FastQCFastQC Report
Thu 26 May 2016
SRR1545304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545304_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2811365
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT42270.15035400952917888No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32710.1163491755784112No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC351.2127748E-745.018
TACGCTA207.03503E-445.029
TATCTCG351.2127748E-745.014
GTCGTGA253.8922506E-545.030
ATCGATT253.8922506E-545.035
CCCGATA253.8922506E-545.040
CGTAAAT650.041.5384616
CGCGAAA603.6379788E-1241.24999612
GCGATAT2850.041.052639
CGGTCTA1550.040.6451631
GTCGATA501.0822987E-940.543
ACTAGTC501.0822987E-940.515
ATTGGGA18500.040.3783764
TCGCGAA451.9299478E-840.016
AACGGGA11300.039.823014
TATAGCG1650.039.5454561
ACGGGTA1600.039.3755
CGGGATA3550.039.2957766
ATAGGGC14550.039.123714
AATGGGC8800.039.119324
Produced by FastQC (version 0.11.5)