##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545308_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4264263
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24147478708513	31.0	31.0	33.0	30.0	34.0
2	31.559378490491792	31.0	31.0	34.0	30.0	34.0
3	31.669568692174945	31.0	31.0	34.0	30.0	34.0
4	30.29776681222523	35.0	33.0	37.0	0.0	37.0
5	32.85608673761445	35.0	33.0	37.0	19.0	37.0
6	34.474724002717466	35.0	33.0	37.0	30.0	37.0
7	35.476117209468555	36.0	35.0	37.0	33.0	37.0
8	35.64711182213667	37.0	35.0	37.0	35.0	37.0
9	37.64016103134352	39.0	37.0	39.0	35.0	39.0
10	37.083379472607575	39.0	37.0	39.0	33.0	39.0
11	36.68068386025909	39.0	35.0	39.0	32.0	39.0
12	35.681896027519876	37.0	35.0	39.0	31.0	39.0
13	35.253218199721736	37.0	35.0	39.0	30.0	39.0
14	36.10346571025286	38.0	35.0	40.0	30.0	41.0
15	36.39925656555423	38.0	35.0	40.0	31.0	41.0
16	36.583295636315114	38.0	35.0	40.0	32.0	41.0
17	36.52112287633291	38.0	35.0	40.0	31.0	41.0
18	36.514917114633874	38.0	35.0	40.0	31.0	41.0
19	36.498591667540204	38.0	35.0	40.0	31.0	41.0
20	36.40252817427068	38.0	35.0	40.0	31.0	41.0
21	36.24354313981103	37.0	35.0	40.0	31.0	41.0
22	36.11293979756877	37.0	34.0	40.0	30.0	41.0
23	36.05798258690892	37.0	34.0	40.0	30.0	41.0
24	35.961564518886384	37.0	34.0	40.0	30.0	41.0
25	35.84319564717279	37.0	34.0	40.0	30.0	41.0
26	35.64951669256798	37.0	34.0	40.0	30.0	41.0
27	35.66648586168348	37.0	34.0	40.0	30.0	41.0
28	35.68660235074619	37.0	34.0	40.0	30.0	41.0
29	35.727884748196814	36.0	34.0	40.0	30.0	41.0
30	35.67727975502449	36.0	34.0	40.0	30.0	41.0
31	35.58941509939701	36.0	34.0	40.0	30.0	41.0
32	35.4889921189195	36.0	34.0	40.0	29.0	41.0
33	35.36178866078382	36.0	34.0	40.0	29.0	41.0
34	35.28660099060494	36.0	34.0	40.0	29.0	41.0
35	35.16780484693369	36.0	34.0	40.0	28.0	41.0
36	35.00365877995799	36.0	34.0	40.0	27.0	41.0
37	34.962614876240046	36.0	33.0	40.0	27.0	41.0
38	34.93827679015108	36.0	33.0	40.0	27.0	41.0
39	34.88318684846596	36.0	34.0	40.0	27.0	41.0
40	34.7381725751906	36.0	33.0	40.0	27.0	41.0
41	34.68715297344465	35.0	33.0	40.0	26.0	41.0
42	34.646215301448336	35.0	33.0	40.0	27.0	41.0
43	34.57087613967525	35.0	33.0	40.0	27.0	41.0
44	34.50307755408144	35.0	33.0	40.0	27.0	41.0
45	34.407763545541165	35.0	33.0	40.0	26.0	41.0
46	34.334419570275095	35.0	33.0	39.0	26.0	41.0
47	34.27160426080661	35.0	33.0	39.0	26.0	41.0
48	34.174886023681	35.0	33.0	39.0	26.0	41.0
49	34.137400765384314	35.0	33.0	39.0	26.0	41.0
50	33.98493713919615	35.0	33.0	39.0	26.0	41.0
51	33.89761489851822	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	8.0
13	13.0
14	32.0
15	91.0
16	278.0
17	794.0
18	1916.0
19	3683.0
20	6575.0
21	10791.0
22	16476.0
23	24206.0
24	33909.0
25	45861.0
26	59750.0
27	73964.0
28	88953.0
29	107637.0
30	133248.0
31	167738.0
32	211965.0
33	303251.0
34	451824.0
35	605825.0
36	328167.0
37	408047.0
38	528911.0
39	649917.0
