FastQCFastQC Report
Thu 26 May 2016
SRR1545309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545309_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4906009
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62190.12676291462164052No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG207.035543E-445.0000041
CGTCGAT207.035543E-445.00000441
TAGTCCG207.035543E-445.00000413
ACGTTAG1500.045.01
TAAGACG1650.040.9090921
GTCGATA501.0822987E-940.530
CGTTTTT46350.039.4174771
TACGGGA9250.038.6756744
AGGGCGA35750.038.391616
AACGTTC1700.038.3823539
CGAATAT4750.038.3684214
CGTTAGG6950.038.201442
CGAACGT1650.038.1818237
TTACGAG2550.037.9411771
CGACCCG18700.037.9010732
ATAGGGC24600.037.5914654
ATTGGGA28500.037.578954
TCGACGA301.14046576E-437.50000428
AATGGGC16150.037.3374634
TAGGGAT45500.037.3351675
Produced by FastQC (version 0.11.5)