FastQCFastQC Report
Thu 26 May 2016
SRR1545308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545308_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4264263
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46080.10806087710818962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG800.045.01
TCGTAAT351.2129567E-745.013
CAATACG207.035442E-445.01
CTTACGT253.892591E-545.022
GCGATAT8350.042.5748529
ACGGGTC2500.042.3000035
CGTTACA603.6379788E-1241.25000435
CGGTCTA2300.041.08695631
TGTACGA556.184564E-1140.90909234
GACCGTT1050.040.7142879
GCACCGA18550.040.269549
ACCGTTG950.040.26315710
CGTATTT6800.040.0367661
ACGATTG900.040.0000041
CGGAATA403.461173E-739.37539
CTATCGT403.461173E-739.37541
CATTCTA14000.039.37545
TCGCTAA403.461173E-739.37515
GCTCGAC403.461173E-739.37520
TATAGCG3900.039.230771