCCG207.034669E-445.0000042
GAACGTA351.2125929E-745.0000049
CACGTTA302.1663946E-645.00000438
CACGTGC302.1663946E-645.00000437
CAGTACG700.045.0000041
TACGTTC207.034669E-445.00000431
CGAATCT253.8919534E-545.038
TAGTCCG253.8919534E-545.024
TAACTCG253.8919534E-545.038
AAGTACG603.6379788E-1241.2500041
TACGGGT603.6379788E-1241.2500044
CGAATAT2500.040.49999614
GGACTAA5600.040.1785748
CTATGCG451.929584E-840.01
ATACGTA451.929584E-840.036
TGTCCGA451.929584E-840.041
CTACCTT3500.039.85714317
GACTAAT5300.039.4811339
Produced by FastQC (version 0.11.5)