##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545306_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1974748
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39217611563602	31.0	31.0	33.0	30.0	34.0
2	31.705919438834727	31.0	31.0	34.0	30.0	34.0
3	31.778734805656214	31.0	31.0	34.0	30.0	34.0
4	30.284517822020835	35.0	33.0	37.0	0.0	37.0
5	32.888280428692674	35.0	33.0	37.0	19.0	37.0
6	34.54714069845874	35.0	33.0	37.0	30.0	37.0
7	35.555754455758404	37.0	35.0	37.0	33.0	37.0
8	35.76080087180744	37.0	35.0	37.0	35.0	37.0
9	37.8370637671237	39.0	38.0	39.0	35.0	39.0
10	37.34127949490264	39.0	37.0	39.0	35.0	39.0
11	36.78423019038379	39.0	35.0	39.0	33.0	39.0
12	35.129792763431084	35.0	35.0	39.0	31.0	39.0
13	34.51171947002858	35.0	33.0	39.0	27.0	39.0
14	35.16047060181856	35.0	33.0	40.0	27.0	41.0
15	35.65851389645666	35.0	34.0	40.0	30.0	41.0
16	35.94861015177633	35.0	34.0	40.0	32.0	41.0
17	35.9341399510216	35.0	34.0	40.0	32.0	41.0
18	35.929075507355876	36.0	35.0	40.0	31.0	41.0
19	35.8778866974419	36.0	35.0	40.0	31.0	41.0
20	35.72243192549125	35.0	34.0	40.0	31.0	41.0
21	35.52829069835746	35.0	34.0	40.0	31.0	41.0
22	35.36576616358138	35.0	34.0	40.0	30.0	41.0
23	35.40164415915347	35.0	34.0	40.0	31.0	41.0
24	35.30364330030971	35.0	34.0	40.0	30.0	41.0
25	35.19161887997861	35.0	34.0	39.0	30.0	41.0
26	35.022342597637774	35.0	34.0	39.0	30.0	41.0
27	35.0055201980202	35.0	34.0	39.0	30.0	41.0
28	35.10692250352957	35.0	34.0	39.0	30.0	41.0
29	35.23138091543832	35.0	34.0	39.0	30.0	41.0
30	35.2314102862745	36.0	34.0	39.0	30.0	41.0
31	35.03455782712528	35.0	34.0	39.0	30.0	41.0
32	34.86394213337601	35.0	34.0	39.0	29.0	41.0
33	34.75023142193333	35.0	34.0	39.0	29.0	41.0
34	34.71104566253517	35.0	34.0	39.0	29.0	41.0
35	34.553837249107225	35.0	34.0	39.0	29.0	41.0
36	34.28810562157805	35.0	33.0	39.0	27.0	41.0
37	34.20117807436696	35.0	33.0	39.0	27.0	41.0
38	34.24991593864129	35.0	33.0	39.0	27.0	41.0
39	34.243731858444725	35.0	33.0	39.0	27.0	41.0
40	34.07366408270828	35.0	33.0	38.0	26.0	41.0
41	34.12757121414985	35.0	33.0	38.0	27.0	41.0
42	34.0847495477904	35.0	33.0	38.0	27.0	41.0
43	33.994734011630854	35.0	33.0	38.0	27.0	41.0
44	33.918954215930334	35.0	33.0	38.0	27.0	41.0
45	33.80985877691736	35.0	33.0	38.0	27.0	40.0
46	33.799911684933974	35.0	33.0	38.0	27.0	40.0
47	33.74809798516064	35.0	33.0	38.0	27.0	40.0
48	33.71660890402218	35.0	33.0	38.0	27.0	40.0
49	33.73603821854738	35.0	33.0	37.0	26.0	40.0
50	33.5443732567396	35.0	33.0	37.0	26.0	40.0
51	33.46292083850699	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	1.0
14	14.0
15	37.0
16	105.0
17	339.0
18	746.0
19	1593.0
20	2839.0
21	4641.0
22	7268.0
23	11167.0
24	15800.0
25	22284.0
26	29028.0
27	35447.0
28	42515.0
29	51904.0
30	64110.0
31	82729.0
32	108352.0
33	170570.0
34	271617.0
35	391352.0
36	121380.0
37	140717.0
38	180473.0
39	217532.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.248315228069604	25.702317460253155	30.88252273201441	13.166844579662824
2	27.832209476854768	26.13589176948147	32.61373096719176	13.418167786471995
3	27.886899999392327	25.929688243765785	31.64099925661401	14.542412500227877
4	21.759941015258654	38.57352938197684	27.028056238061772	12.638473364702737
5	23.702112877187368	32.297285527064716	29.97729330527237	14.023308290475544
6	21.70185765474886	40.91048579363037	25.731384460194413	11.656272091426349
7	90.22239799711153	4.094243923781668	4.156732909718101	1.5266251693887016
8	92.99181465179355	2.0313984366612856	3.3030543644049772	1.6737325471401923
9	90.34773044459344	3.141818601664617	4.6029670621264085	1.9074838916155377
