FastQCFastQC Report
Thu 26 May 2016
SRR1545310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545310_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1985278
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC406.8193913E-945.00000436
AGGTAAC406.8193913E-945.00000419
CCTTACC406.8193913E-945.00000416
TCGTTAC207.034527E-445.00000415
ACACGTG406.8193913E-945.00000421
CGTATTA207.034527E-445.00000421
GTCGATA207.034527E-445.00000421
GGTAATC406.8193913E-945.0000048
CGAGTTA406.8193913E-945.00000422
CGTGCTA207.034527E-445.00000412
ACGTTGT207.034527E-445.00000427
CGTTGTA207.034527E-445.00000428
CAGTTAC650.045.00000439
AACCACG207.034527E-445.00000437
CCGTGCA207.034527E-445.00000433
GCAAACG1250.045.0000041
CCTACGG207.034527E-445.00000433
TTGTACC207.034527E-445.00000430
TATGCGT207.034527E-445.00000421
TCTAGGC406.8193913E-945.00000413