##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545305_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2387364
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31607748127223	31.0	31.0	33.0	30.0	34.0
2	31.63338309533025	31.0	31.0	34.0	30.0	34.0
3	31.73350314405344	31.0	31.0	34.0	30.0	34.0
4	30.327295711923277	35.0	33.0	37.0	0.0	37.0
5	32.90448461147944	35.0	33.0	37.0	19.0	37.0
6	34.54361128005616	35.0	33.0	37.0	30.0	37.0
7	35.519182663389415	36.0	35.0	37.0	33.0	37.0
8	35.68989437722944	37.0	35.0	37.0	35.0	37.0
9	37.70604063728866	39.0	37.0	39.0	35.0	39.0
10	37.185478628311394	39.0	37.0	39.0	33.0	39.0
11	36.75894208005147	39.0	35.0	39.0	32.0	39.0
12	35.63534551078093	37.0	35.0	39.0	31.0	39.0
13	35.187219879331344	37.0	35.0	39.0	30.0	39.0
14	36.02701347595088	38.0	35.0	40.0	30.0	41.0
15	36.34567749199535	38.0	35.0	40.0	31.0	41.0
16	36.52827428075484	38.0	35.0	40.0	32.0	41.0
17	36.45713808200174	37.0	35.0	40.0	32.0	41.0
18	36.446403229670885	37.0	35.0	40.0	31.0	41.0
19	36.423622874433896	37.0	35.0	40.0	31.0	41.0
20	36.31637320492393	37.0	35.0	40.0	31.0	41.0
21	36.1508454513011	37.0	34.0	40.0	31.0	41.0
22	36.03846376170538	37.0	34.0	40.0	30.0	41.0
23	35.974837938412406	36.0	34.0	40.0	30.0	41.0
24	35.906058313688234	36.0	34.0	40.0	30.0	41.0
25	35.77762670459972	36.0	34.0	40.0	30.0	41.0
26	35.63644463098212	36.0	34.0	40.0	30.0	41.0
27	35.636620138361806	36.0	34.0	40.0	30.0	41.0
28	35.674046772926125	36.0	34.0	40.0	30.0	41.0
29	35.73087388433435	36.0	34.0	40.0	30.0	41.0
30	35.68440254607174	36.0	34.0	40.0	30.0	41.0
31	35.582651409671925	36.0	34.0	40.0	30.0	41.0
32	35.46882712481214	36.0	34.0	40.0	30.0	41.0
33	35.326937576339425	36.0	34.0	40.0	29.0	41.0
34	35.24868474183241	36.0	34.0	40.0	29.0	41.0
35	35.13340613329178	36.0	34.0	40.0	29.0	41.0
36	34.95551243966148	35.0	34.0	40.0	27.0	41.0
37	34.91378817809098	35.0	33.0	40.0	27.0	41.0
38	34.90727220482507	35.0	33.0	40.0	28.0	41.0
39	34.849945379087565	35.0	34.0	40.0	27.0	41.0
40	34.72686821113161	35.0	33.0	40.0	27.0	41.0
41	34.704478663496644	35.0	34.0	40.0	27.0	41.0
42	34.663738751191694	35.0	33.0	40.0	27.0	41.0
43	34.595803153603725	35.0	33.0	40.0	27.0	41.0
44	34.53405555248383	35.0	33.0	40.0	27.0	41.0
45	34.415334234745934	35.0	33.0	39.0	27.0	41.0
46	34.35317613903871	35.0	33.0	39.0	27.0	41.0
47	34.28823673306626	35.0	33.0	39.0	27.0	41.0
48	34.21530859977783	35.0	33.0	39.0	26.0	41.0
49	34.19300324542047	35.0	33.0	39.0	26.0	41.0
50	34.03154190144444	35.0	33.0	39.0	26.0	40.0
51	33.95661700519904	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	6.0
13	4.0
14	15.0
15	45.0
16	130.0
17	393.0
18	878.0
19	1835.0
20	3114.0
21	5359.0
22	8408.0
23	12370.0
24	17744.0
25	24360.0
26	31896.0
27	39515.0
28	48109.0
29	59003.0
30	73755.0
31	93126.0
32	120262.0
33	176589.0
34	269241.0
35	367916.0
36	177181.0
37	219367.0
38	284977.0
39	351520.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.757955636425784	26.20945109333977	30.33844022109741	11.694153049137041
2	27.889002263584437	27.299314222715932	31.511114350388127	13.3005691633115
3	29.647971570317722	25.835147049214115	30.92205461756146	13.594826762906703
4	23.527622934751466	38.33060228771147	25.55638771465097	12.585387062886094
5	23.841022986021404	33.58444711405551	28.01952278747606	14.555007112447033
6	22.17965086178731	42.03481329198229	24.875385571701674	10.910150274528727
7	89.48505548378881	4.6338974701805	4.217036028020863	1.6640110180098218
8	91.1578628143844	3.305528608121761	3.6655491160962463	1.8710594613975915
9	87.24928414770433	5.072665919398968	4.863858213494046	2.814191719402655
10	59.294351426929445	21.577061562459683	9.284172836651637	9.84441417395923
11	52.33043641438842	19.694022361064338	15.208405588758145	12.767135635789096
12	46.94424478211115	20.97618963844642	18.304079310905248	13.775486268537183
13	22.669689247219946	41.68631176477488	18.960661214628352	16.68333777337683
