FastQCFastQC Report
Thu 26 May 2016
SRR1545305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545305_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2387364
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41290.1729522603172369No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39490.16541256381515346No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT35450.148490133888255No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.10316818047017547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG1250.045.0000041
GTCCGTT351.2126657E-745.00000410
CTATTCG453.8562575E-1045.00000412
TGTCGAC351.2126657E-745.00000418
TCGAACA453.8562575E-1045.0000041
ATCGATC351.2126657E-745.00000412
TATTTCG207.0348155E-445.044
ATTCGTT253.8920727E-545.014
TACGCTA253.8920727E-545.019
TACGCAT207.0348155E-445.044
CGAATCG207.0348155E-445.09
CTACCGT207.0348155E-445.031
ACTCGCT253.8920727E-545.034
CGTAACA253.8920727E-545.035
CGTAAAC207.0348155E-445.044
TGTACGA253.8920727E-545.019
CCGTTAA207.0348155E-445.030
TGTTTCG302.1664891E-644.9999961
GTCGACG302.1664891E-644.9999961
TTGCGAA302.1664891E-644.99999612