##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545304_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2811365 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.350545375644927 31.0 31.0 33.0 30.0 34.0 2 31.677761870123586 31.0 31.0 34.0 30.0 34.0 3 31.774230311610197 31.0 31.0 34.0 30.0 34.0 4 30.316473670263377 35.0 33.0 37.0 0.0 37.0 5 32.92841235485253 35.0 33.0 37.0 19.0 37.0 6 34.58327751821624 36.0 33.0 37.0 31.0 37.0 7 35.53887061978789 36.0 35.0 37.0 33.0 37.0 8 35.70959978515774 37.0 35.0 37.0 35.0 37.0 9 37.749733314599844 39.0 37.0 39.0 35.0 39.0 10 37.22243251943451 39.0 37.0 39.0 34.0 39.0 11 36.83192257141993 39.0 35.0 39.0 33.0 39.0 12 35.83433029862718 37.0 35.0 39.0 32.0 39.0 13 35.46957972372851 37.0 35.0 39.0 31.0 39.0 14 36.32515806378752 38.0 35.0 40.0 31.0 41.0 15 36.549570048712994 38.0 35.0 40.0 31.0 41.0 16 36.70675027966842 38.0 35.0 40.0 32.0 41.0 17 36.64130449087899 38.0 35.0 40.0 32.0 41.0 18 36.61490557078145 38.0 35.0 40.0 31.0 41.0 19 36.63656729026647 38.0 35.0 40.0 31.0 41.0 20 36.537781469143994 38.0 35.0 40.0 31.0 41.0 21 36.37122892260521 38.0 35.0 40.0 31.0 41.0 22 36.23396250575788 37.0 35.0 40.0 31.0 41.0 23 36.19431806257814 37.0 35.0 40.0 31.0 41.0 24 36.14049474187806 37.0 35.0 40.0 31.0 41.0 25 36.018806167110995 37.0 34.0 40.0 30.0 41.0 26 35.87973315453525 37.0 34.0 40.0 30.0 41.0 27 35.877654093296314 37.0 34.0 40.0 30.0 41.0 28 35.91330261278774 37.0 34.0 40.0 30.0 41.0 29 35.96105912963987 37.0 35.0 40.0 30.0 41.0 30 35.89984687153749 36.0 35.0 40.0 30.0 41.0 31 35.792972452883205 36.0 34.0 40.0 30.0 41.0 32 35.68541758185081 36.0 34.0 40.0 30.0 41.0 33 35.55473053125439 36.0 34.0 40.0 30.0 41.0 34 35.46450994445759 36.0 34.0 40.0 29.0 41.0 35 35.362423235688 36.0 34.0 40.0 29.0 41.0 36 35.20113574722599 36.0 34.0 40.0 29.0 41.0 37 35.157572567062616 36.0 34.0 40.0 29.0 41.0 38 35.141600254680554 36.0 34.0 40.0 29.0 41.0 39 35.089655736626156 36.0 34.0 40.0 28.0 41.0 40 34.98828469444558 36.0 34.0 40.0 28.0 41.0 41 34.97592308362664 36.0 34.0 40.0 28.0 41.0 42 34.90252670855617 35.0 34.0 40.0 28.0 41.0 43 34.83853288349254 35.0 33.0 40.0 28.0 41.0 44 34.754645163470414 35.0 34.0 40.0 27.0 41.0 45 34.634057121718456 35.0 33.0 40.0 27.0 41.0 46 34.5712691166035 35.0 33.0 39.0 27.0 41.0 47 34.533586709658834 35.0 33.0 39.0 27.0 41.0 48 34.43336955535834 35.0 33.0 39.0 27.0 41.0 49 34.38309255468429 35.0 33.0 39.0 27.0 41.0 50 34.2364605805365 35.0 33.0 39.0 27.0 41.0 51 34.13251747816452 35.0 33.0 39.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 3.0 12 7.0 13 7.0 14 12.0 15 44.0 16 151.0 17 372.0 18 884.0 19 1724.0 20 3241.0 21 5303.0 22 8519.0 23 12822.0 24 18479.0 25 26200.0 26 34759.0 27 43243.0 28 52877.0 29 65517.0 30 82054.0 31 104739.0 32 136828.0 33 200855.0 34 305397.0 35 418399.0 36 220553.0 37 272733.0 38 355755.0 39 439608.0 40 277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.832010429097608 24.898865853419956 31.27647957486844 11.992644142613997 2 28.324426035039917 26.75010181886735 31.54848267656459 13.376989469528148 3 29.320810353689403 26.083486135738333 30.59158095800439 14.00412255256788 4 22.312684407752105 39.098196072014844 26.06417167461358 12.524947845619478 5 24.39697442345622 32.57364305239626 28.143979881658908 14.885402642488613 6 21.91252292036075 41.00449425812728 25.531690122058148 11.551292699453825 7 89.45238345074368 4.337323684402417 4.227341522712277 1.9829513421416287 8 91.50113912636743 3.026821490628218 3.5363604512398785 1.9356789317644632 9 87.819546732637 4.6606185963046425 4.9196742507643085 2.6001604202940563 10 56.64362329331125 23.11396777010456 9.345282451762756 10.897126484821431 11 49.26578370293434 19.66795489024015 16.994129186356094 14.072132220469417 12 43.62741942081516 22.551678632977218 19.50490242284442 