14	15.006383609705098	43.164301715197176	24.54263363274306	17.286681042354665
15	15.512967440239528	22.887209491305054	46.8897495312822	14.71007353717322
16	17.799045306874024	17.70572061905935	44.77867639790162	19.71655767616501
17	17.552581005661473	19.682545267500053	25.801092753346367	36.963780973492106
18	23.545885755167625	24.7537451348014	29.960617651937447	21.73975145809353
19	33.17277968504174	25.30581846756506	20.400156825687244	21.12124502170595
20	36.87439368273962	22.957747540802323	21.35870357431879	18.809155202139262
21	25.32077219896086	29.32116761415519	23.21489307872616	22.143167108157787
22	25.182586316958787	25.608495394920926	21.80576568968955	27.403152598430736
23	23.667568079270694	30.341079114873143	22.409192733072963	23.582160072783203
24	23.792768928408066	22.70822547378615	34.88944291695778	18.609562680848
25	19.895164708858808	25.004481930698464	32.428234655461004	22.672118704981727
26	18.979091583855666	36.95100537664135	23.005457064779396	21.064445974723586
27	20.648464163822524	33.556508349795	26.719260238488978	19.075767247893495
28	17.15502956398773	29.493240243213855	36.20151765713147	17.150212535666952
29	18.133556508349795	25.71706702455093	34.33213368384544	21.817242783253832
30	20.858319049797185	30.976675529998776	27.609656508182244	20.555348912021795
31	31.638116349245443	26.82016651000853	20.38415591422171	21.15756122652432
32	34.09932461074223	26.831601716370024	21.33600071040696	17.733072962480794
33	30.04874832660625	28.28961147106181	21.99346224538864	19.668177956943307
34	20.38172645645993	28.060488471804046	26.462617346998613	25.09516772473741
35	23.17941461796358	24.3032901560047	28.40463372992137	24.112661496110356
36	31.365053674261656	25.71099338014647	22.936301292974175	19.9876516526177
37	22.508632952494885	32.288415172550145	26.12035701300681	19.082594861948156
38	22.22049088450693	32.892051651947504	21.11140152905045	23.776055934495115
39	23.88395736888049	29.860632898879263	24.83626292429642	21.41914680794382
40	26.428186066305763	24.586824631685825	23.668866582557165	25.316122719451243
41	18.187255902325745	24.872202144289684	25.887296616686857	31.053245336697717
42	23.140124421747167	26.83013566427239	21.874544476669666	28.155195437310777
43	23.676992699898296	26.532233878034518	23.127265050490834	26.66350837157635
44	21.52729956554593	30.207542712380686	25.815501951105908	22.449655770967478
45	19.467663917190674	35.650826602059844	20.35651036038074	24.52499912036874
46	22.09885044760665	33.035138336675935	23.158429129366112	21.70758208635131
47	22.78910966237239	26.27190491269869	26.283005021437873	24.655980403491046
48	24.56462441420747	21.438414921226926	30.118071647222628	23.878889017342978
49	21.40779537598791	23.684993155631066	30.529362091411283	24.377849376969746
50	19.117696337885633	32.982611784378086	24.68953205292532	23.210159824810965
51	18.23391824623308	33.63039737551542	22.695617425746555	25.440066952504935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	482.0
1	883.0
2	1284.0
3	3442.0
4	5600.0
5	4213.5
6	2827.0
7	3036.5
8	3246.0
9	3541.5
10	3837.0
11	3937.5
12	4038.0
13	4117.5
14	4197.0
15	4041.5
16	3886.0
17	3579.5
18	3273.0
19	3416.0
20	3559.0
21	3933.0
22	4307.0
23	4894.0
24	5481.0
25	7236.0
26	11760.0
27	14529.0
28	19906.5
29	25284.0
30	28129.5
31	30975.0
32	36473.5
33	41972.0
34	45815.0
35	49658.0
36	52074.0
37	54490.0
38	74114.0
39	93738.0
40	130245.5
41	166753.0
42	202191.5
43	237630.0
44	236377.0
45	235124.0
46	218516.0
47	201908.0
48	194462.0
49	187016.0
50	173163.0
51	159310.0
52	149297.0
53	139284.0
54	125094.0
55	110904.0
56	111738.5
57	112573.0
58	118469.0
59	124365.0
60	117701.5
61	111038.0
62	96879.0
63	82720.0
64	67117.0
65	51514.0
66	43240.0
67	34966.0
68	30733.0
69	26500.0
70	21940.0
71	17380.0
72	13711.5
73	10043.0
74	8163.0
75	4852.5