10	70.26856084928305	14.60383805933719	7.145367408904832	7.982233682474929
11	64.48316443414552	13.848703733337114	12.386998239775405	9.28113359274196
12	54.88816800928523	20.471827291380976	14.518763913167653	10.121240786166133
13	19.217084914125753	53.58334329240997	16.16170772169411	11.037864071770171
14	10.903922930925871	52.92179052719638	24.22354649808482	11.95074004379293
15	10.277513890379938	19.65091242021767	58.0732326352527	11.998341054149694
16	11.406518705171495	13.678504801625321	55.13012293214121	19.78485356106197
17	13.914484278500344	15.643869496259777	24.409950029067	46.03169619617288
18	22.656966863620067	22.555637478807423	33.46356092017817	21.32383473739434
19	34.69708540026373	23.44385207631556	20.23817722565107	21.62088529776964
20	37.20570928543794	22.471272283855964	19.940721550294015	20.38229688041208
21	22.23301403520854	30.21509579956531	24.65109472195946	22.900795443266688
22	23.155815324284415	24.88185834344433	22.057042215006675	29.90528411726458
23	19.89013281694677	33.88579200991722	20.369383840368492	25.854691332767523
24	20.529049782554534	21.868309272879376	41.637160792161836	15.965480152404258
25	15.400749867831237	25.325408609098478	37.60727951110724	21.666562011963045
26	14.94246354471558	41.35682122478413	23.891807967396346	19.80890726310395
27	16.17375989240146	37.74106873383338	27.97430355670698	18.11086781705818
28	12.753171543913453	28.86763273085984	44.20219693854609	14.176998786680631
29	12.171071954497485	23.82324225673352	42.68934567853721	21.31634011023179
30	18.339960339243287	32.026769997994684	30.4994358773879	19.133833785374133
31	34.59190742312437	25.59045508591476	21.21571967663722	18.601917814323652
32	35.565297445547486	26.550577592685244	22.247041141451973	15.6370838203153
33	31.24272059017151	26.907686449106414	22.41617664633665	19.43341631438543
34	18.231389524131686	27.199267957227963	27.434589122257623	27.134753396382727
35	19.745658686576718	24.432585828672824	32.431429225399896	23.390326259350562
36	35.85358739444223	21.21835292401866	24.72842104410284	18.19963863743627
37	20.73586098074286	33.187323141990774	27.852515865315475	18.22430001195089
38	19.691930312120835	35.00026332473814	21.468106310273512	23.839700052867503
39	20.528188913218294	34.65748541079672	24.38265540717094	20.431670268814045
40	26.69140568821946	24.57224921863448	21.730772736571957	27.005572356574103
41	15.86384693135529	22.00523813671415	25.164426043221717	36.96648888870884
42	23.7163931802944	24.8655524654285	20.543026249425242	30.87502810485186
43	23.038433258319543	27.77225245955433	22.335862601202784	26.85345168092334
44	19.947304668747606	33.03140451338601	26.86150334118581	20.15978747668057
45	16.02781722022253	42.17915399838359	18.58824518368926	23.20478359770462
46	22.565208320251497	34.74939587228345	24.063829916526057	18.621565890938996
47	22.42529173342624	25.571060206163015	26.379213955400893	25.624434105009854
48	24.89203685736104	18.73666918513147	33.128632109008336	23.24266184849915
49	20.501046209440393	20.793893701879938	33.52280898626053	25.182251102419144
50	17.545884335621558	36.70513908610111	23.86441206675485	21.884564511522484
51	17.162088529776966	38.970542064101345	20.207287208291895	23.660082197829798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338.0
1	531.0
2	724.0
3	2337.0
4	3950.0
5	3132.0
6	2314.0
7	2743.5
8	3173.0
9	3675.5
10	4178.0
11	4414.0
12	4650.0
13	4525.5
14	4401.0
15	4320.5
16	4240.0
17	3858.5
18	3477.0
19	3309.5
20	3142.0
21	3744.0
22	4346.0
23	3620.0
24	2894.0
25	4119.5
26	6348.5
27	7352.0
28	7730.0
29	8108.0
30	11209.0
31	14310.0
32	20695.0
33	27080.0
34	30286.5
35	33493.0
