##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545307_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2076598
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339623749998797	31.0	31.0	33.0	30.0	34.0
2	31.66403367430769	31.0	31.0	34.0	30.0	34.0
3	31.75771526313711	31.0	31.0	34.0	30.0	34.0
4	30.30412915740071	35.0	33.0	37.0	0.0	37.0
5	32.90153414382562	35.0	33.0	37.0	19.0	37.0
6	34.54913565360267	35.0	33.0	37.0	30.0	37.0
7	35.49710199085234	36.0	35.0	37.0	33.0	37.0
8	35.68802676300372	37.0	35.0	37.0	35.0	37.0
9	37.73240752422954	39.0	37.0	39.0	35.0	39.0
10	37.220097967926385	39.0	37.0	39.0	34.0	39.0
11	36.80219185417688	39.0	35.0	39.0	33.0	39.0
12	35.58002848890349	37.0	35.0	39.0	31.0	39.0
13	35.111174141552674	37.0	35.0	39.0	30.0	39.0
14	35.93288831059262	37.0	35.0	40.0	30.0	41.0
15	36.26991984004608	37.0	35.0	40.0	31.0	41.0
16	36.485581706232985	37.0	35.0	40.0	32.0	41.0
17	36.41489397562744	37.0	35.0	40.0	32.0	41.0
18	36.394310309458064	37.0	35.0	40.0	31.0	41.0
19	36.36957225230883	37.0	35.0	40.0	31.0	41.0
20	36.25369233717841	37.0	35.0	40.0	31.0	41.0
21	36.08412701928828	36.0	34.0	40.0	31.0	41.0
22	35.95942257480745	36.0	34.0	40.0	30.0	41.0
23	35.93360294096402	36.0	34.0	40.0	31.0	41.0
24	35.837432184756025	36.0	34.0	40.0	30.0	41.0
25	35.731964973480665	36.0	34.0	40.0	30.0	41.0
26	35.55957628775526	35.0	34.0	40.0	30.0	41.0
27	35.55432442870502	36.0	34.0	40.0	30.0	41.0
28	35.604961095021764	36.0	34.0	40.0	30.0	41.0
29	35.666422677860616	36.0	34.0	40.0	30.0	41.0
30	35.64485567259528	36.0	34.0	40.0	30.0	41.0
31	35.511182713264674	36.0	34.0	40.0	30.0	41.0
32	35.39715486579492	35.0	34.0	40.0	30.0	41.0
33	35.25973443102613	35.0	34.0	40.0	29.0	41.0
34	35.18687873146367	35.0	34.0	40.0	29.0	41.0
35	35.07237414270841	35.0	34.0	40.0	29.0	41.0
36	34.866481138862696	35.0	34.0	40.0	27.0	41.0
37	34.792085420481	35.0	33.0	40.0	27.0	41.0
38	34.79467330701465	35.0	33.0	40.0	27.0	41.0
39	34.75530121862778	35.0	33.0	40.0	27.0	41.0
40	34.60415978441663	35.0	33.0	40.0	27.0	41.0
41	34.606459218394704	35.0	34.0	40.0	27.0	41.0
42	34.572266273973106	35.0	33.0	40.0	27.0	41.0
43	34.49352932055217	35.0	33.0	39.0	27.0	41.0
44	34.4245689343821	35.0	33.0	39.0	27.0	41.0
45	34.32783427509802	35.0	33.0	39.0	27.0	41.0
46	34.29344389236626	35.0	33.0	39.0	27.0	41.0
47	34.23059157333292	35.0	33.0	39.0	27.0	41.0
48	34.146727965643805	35.0	33.0	39.0	27.0	41.0
49	34.10434951781712	35.0	33.0	39.0	26.0	41.0
50	33.929953221567196	35.0	33.0	38.0	26.0	40.0
51	33.840712550045794	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	3.0
14	9.0
15	36.0
16	110.0
17	346.0
18	802.0
19	1614.0
20	2832.0
21	4769.0
22	7332.0
23	10823.0
24	15461.0
25	21428.0
26	28113.0
27	34327.0
28	41805.0
29	51246.0
30	64038.0
31	81265.0
32	105617.0
33	156890.0
34	242677.0
35	334176.0
36	150303.0
37	187328.0
38	241569.0
39	291437.0
40	237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.806348652941015	26.03041127844677	30.231561428836972	11.931678639775248
2	28.213741899009825	26.904340657171005	31.13597335642238	13.745944087396792
3	29.06388236914415	27.231606695181256	30.06855443374211	13.635956501932487
4	23.25308027841691	38.41711298961089	25.20569701020612	13.12410972176608
5	24.49727872221778	32.790265617129556	29.00436194198396	13.708093718668707
6	22.052173795794854	41.096784259640046	25.569946614607158	11.28109532995794
7	89.80630820216527	4.183765947959114	4.197345851243235	1.8125799986323785
8	92.02199944332028	2.872486634389516	3.417127436316514	1.6883864859736937
9	88.6289016940207	4.377737048769189	4.477371161871484	2.5159900953386263
10	61.517202655497115	20.38401269769113	8.506990760850199	9.591793885961557
11	54.64215991732632	18.303783399579505	14.31167707953104	12.742379603563137