40	430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.43080926293711	25.572648778933193	30.093711386938377	11.902830571191316
2	28.352894744062457	26.017930882780917	32.02459604391193	13.604578329244701
3	29.54226791358788	26.11262485451765	30.02284333775848	14.322263894135986
4	22.572388241532007	39.25252734177043	25.41414542208114	12.760938994616419
5	24.182725127413576	33.16854518588558	28.245420134733717	14.403309551967128
6	22.368460857128184	41.52403357860432	25.15027801990637	10.957227544361125
7	89.11221001143691	4.541417825307679	4.269952392711238	2.076419770544171
8	91.10301123547023	3.1434505798540098	3.6091817038489418	2.144356480826816
9	86.9439807066309	5.04302853740494	5.015286346081375	2.9977044098827865
10	56.306845989564906	23.41539440695848	9.836353902186614	10.44140570129
11	49.63136654563755	20.16369534430686	16.636004861801442	13.568933248254153
12	45.837604294106626	20.857039070995388	19.233757392543566	14.071599242354424
13	23.368422632468963	39.39839076529754	19.969851765709574	17.26333483652392
14	15.8998401365019	41.41688727923207	24.42984872180726	18.253423862458764
15	15.724757126847008	24.05079142632619	43.99252578933335	16.231925657493452
16	17.564910982272906	18.779704722715273	43.700376829477925	19.955007465533903
17	17.488766523077963	20.31260736028711	26.882300646090545	35.316325470544385
18	23.010916540560466	25.032227139836355	29.440421474941857	22.51643484466132
19	31.88947304610433	26.31648188678794	21.108477596245823	20.685567470861905
20	36.12474183698332	22.797632322396623	21.790189770190064	19.28743607042999
21	25.39046020379137	28.712511399976968	23.017623443957373	22.879404952274285
22	24.384002581454286	26.535253571367434	22.173726151506134	26.90701769567215
23	24.768054878416272	30.15686415214071	21.606406546688138	23.46867442275488
24	23.596246291563162	23.06285986581972	33.83606967956714	19.50482416304998
25	20.866677313289543	25.57063201777189	30.316540044551658	23.24615062438691
26	19.642034274152415	36.60841275502942	23.426392790500962	20.32316018031721
27	21.307761739836405	33.77610621108501	25.35003117772051	19.566100871358074
28	18.099188535041108	30.0911318087088	34.25288262004478	17.556797036205317
29	18.239259632907256	25.46505691604856	34.04651636167844	22.249167089365734
30	21.0536263828005	30.2090888859341	27.064348516965296	21.672936214300105
31	30.60306083372437	27.605684733798082	20.664133520845223	21.12712091163233
32	32.61918882582992	26.938699606473616	21.8355434456083	18.606568122088156
33	29.131692862283586	28.143878555333007	21.817322243023003	20.9071063393604
34	21.090420548638768	27.41317784573794	25.966222064633442	25.53017954098985
35	22.563570774129083	24.51349740857916	28.07007916725587	24.852852650035892
36	29.625775896092716	25.71049205923743	23.503991193788938	21.159740850880915
37	22.908296228445572	31.690306156069642	26.39698817826199	19.004409437222797