76	3422.0
77	2657.5
78	1893.0
79	1245.0
80	597.0
81	474.0
82	351.0
83	212.0
84	73.0
85	54.0
86	35.0
87	28.0
88	21.0
89	13.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2387364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.457232488572464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48922429526881	12.132261877796802
2	9.044982707103696	2.796208011176388
3	3.0836017881361797	1.429918492241961
4	1.4582199330822647	0.9016017810049259
5	0.8041900459543101	0.6215276252655773
6	0.49884586682679055	0.46264659237030953
7	0.3434484298278235	0.3716133559378683
8	0.2728344379113636	0.33738122701479484
9	0.18746890012100298	0.2607975336192583
>10	2.4459366288669226	9.355596764563865
>50	1.341287539004641	15.234524105823969
>100	2.012816497375228	53.6989753216039
>500	0.014187252844897967	1.3306221376113414
>1k	0.00295567767602041	1.0663251739690545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4129	0.1729522603172369	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3949	0.16541256381515346	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3545	0.148490133888255	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2463	0.10316818047017547	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05922850474414459	0.0
2	0.0	0.0	0.0	0.20386501597577913	0.0
3	0.0	0.0	0.0	0.29442514840635947	0.0
4	0.0	0.0	0.0	0.47399558676431414	0.0
5	0.0	0.0	0.0	0.8205703026434176	0.0
6	0.0	0.0	0.0	1.1399183367094419	0.0
7	0.0	0.0	0.0	1.3037810740213893	0.0
8	0.0	0.0	0.0	1.685541040243549	0.0
9	0.0	0.0	0.0	1.7972960972855416	0.0
10	0.0	0.0	0.0	2.00380000703705	0.0
11	0.0	0.0	0.0	2.2980994938350414	0.0
12	0.0	0.0	0.0	2.539788653929606	0.0
13	0.0	0.0	0.0	2.6435432552388325	0.0
14	0.0	0.0	0.0	2.6851372476086595	0.0
15	4.1887202789352605E-5	0.0	0.0	2.756596815567295	0.0
16	4.1887202789352605E-5	0.0	0.0	2.9150979909222055	0.0
17	4.1887202789352605E-5	0.0	0.0	3.0942495572522666	0.0
18	8.377440557870521E-5	0.0	0.0	3.3094660051839604	0.0
19	8.377440557870521E-5	0.0	0.0	3.4313996525037656	0.0
20	8.377440557870521E-5	0.0	0.0	3.5630092436679117	0.0
21	8.377440557870521E-5	0.0	0.0	3.726494996154755	0.0
22	8.377440557870521E-5	0.0	0.0	3.9049763672401863	0.0
23	8.377440557870521E-5	0.0	0.0	4.093133682169958	0.0
24	8.377440557870521E-5	0.0	0.0	4.233874683542183	0.0
25	8.377440557870521E-5	0.0	0.0	4.354216617155993	0.0
26	8.377440557870521E-5	0.0	0.0	4.472296641819178	0.0
27	1.2566160836805784E-4	0.0	0.0	4.5780199416595035	0.0
28	1.2566160836805784E-4	0.0	0.0	4.698445649678892	0.0
29	1.2566160836805784E-4	0.0	0.0	4.8215102514740105	0.0
30	1.6754881115741042E-4	0.0	0.0	4.9790899083675555	0.0
31	1.6754881115741042E-4	0.0	0.0	5.128752883933912	0.0
32	2.0943601394676303E-4	0.0	0.0	5.269703321320083	0.0
33	2.0943601394676303E-4	0.0	0.0	5.41036054828673	0.0
34	2.0943601394676303E-4	0.0	0.0	5.556379337210413	0.0
35	2.0943601394676303E-4	0.0	0.0	5.74742686913265	0.0
36	2.0943601394676303E-4	0.0	0.0	5.917824010079737	0.0
37	2.0943601394676303E-4	0.0	0.0	6.086964534943142	0.0
38	2.0943601394676303E-4	0.0	0.0	6.241653974844222	0.0
39	2.0943601394676303E-4	0.0	0.0	6.397306820409455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	125	0.0	45.000004	1
GTCCGTT	35	1.2126657E-7	45.000004	10
CTATTCG	45	3.8562575E-10	45.000004	12
TGTCGAC	35	1.2126657E-7	45.000004	18
TCGAACA	45	3.8562575E-10	45.000004	1
ATCGATC	35	1.2126657E-7	45.000004	12
TATTTCG	20	7.0348155E-4	45.0	44
ATTCGTT	25	3.8920727E-5	45.0	14
TACGCTA	25	3.8920727E-5	45.0	19
TACGCAT	20	7.0348155E-4	45.0	44
CGAATCG	20	7.0348155E-4	45.0	9
CTACCGT	20	7.0348155E-4	45.0	31
ACTCGCT	25	3.8920727E-5	45.0	34
CGTAACA	25	3.8920727E-5	45.0	35
CGTAAAC	20	7.0348155E-4	45.0	44
TGTACGA	25	3.8920727E-5	45.0	19
CCGTTAA	20	7.0348155E-4	45.0	30
TGTTTCG	30	2.1664891E-6	44.999996	1
GTCGACG	30	2.1664891E-6	44.999996	1
TTGCGAA	30	2.1664891E-6	44.999996	12
>>END_MODULE