12	48.386543760516	20.412569019136107	17.677566866576967	13.523320353770925
13	21.755823707814415	43.422896487428	18.943965081349397	15.87731472340819
14	14.675396971392635	45.213228559403404	24.137507596559374	15.973866872644585
15	14.18680938727669	22.21975558100316	48.97365787696993	14.61977715475022
16	16.72678101394685	16.142508082931794	47.27790357112932	19.852807331992036
17	17.500450255658535	17.764054477563786	25.410792074344673	39.324703192433006
18	22.6517602347686	24.705359438851428	30.729780150033854	21.91310017634612
19	34.09225088341605	24.31857297368099	20.43491325716388	21.15426288573908
20	37.57068050725273	23.256403020709833	19.741471387336404	19.431445084701036
21	25.149499325338848	28.380023480712204	23.57514550240345	22.895331691545497
22	24.162596708655215	25.394804386790316	21.865185269368457	28.577413635186012
23	24.1789214860074	31.373862442321528	20.575431547174755	23.871784524496313
24	22.894175955095786	22.659946701287396	35.73358926474937	18.712288078867456
25	19.31042984727906	25.560219166155413	32.94152262498567	22.187828361579854
26	18.663506369552508	37.22641551229463	23.540425253226672	20.56965286492619
27	21.46298898486852	33.74254429600722	26.199534045588024	18.594932673536235
28	16.962599405373595	29.240950824377176	36.950772369038205	16.84567740121102
29	17.539600827892542	25.868271085689187	35.347476979174594	21.244651107243676
30	21.389503408940968	30.502051913755096	27.711863345722186	20.39658133158175
31	33.08989029171751	26.654653428347714	19.686766528716678	20.5686897512181
32	35.28102213331613	26.739166656232932	20.876549048010254	17.103262162440686
33	30.460686179992468	27.887294507651454	20.644920201213715	21.00709911114236
34	21.14781002389485	27.224527809426764	25.563734531189958	26.063927635488426
35	22.58333100580854	24.755585818728516	28.27754818217103	24.383534993291914
36	33.90193961469673	24.58810034489102	22.305713479450525	19.204246560961728
37	23.193945096739956	32.56499332080644	25.399571799645383	18.841489782808228
38	22.81457460712184	33.587434833318724	20.32218079763151	23.275809761927924
39	23.553234665544316	31.499789559654783	23.698038811556206	21.24893696324469
40	26.412141396649712	24.209404034868566	23.66341487375024	25.71503969473148
41	17.793670224087666	23.47926753276272	25.612805174617332	33.11425706853228
42	24.277399862659983	26.67150791823935	20.66789046315175	28.38320175594891
43	23.890420774747927	26.537490645758112	23.29242347339254	26.279665106101426
44	22.055689160829395	30.553096940284064	26.268300364345915	21.122913534540626
45	19.124548901616972	36.71408717527417	19.423499396609262	24.737864526499592
46	22.229675652196526	34.003885200698456	22.67983499935953	21.086604147745494
47	23.564503095929016	25.32435261904326	26.342267497127516	24.76887678790021
48	25.874146079308563	21.086026279520638	29.72202612156999	23.31780151960081
49	21.93992289311653	23.02597806604841	30.310873842698488	24.723225198136568
50	19.247297743713514	33.88113635860191	24.362105713286827	22.50946018439775
51	19.180505808057216	34.02834828888403	22.075962704384768	24.715183198673984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	284.0
1	673.5
2	1063.0
3	2863.5
4	4664.0
5	3522.5
6	2381.0
7	2611.0
8	2841.0
9	3101.0
10	3361.0
11	3500.0
12	3639.0
13	3643.0
14	3647.0
15	3380.0
16	3113.0
17	3406.0
18	3699.0
19	3365.0
20	3031.0
21	3394.5
22	3758.0
23	3881.5
24	4005.0
25	6695.5
26	9479.0
27	9572.0
28	13185.0
29	16798.0
30	19831.0
31	22864.0
32	27326.0
33	31788.0
34	32908.5
35	34029.0
36	39357.0
37	44685.0
38	59594.0
39	74503.0
40	112489.5
41	150476.0
42	180400.0
43	210324.0
44	213363.5
45	216403.0
46	197890.5
47	179378.0
48	167908.0
49	156438.0
50	144971.0
51	133504.0
52	130152.5
53	126801.0
54	114626.0
55	102451.0
56	100813.5
57	99176.0