14.315999523363207 13 23.209010569598753 39.83235901421551 20.74209503212852 16.21653538405721 14 17.30515247931165 40.515443565670054 24.976479397018885 17.202924557999406 15 16.18644324020538 23.643070181210906 43.96881941690246 16.201667161681247 16 19.347612280867125 18.621986117064132 41.22264451609805 20.807757085970692 17 19.60602767694696 20.36224396334165 25.198008796438742 34.833719563272645 18 23.298646742774416 24.664922555413472 30.02004364427956 22.01638705753255 19 31.658784967444642 25.08998297979807 21.328962977059188 21.922269075698104 20 34.237994710754386 24.5720139505187 22.064655425389446 19.125335913337473 21 26.648727575394872 28.410540787126536 24.331774778443922 20.60895685903467 22 26.33574793738984 24.68864768537703 22.64305773174241 26.332546645490712 23 23.73704588340539 28.94906922438033 22.698724640877295 24.615160251336984 24 23.598323234443054 22.400506515518263 34.43170844056179 19.56946180947689 25 21.01904235131333 25.399974745363906 30.838080434237465 22.7429024690853 26 19.95539533287211 33.67093920568834 24.075742566333435 22.297922895106115 27 19.652944388224224 32.59783059118969 27.18199166597009 20.567233354615997 28 17.341860626421685 28.431562603930832 34.91392971030087 19.312647059346617 29 18.250138278025087 25.06917458245372 34.16913136501309 22.511555774508114 30 22.15546540559479 29.639303327742926 27.678725458985227 20.526505807677054 31 31.309524021249462 26.694683899102394 21.25170513256016 20.74408694708798 32 32.52711761012888 26.238001824736383 22.63999871948324 18.59488184565149 33 29.403332544866995 27.06318816660234 22.4302073903602 21.103271898170462 34 20.92364385271923 26.811246494140743 27.282796790882717 24.982312862257302 35 22.857366439434227 24.16011439283053 28.983038488421105 23.999480679314143 36 31.023435235197137 25.91936657104289 23.063636347468222 19.993561846291747 37 23.187170644864683 31.640430893889622 25.199111463648443 19.973286997597253 38 22.562776444894205 32.397358578484116 21.699494729428586 23.34037024719309 39 22.537628518531033 30.693133051026816 24.792974231378707 21.976264199063444 40 25.50291406487596 25.27576461967763 23.676470326691838 25.544850988754575 41 18.589332939692994 23.89938695260132 25.12594415879831 32.38533594890738 42 22.186019958276496 25.510952864533778 23.32379466913759 28.97923250805214 43 22.507820933959128 25.668883264890894 24.565362377350507 27.257933423799468 44 20.91215477179235 28.5904178219477 27.113128320228785 23.384299086031163 45 19.307418282578038 33.90726568766418 21.972387078874497 24.81292895088329 46 21.752031486484324 31.62776800593306 24.474730246695113 22.145470260887503 47 22.80155725065938 25.270037864169186 26.305122244888157 25.62328264028328 48 23.381560202961907 21.712726735944994 30.884677016324808 24.021036044768287 49 21.891216544276535 24.016660945839476 30.510517133136396 23.581605376747593 50 20.7418104728486 30.846901771915064 25.71796974067757 22.693318014558763 51 19.080197697559726 32.26091240376116 24.119244566251623 24.539645332427487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 564.0 1 1102.0 2 1640.0 3 4539.5 4 7439.0 5 5504.0 6 3569.0 7 3795.5 8 4022.0 9 4322.0 10 4622.0 11 4669.5 12 4717.0 13 4707.0 14 4697.0 15 4360.5 16 4024.0 17 4212.5 18 4401.0 19 4441.0 20 4481.0 21 4433.5 22 4386.0 23 5168.0 24 5950.0 25 8558.5 26 14552.0 27 17937.0 28 22135.0 29 26333.0 30 30484.0 31 34635.0 32 42010.5 33 49386.0 34 51587.5 35 53789.0 36 60388.0 37 66987.0 38 86469.5 39 105952.0 40 149706.5 41 193461.0 42 224492.0 43 255523.0 44 257829.0 45 260135.0 46 247414.5 47 234694.0 48 226615.5 49 218537.0 50 210716.0 51 202895.0 52 191172.5 53 179450.0 54 168508.5 55 157567.0 56 152656.5 57 147746.0 58 142675.5 59 137605.0 60 127333.0 61 117061.0 62 104784.0 63 92507.0 64 80695.5 65 68884.0 66 58813.5 67 48743.0 68 38976.5 69 29210.0 70 23805.0 71 18400.0 72 15537.0 73 12674.0 74 10177.5 75 6222.0 76 4763.0 77 3384.0 78 2005.0 79 1344.5 80 684.0 81 497.0 82 310.0 83 194.0 84 78.0 85 52.0 86 26.0 87 20.0 88 14.0 89 11.0 90 8.0 91 6.0 92 4.0 93 2.