FastQCFastQC Report
Thu 26 May 2016
SRR1545307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545307_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2076598
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23260.11201012425129948No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.10252345422657637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG406.8193913E-945.0000041
CCACTAG406.8193913E-945.0000041
CTACGAT207.0346013E-445.00000419
CGCTAAC406.8193913E-945.00000437
CGTTTAG207.0346013E-445.00000430
TGGTCGA207.0346013E-445.00000426
TACGTCT207.0346013E-445.00000436
TACGTCG207.0346013E-445.00000437
ACTATCG253.891898E-545.011
CGAACTA253.891898E-545.028
CGCATTG253.891898E-545.039
GTTAGTA253.891898E-545.022
CATTCGA253.891898E-545.031
GTGTCGA453.8562575E-1045.014
GTACTCG253.891898E-545.01
ACTCGTT253.891898E-545.032
CATCGTA351.2125565E-745.018
ACCGTAT253.891898E-545.06
ATACGTC351.2125565E-745.037
GCTCGAT253.891898E-545.019