38	21.661797126490555	31.985832018334705	22.563172112039055	23.789198743135685
39	24.195857525673254	30.107430052977502	24.09703622876919	21.599676192580052
40	25.72015375224277	25.47675882092638	24.638348994890794	24.164738431940055
41	18.172472007472333	24.564479254680116	26.70712383359094	30.55592490425661
42	23.709184916596374	26.09545893393536	22.029527728472658	28.16582842099561
43	23.521204015793586	27.697611521615812	23.47254379009925	25.308640672491357
44	21.766715608300895	30.541995181816883	25.604025830489345	22.087263379392873
45	19.737549020780378	34.45737282151688	20.864027382926427	24.941050774776322
46	21.618342958677736	33.06754297284197	22.883508826730434	22.430605241749866
47	23.72850830260704	26.01213855711995	25.88960859121494	24.369744549058066
48	24.43303801852747	21.7708663841794	29.62554138898093	24.170554208312197
49	20.763283127705773	24.941918451089904	29.739441493172443	24.555356928031877
50	19.273600150834973	32.09595655802656	25.483559527167998	23.146883763970468
51	18.60079924713837	32.86251340501278	23.0677845151671	25.468902832681756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	641.0
1	1382.0
2	2123.0
3	5916.0
4	9709.0
5	7343.0
6	4977.0
7	5356.0
8	5735.0
9	6131.0
10	6527.0
11	6529.5
12	6532.0
13	6584.5
14	6637.0
15	6869.0
16	7101.0
17	7240.0
18	7379.0
19	7291.0
20	7203.0
21	8103.5
22	9004.0
23	9377.5
24	9751.0
25	13933.5
26	24117.5
27	30119.0
28	39499.0
29	48879.0
30	54719.0
31	60559.0
32	65590.0
33	70621.0
34	82583.5
35	94546.0
36	98164.0
37	101782.0
38	135970.0
39	170158.0
40	239251.5
41	308345.0
42	359147.0
43	409949.0
44	406844.5
45	403740.0
46	376084.5
47	348429.0
48	329962.0
49	311495.0
50	292202.0
51	272909.0
52	255741.0
53	238573.0
54	226342.0
55	214111.0
56	211225.0
57	208339.0
58	212804.5
59	217270.0
60	212470.5
61	207671.0
62	180117.0
63	152563.0
64	125454.5
65	98346.0
66	83731.5
67	69117.0
68	57992.0
69	46867.0
70	38842.5
71	30818.0
72	26937.0
73	23056.0
74	17504.5
75	9587.0
76	7221.0
77	5348.5
78	3476.0
79	2341.5
80	1207.0
81	834.0
82	461.0
83	308.0
84	155.0
85	97.0
86	39.0
87	36.5
88	34.0
89	20.0
90	6.0
91	5.5
92	5.0
93	3.0
94	1.0
95	3.5
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4264263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.405803408998649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37279375281851	11.14617256008102
2	9.595164183978891	2.7645209782292968
3	3.4139644761392076	1.4754270326569947
4	1.7795726541329322	1.0254469522987588
5	1.0775869588332154	0.776175294252601
6	0.7018934445870986	0.6066803386071978
7	0.49875269477255935	0.5029453289421257
8	0.3639713599938693	0.41946398868620455
9	0.27965834830666103	0.3625832868651926
>10	2.094581749094002	6.684565186339175
>50	0.8090136608566356	8.581615958023866
>100	1.975557526565249	61.34406924951106
>500	0.03286966888824595	3.0136477832201307
>1k	0.004619521032942674	1.2966860622863128