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2811365.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.376823160686454 #Duplication Level Percentage of deduplicated Percentage of total 1 78.279245521522 12.03686115536401 2 9.025834043739067 2.7757730793655835 3 3.0333574518180075 1.3993020335926796 4 1.4313550905600607 0.880387764307616 5 0.7912233075123263 0.6083250440115241 6 0.5061968446124084 0.4670219618461491 7 0.3643998304208987 0.39223182265164036 8 0.2663106096266345 0.3276008920034689 9 0.20433037574024096 0.2827756848702873 >10 2.6306449843573603 10.145233320745778 >50 1.4310030398221958 16.05899187766948 >100 2.020421039893024 52.5098617983401 >500 0.012643435786915657 1.2101221800613884 >1k 0.003034424588859758 0.9055113851702851 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 4227 0.15035400952917888 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3271 0.1163491755784112 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.07665315602918867 0.0 2 0.0 0.0 0.0 0.2556409431005935 0.0 3 0.0 0.0 0.0 0.3672948905602794 0.0 4 0.0 0.0 0.0 0.5467450864615587 0.0 5 0.0 0.0 0.0 0.8907416859781636 0.0 6 0.0 0.0 0.0 1.2072071751622433 0.0 7 0.0 0.0 0.0 1.3800057978953284 0.0 8 0.0 0.0 0.0 1.788810773414338 0.0 9 0.0 0.0 0.0 1.9108867045011944 0.0 10 0.0 0.0 0.0 2.1218162707439268 0.0 11 3.556990999034277E-5 0.0 0.0 2.4305986593700926 0.0 12 3.556990999034277E-5 0.0 0.0 2.66902376603536 0.0 13 3.556990999034277E-5 0.0 0.0 2.7764093242962047 0.0 14 3.556990999034277E-5 0.0 0.0 2.8205515825942205 0.0 15 7.113981998068554E-5 0.0 0.0 2.900014761512646 0.0 16 7.113981998068554E-5 0.0 0.0 3.0602927759291303 0.0 17 7.113981998068554E-5 0.0 0.0 3.2386758745306996 0.0 18 7.113981998068554E-5 0.0 0.0 3.4471511169840983 0.0 19 7.113981998068554E-5 0.0 0.0 3.5749538035794 0.0 20 7.113981998068554E-5 0.0 0.0 3.699341778815629 0.0 21 1.0670972997102831E-4 0.0 0.0 3.8765510703875163 0.0 22 1.0670972997102831E-4 0.0 0.0 4.0564281052086795 0.0 23 1.0670972997102831E-4 0.0 0.0 4.251849190695623 0.0 24 1.0670972997102831E-4 0.0 0.0 4.406613869063604 0.0 25 1.0670972997102831E-4 0.0 0.0 4.5379379767479495 0.0 26 1.0670972997102831E-4 0.0 0.0 4.663677608563812 0.0 27 1.0670972997102831E-4 0.0 0.0 4.776042954223304 0.0 28 1.0670972997102831E-4 0.0 0.0 4.906548953977872 0.0 29 1.0670972997102831E-4 0.0 0.0 5.0454494524901605 0.0 30 1.0670972997102831E-4 0.0 0.0 5.213303857734588 0.0 31 1.0670972997102831E-4 0.0 0.0 5.370167160792 0.0 32 1.0670972997102831E-4 0.0 0.0 5.516893039502164 0.0 33 1.4227963996137107E-4 0.0 0.0 5.667745027771208 0.0 34 2.1341945994205662E-4 0.0 0.0 5.826244546688175 0.0 35 2.1341945994205662E-4 0.0 0.0 6.022732729474828 0.0 36 2.1341945994205662E-4 0.0 0.0 6.1933971576084925 0.0 37 2.1341945994205662E-4 0.0 0.0 6.363705886642254 0.0 38 2.8455927992274214E-4 0.0 0.0 6.530528764496961 0.0 39 3.2012918991308493E-4 0.0 0.0 6.711472896617835 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGAAC 35 1.2127748E-7 45.0 18 TACGCTA 20 7.03503E-4 45.0 29 TATCTCG 35 1.2127748E-7 45.0 14 GTCGTGA 25 3.8922506E-5 45.0 30 ATCGATT 25 3.8922506E-5 45.0 35 CCCGATA 25 3.8922506E-5 45.0 40 CGTAAAT 65 0.0 41.53846 16 CGCGAAA 60 3.6379788E-12 41.249996 12 GCGATAT 285 0.0 41.05263 9 CGGTCTA 155 0.0 40.64516 31 GTCGATA 50 1.0822987E-9 40.5 43 ACTAGTC 50 1.0822987E-9 40.5 15 ATTGGGA 1850 0.0 40.378376 4 TCGCGAA 45 1.9299478E-8 40.0 16 AACGGGA 1130 0.0 39.82301 4 TATAGCG 165 0.0 39.545456 1 ACGGGTA 160 0.0 39.375 5 CGGGATA 355 0.0 39.295776 6 ATAGGGC 1455 0.0 39.12371 4 AATGGGC 880 0.0 39.11932 4 >>END_MODULE