36	37081.5
37	40670.0
38	61994.0
39	83318.0
40	128604.5
41	173891.0
42	219118.5
43	264346.0
44	258398.5
45	252451.0
46	223638.5
47	194826.0
48	181949.0
49	169072.0
50	156680.5
51	144289.0
52	126036.5
53	107784.0
54	96885.0
55	85986.0
56	81707.5
57	77429.0
58	68054.5
59	58680.0
60	55885.5
61	53091.0
62	49315.0
63	45539.0
64	40018.0
65	34497.0
66	27016.0
67	19535.0
68	15336.5
69	11138.0
70	9601.5
71	8065.0
72	7420.0
73	6775.0
74	4950.5
75	2319.5
76	1513.0
77	1039.0
78	565.0
79	368.5
80	172.0
81	276.0
82	380.0
83	207.5
84	35.0
85	31.5
86	28.0
87	24.5
88	21.0
89	15.0
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1974748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.779805768785728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85918775140172	11.655234780499239
2	9.592514417008296	2.83550999835319
3	3.1798505221588744	1.4099271927384012
4	1.4226465032453424	0.8410575598243342
5	0.7520960298467921	0.5557916620305231
6	0.4607759894127016	0.40861077758638786
7	0.35390419929793177	0.3661444728466747
8	0.23244490824730824	0.2748392476670748
9	0.18909669828515868	0.25153312249559906
>10	1.971078667997685	6.842370973304239
>50	0.8595527307465826	9.355612951263405
>100	2.09772685913822	62.256939134723524
>500	0.027257753776626925	2.5516762931770116
>1k	0.0018669694367552687	0.39475183349039
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0645145608452319	0.0
2	0.0	0.0	0.0	0.21815441767759736	0.0
3	0.0	0.0	0.0	0.3040387938106533	0.0
4	0.0	0.0	0.0	0.5112551069807388	0.0
5	0.0	0.0	0.0	0.802077024511482	0.0
6	0.0	0.0	0.0	1.1070779664038146	0.0
7	0.0	0.0	0.0	1.2749474869704893	0.0
8	0.0	0.0	0.0	1.7030527439450502	0.0
9	0.0	0.0	0.0	1.8222071879551214	0.0
10	0.0	0.0	0.0	2.0153963948817775	0.0
11	0.0	0.0	0.0	2.3460714987431306	0.0
12	0.0	0.0	0.0	2.6024080034515795	0.0
13	0.0	0.0	0.0	2.706725111254702	0.0
14	0.0	0.0	0.0	2.7462238219762725	0.0
15	0.0	0.0	0.0	2.8066872330039074	0.0
16	0.0	0.0	0.0	2.9500472971741205	0.0
17	0.0	0.0	0.0	3.1200943107677537	0.0
18	0.0	0.0	0.0	3.317030831275687	0.0
19	0.0	0.0	0.0	3.4364891115220777	0.0
20	0.0	0.0	0.0	3.5708353673481374	0.0
21	0.0	0.0	0.0	3.7345524593517756	0.0
22	0.0	0.0	0.0	3.90277645552749	0.0
23	0.0	0.0	0.0	4.098092516108385	0.0
24	0.0	0.0	0.0	4.2324894113071645	0.0
25	0.0	0.0	0.0	4.343440276936602	0.0
26	0.0	0.0	0.0	4.449681680903082	0.0
27	0.0	0.0	0.0	4.54680799777997	0.0
28	0.0	0.0	0.0	4.663582391272203	0.0
29	0.0	0.0	0.0	4.778381849228357	0.0
30	0.0	0.0	0.0	4.940883596286716	0.0
31	0.0	0.0	0.0	5.09614391304612	0.0
32	0.0	0.0	0.0	5.2236538535549855	0.0
33	0.0	0.0	0.0	5.349695252254971	0.0
34	0.0	0.0	0.0	5.498068614324461	0.0
35	0.0	0.0	0.0	5.707107944912464	0.0
36	0.0	0.0	0.0	5.861406113590189	0.0
37	0.0	0.0	0.0	6.003905308424163	0.0
38	0.0	0.0	0.0	6.149088390012295	0.0
39	0.0	0.0	0.0	6.3059691666987385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	25	3.8918268E-5	45.000004	28
TAATACG	25	3.8918268E-5	45.000004	41
CGTATTA	50	2.1827873E-11	45.000004	23
TCCGCGT	25	3.8918268E-5	45.000004	20
CTCCGTA	25	3.8918268E-5	45.000004	3
CACGGTA	25	3.8918268E-5	45.000004	35
CCGTGCT	25	3.8918268E-5	45.000004	11
GATTTCG	25	3.8918268E-5	45.000004	10
TTCGTAC	25	3.8918268E-5	45.000004	33
CGACATA	25	3.8918268E-5	45.000004	28
CCGTAGA	25	3.8918268E-5	45.000004	45
TATTCGT	25	3.8918268E-5	45.000004	16
ACGCTTA	25	3.8918268E-5	45.000004	36
CGTACGT	25	3.8918268E-5	45.000004	26
GTATCGC	25	3.8918268E-5	45.000004	9
ACTCGAA	25	3.8918268E-5	45.000004	45
TCGTACA	25	3.8918268E-5	45.000004	34
CTCGACT	25	3.8918268E-5	45.000004	28
GTATACT	25	3.8918268E-5	45.000004	42
CGTCGAA	25	3.8918268E-5	45.000004	31
>>END_MODULE