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4608	0.10806087710818962	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3450711177992538E-5	0.0	0.0	0.05656311536131801	0.0
2	2.3450711177992538E-5	0.0	0.0	0.19527407197914387	0.0
3	2.3450711177992538E-5	0.0	0.0	0.2778440260368556	0.0
4	2.3450711177992538E-5	0.0	0.0	0.4600091504675016	0.0
5	2.3450711177992538E-5	0.0	0.0	0.795119813201015	0.0
6	2.3450711177992538E-5	0.0	0.0	1.096719409661177	0.0
7	2.3450711177992538E-5	0.0	0.0	1.2675343898816747	0.0
8	2.3450711177992538E-5	0.0	0.0	1.6919453607809838	0.0
9	4.6901422355985076E-5	0.0	0.0	1.8190013139433474	0.0
10	4.6901422355985076E-5	0.0	0.0	2.0479506071740885	0.0
11	4.6901422355985076E-5	0.0	0.0	2.369905420936748	0.0
12	4.6901422355985076E-5	0.0	0.0	2.6414881070890797	0.0
13	7.035213353397762E-5	0.0	0.0	2.74732116663536	0.0
14	7.035213353397762E-5	0.0	0.0	2.7866714599920313	0.0
15	9.380284471197015E-5	0.0	0.0	2.8524741555574784	0.0
16	9.380284471197015E-5	0.0	0.0	3.0065453279968897	0.0
17	9.380284471197015E-5	0.0	0.0	3.1828477746330375	0.0
18	9.380284471197015E-5	0.0	0.0	3.3872441732604206	0.0
19	9.380284471197015E-5	0.0	0.0	3.516926606074719	0.0
20	1.1725355588996269E-4	0.0	0.0	3.6426927701222933	0.0
21	1.1725355588996269E-4	0.0	0.0	3.8176585262212956	0.0
22	1.4070426706795525E-4	0.0	0.0	4.006671258315915	0.0
23	1.6415497824594778E-4	0.0	0.0	4.207151388176573	0.0
24	1.876056894239403E-4	0.0	0.0	4.362770307553732	0.0
25	2.1105640060193286E-4	0.0	0.0	4.485909991949371	0.0
26	2.1105640060193286E-4	0.0	0.0	4.600982631699781	0.0
27	2.1105640060193286E-4	0.0	0.0	4.707214353336086	0.0
28	2.579578229579179E-4	0.0	0.0	4.830682347688217	0.0
29	2.579578229579179E-4	0.0	0.0	4.9636947814897905	0.0
30	2.579578229579179E-4	0.0	0.0	5.126114407108568	0.0
31	2.579578229579179E-4	0.0	0.0	5.290879103845143	0.0
32	2.579578229579179E-4	0.0	0.0	5.446122811843454	0.0
33	2.579578229579179E-4	0.0	0.0	5.585279331973661	0.0
34	3.2830995649189555E-4	0.0	0.0	5.7482852253718875	0.0
35	3.2830995649189555E-4	0.0	0.0	5.941495634767368	0.0
36	3.2830995649189555E-4	0.0	0.0	6.1200024482542466	0.0
37	3.2830995649189555E-4	0.0	0.0	6.301112290681884	0.0
38	3.2830995649189555E-4	0.0	0.0	6.464962409682517	0.0
39	3.2830995649189555E-4	0.0	0.0	6.621636611062685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	80	0.0	45.0	1
TCGTAAT	35	1.2129567E-7	45.0	13
CAATACG	20	7.035442E-4	45.0	1
CTTACGT	25	3.892591E-5	45.0	22
GCGATAT	835	0.0	42.574852	9
ACGGGTC	250	0.0	42.300003	5
CGTTACA	60	3.6379788E-12	41.250004	35
CGGTCTA	230	0.0	41.086956	31
TGTACGA	55	6.184564E-11	40.909092	34
GACCGTT	105	0.0	40.714287	9
GCACCGA	1855	0.0	40.26954	9
ACCGTTG	95	0.0	40.263157	10
CGTATTT	680	0.0	40.036766	1
ACGATTG	90	0.0	40.000004	1
CGGAATA	40	3.461173E-7	39.375	39
CTATCGT	40	3.461173E-7	39.375	41
CATTCTA	1400	0.0	39.375	45
TCGCTAA	40	3.461173E-7	39.375	15
GCTCGAC	40	3.461173E-7	39.375	20
TATAGCG	390	0.0	39.23077	1
>>END_MODULE