58	103432.5
59	107689.0
60	102414.0
61	97139.0
62	85301.0
63	73463.0
64	61275.0
65	49087.0
66	40363.0
67	31639.0
68	26696.0
69	21753.0
70	19206.5
71	16660.0
72	13826.0
73	10992.0
74	8138.0
75	4114.0
76	2944.0
77	2027.5
78	1111.0
79	811.0
80	511.0
81	328.5
82	146.0
83	116.0
84	86.0
85	54.0
86	22.0
87	14.5
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2076598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.26480829470946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77928088806722	12.02550620351415
2	8.751842330776114	2.6719039080964126
3	2.8732598410940993	1.3157928196556639
4	1.3275427830354127	0.8105874434424262
5	0.77373733839388	0.5905476070520661
6	0.4882694560103753	0.4472003785296272
7	0.3362403175954329	0.35928507923325553
8	0.25636187976410313	0.31306519589363124
9	0.21703376336887972	0.29816809121747573
>10	2.652503737666998	10.033998474197166
>50	1.44721873494716	16.185166867867334
>100	2.077403486985174	52.66216395163749
>500	0.01758174209031586	1.79805363290842
>1k	0.0017237002049329275	0.48856034675491017
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2326	0.11201012425129948	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2129	0.10252345422657637	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.051767361810037375	0.0
2	0.0	0.0	0.0	0.1786575928513848	0.0
3	0.0	0.0	0.0	0.27188699979485675	0.0
4	0.0	0.0	0.0	0.45170032909595403	0.0
5	0.0	0.0	0.0	0.7768956726337982	0.0
6	0.0	0.0	0.0	1.0962641782376752	0.0
7	0.0	0.0	0.0	1.2576820357141825	0.0
8	0.0	0.0	0.0	1.6346928967474688	0.0
9	0.0	0.0	0.0	1.7383239317383528	0.0
10	0.0	0.0	0.0	1.9342212599646151	0.0
11	0.0	0.0	0.0	2.2206994324370917	0.0
12	0.0	0.0	0.0	2.465667404090729	0.0
13	0.0	0.0	0.0	2.559522834944462	0.0
14	0.0	0.0	0.0	2.59814369463902	0.0
15	0.0	0.0	0.0	2.6632501813061555	0.0
16	0.0	0.0	0.0	2.802997980350554	0.0
17	0.0	0.0	0.0	2.9645603048832756	0.0
18	0.0	0.0	0.0	3.1560754657377115	0.0
19	0.0	0.0	0.0	3.2720825118776	0.0
20	0.0	0.0	0.0	3.3997913895708267	0.0
21	4.815568540468593E-5	0.0	0.0	3.550181595089661	0.0
22	4.815568540468593E-5	0.0	0.0	3.702112782541445	0.0
23	4.815568540468593E-5	0.0	0.0	3.8711392383118928	0.0
24	4.815568540468593E-5	0.0	0.0	3.996247708993267	0.0
25	4.815568540468593E-5	0.0	0.0	4.098385917736605	0.0
26	4.815568540468593E-5	0.0	0.0	4.196334581849737	0.0
27	9.631137080937186E-5	0.0	0.0	4.289756611534828	0.0
28	9.631137080937186E-5	0.0	0.0	4.4004183765947955	0.0
29	9.631137080937186E-5	0.0	0.0	4.51873689563411	0.0
30	9.631137080937186E-5	0.0	0.0	4.65973674249903	0.0
31	9.631137080937186E-5	0.0	0.0	4.800255032509903	0.0
32	9.631137080937186E-5	0.0	0.0	4.9233409644042805	0.0
33	9.631137080937186E-5	0.0	0.0	5.050905375041293	0.0
34	9.631137080937186E-5	0.0	0.0	5.1898345274338125	0.0
35	9.631137080937186E-5	0.0	0.0	5.357657091069143	0.0
36	9.631137080937186E-5	0.0	0.0	5.5158003619381315	0.0
37	9.631137080937186E-5	0.0	0.0	5.668453884670986	0.0
38	9.631137080937186E-5	0.0	0.0	5.806805168838649	0.0
39	9.631137080937186E-5	0.0	0.0	5.955943326536961	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	40	6.8193913E-9	45.000004	1
CCACTAG	40	6.8193913E-9	45.000004	1
CTACGAT	20	7.0346013E-4	45.000004	19
CGCTAAC	40	6.8193913E-9	45.000004	37
CGTTTAG	20	7.0346013E-4	45.000004	30
TGGTCGA	20	7.0346013E-4	45.000004	26
TACGTCT	20	7.0346013E-4	45.000004	36
TACGTCG	20	7.0346013E-4	45.000004	37
ACTATCG	25	3.891898E-5	45.0	11
CGAACTA	25	3.891898E-5	45.0	28
CGCATTG	25	3.891898E-5	45.0	39
GTTAGTA	25	3.891898E-5	45.0	22
CATTCGA	25	3.891898E-5	45.0	31
GTGTCGA	45	3.8562575E-10	45.0	14
GTACTCG	25	3.891898E-5	45.0	1
ACTCGTT	25	3.891898E-5	45.0	32
CATCGTA	35	1.2125565E-7	45.0	18
ACCGTAT	25	3.891898E-5	45.0	6
ATACGTC	35	1.2125565E-7	45.0	37
GCTCGAT	25	3.891898E-5	45.0	19
>>END_MODULE